The brown alga Spatoglossum schroederi contains three fractions of sulfated polysaccharides. One them was purified by acetone fractionation, ion exchange, and molecular sieving chromatography. It has a size 21.5 kDa fucose, xylose, galactose, sulfate in molar ratio 1.0:0.5:2.0:2.0 trace amounts glucuronic acid. Chemical analyses, methylation studies, NMR spectroscopy showed that the polysaccharide unique structure, composed central core formed mainly 4-linked beta-galactose units, partially...

Baccharis dracunculifolia DC (Asteraceae) is the main botanical source used by honeybees to produce Brazilian green propolis whose hepatoprotective properties have been already described. In this work we investigated protective effects of glycolic extract B. (GEBd) against oxidative stress in isolated rat liver mitochondria (RLM). The GEBd was prepared fractionated percolation using propylene glycol as solvent. total phenols and flavonoids, which are substances with recognized antioxidant...

Sepsis is a dysregulated host response to infection and major cause of death worldwide. Respiratory tract infections account for most sepsis cases depending on the place acquisition, i.e., community or hospital acquired infection, differ in etiology, antimicrobial resistance outcomes. Accordingly, may be different septic patients secondary community-acquired pneumonia (HAP). Proteomic analysis useful approach evaluate broad alterations biological pathways that take during sepsis. Here we...

Sepsis is a life-threatening disorder characterized by organ dysfunction and major cause of mortality worldwide. The challenge in studying sepsis its diversity such factors as age, source infection etiology. Recently, genomic proteomic approaches have improved our understanding complex pathogenesis. In the present study, we use quantitative proteomics to evaluate host proteome response septic patients secondary community-acquired pneumonia (CAP). Samples obtained at admission after 7 days...

Antivenoms are the only effective treatment for snakebite envenomation and have saved countless lives over more than a century. Despite their value, antivenoms present risks of adverse reactions. Current formulations contain fraction nonspecific antibodies serum proteins. While new promising candidates emerge as next generation antivenoms, it remains clear that animal-derived will still play critical role years to come. In this study, we improved bothropic antivenom (BAv), by capturing...

The kinetics of nanoparticle–protein interactions is modulated by nanoparticle surface chemistry.

Kazal-type inhibitors play several important roles in invertebrates, such as anticoagulant, vasodilator and antimicrobial activities. Putative were described insect transcriptomes. In this paper we characterized for the first time a Kazal unique domain trypsin inhibitor from Aedes aegypti mosquito. Previously, analyses of sialotranscriptome A. showed potential presence serine protease inhibitor, female salivary glands, carcass also whole male, which named AaTI (A. inhibitor). sequence amino...

Rhipicephalus (Boophilus) microplus is an ectoparasite responsible for important decrease in meat, milk and leather production, caused both by cattle blood loss the transmission of anaplasmosis babesiosis. R. a rich source serine protease inhibitors, including trypsin inhibitors BmTI-A BmTI-6, subtilisin inhibitor BmSI, recently described thrombin inhibitor, boophilin. Boophilin double Kunitz-type with unusual ability to form ternary complex second (non-thrombin) proteinase molecule. The...

The catalytic activity of enzymes can be regulated by interactions with synthetic nanoparticles (NPs) in a number ways. To date, however, the potential use NPs as allosteric effectors has not been investigated detail. Importantly, targeting (distal) sites on enzyme surface could afford unique ways to modulate activity, allowing for either activation, partial or full inhibition. Using p-mercaptobenzoic acid-coated ultrasmall gold (AuMBA) and human α-thrombin model system, here we...

The major goal in restorative dentistry is to develop a true regenerative approach that fully recovers hydroxyapatite crystals within the caries lesion. Recently, rationally designed self-assembling peptide P 11 -4 (Ace-QQRFEWEFEQQ-NH 2 ) has been developed enhance remineralization on initial lesions, yet its applicability dentin tissues remains unclear. Thus, present study investigated interaction of with organic components as well effect proteolytic activity, mechanical properties bonding...

Ultrasmall gold nanoparticles (usNPs) and nanoclusters are an emerging class of nanomaterials exhibiting distinctive physicochemical properties in vivo behaviors. Although understanding the interactions usNPs with blood components is fundamental importance to advance their clinical translation, currently, little known about way that interact hemostatic system. This study describes effects a model anionic p-mercaptobenzoic acid-coated usNP on coagulation cascade, particular emphasis contact...

To date, much effort has been devoted toward the study of protein corona formation onto large gold nanoparticles (GNPs). However, concept breaks down for GNPs in ultrasmall size regime (<3 nm), and, as a result, our understanding GNP (usGNP)-protein interactions remains incomplete. Herein, we used anionic usGNPs and six different proteins model systems to systematically investigate usGNP-protein interactions, with particular focus on time evolution long-term behavior complex formation. The...

The recombinant phage antibody system pCANTAB 5E has been used to display functionally active leech‐derived tryptase inhibitor (LDTI) on the tip of filamentous M13 phage. A limited combinatorial library 5.2×10 4 mutants was created with a synthetic LDTI gene, using degenerated oligonucleotide and phagemid. mutations were restricted P1–P4′ positions reactive site. Fusion phages appropriate host strains containing phagemids selected after binding thrombin DNA sequencing. variants LDTI‐2T (K8R,...

The tick Rhipicephalus (Boophilus) microplus is one of the most important bovine ectoparasites, a disease vector responsible for losses in meat and milk productions. A cysteine protease similar to cathepsin L, named BmCL1, was previously identified R. gut, suggesting role enzyme meal digestion. In this work, BmCL1 successfully expressed Pichia pastoris system, yielding 54.8 mg/L culture its activity analyzed by synthetic substrates against inhibitor, Bmcystatin. After rBmCl1 biochemical...

Blood coagulation is an important process in haemostasis, and disorders of blood can lead to increased risk haemorrhage thrombosis. Coagulation highly conserved mammals has been comprehensively studied humans the investigation bleeding or thrombotic diseases. Some substances act as inhibitors may affect one multiple enzymes throughout process. A specific thrombin inhibitor called infestin isolated from midgut haematophagous insect Triatoma infestans. Infestin a member nonclassical Kazal-type...

Gold nanoparticles (NPs) in the ultrasmall size regime provide a new paradigm way that nanomaterials can be used to regulate protein structure and function. However, rational design of NPs as viable synthetic effectors function requires detailed quantitative understanding their biomolecular interactions. Herein, we focused on kinetics NP–protein complexation—an often neglected factor studies at bio-nanointerface—to gain molecular-level insights into mechanisms The α-thrombin gold coated with...