A5002864713- Insect Resistance and Genetics
- Glycosylation and Glycoproteins Research
- Blood Coagulation and Thrombosis Mechanisms
- Protease and Inhibitor Mechanisms
- Protein Interaction Studies and Fluorescence Analysis
- Biochemical and Structural Characterization
- Moringa oleifera research and applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Monoclonal and Polyclonal Antibodies Research
- Entomopathogenic Microorganisms in Pest Control
- Quantum Dots Synthesis And Properties
- Enzyme Production and Characterization
- Coagulation and Flocculation Studies
- Insect Pest Control Strategies
- Iron Metabolism and Disorders
- Chalcogenide Semiconductor Thin Films
- Carbohydrate Chemistry and Synthesis
- Nanocluster Synthesis and Applications
- Brazilian Legal Issues
- Pineapple and bromelain studies
- SARS-CoV-2 and COVID-19 Research
- Reservoir Engineering and Simulation Methods
- Nanoparticle-Based Drug Delivery
- Nanoparticles: synthesis and applications
- Proteoglycans and glycosaminoglycans research
Universidade Federal de São Paulo
2012-2024
Universidade de Brasília
2024
Rice University
2023
Hôpital Lapeyronie
2018
IBM Research - Brazil
2016
Universidade Federal de Pernambuco
2009-2013
Pontifical Catholic University of Rio de Janeiro
2009
École Normale Supérieure - PSL
2007
Aedes aegypti larvae have developed tolerance to many insecticides used for mosquito control. Moringa oleifera seeds contain a water-soluble lectin (WSMoL) and this paper reports the effect of M. seed extracts (MoE(1-15)) WSMoL on development survival A. larvae. peptide from in-gel trypsin digestion is also described. MoE(1-15) showed hemagglutinating activity had similarity with flocculating proteins seeds. MoE(1) MoE(3) delayed larval which stopped in third instar (L3) MoE(6) MoE(15)....
The aim of this work was to analyse the coagulant and antibacterial activities lectin isolated from Moringa oleifera seeds that are used for water treatment.The water-soluble M. (WSMoL) separated nonhemagglutinating components (NHC) by chitin chromatography. WSMoL fluorescence spectrum not altered in presence ions often present high concentrations polluted waters. Seed extract, NHC showed activity on a turbid model. Both reduced growth Staphylococcus aureus, but only caused reduction...
A new lectin, BfL, was purified from Bauhinia forficata seeds by ammonium sulfate fractionation, DEAE-Sephadex ion exchange chromatography, Sepharose-4B and chitin affinity chromatographies Superdex 75 size exclusion chromatography. The molecular homogeneity purity of BfL were assessed reversed-phase HPLC. appeared as a single band approximately 27.0 kDa on SDS-PAGE under non-reducing reducing conditions, its weight determined to be 27,850 Da LC/ESI-MS. is glycoprotein with carbohydrate...
Lectins are carbohydrate recognition proteins. cMoL, a coagulant Moringa oleifera Lectin, was isolated from seeds of the plant. Structural studies revealed heat-stable and pH resistant protein with 101 amino acids, 11.67 theoretical pI 81% similarity M. flocculent protein. Secondary structure content estimated as 46% α-helix, 12% β-sheets, 17% β-turns 25% unordered structures belonging to α/β tertiary class. cMoL significantly prolonged time required for blood coagulation, activated partial...
The kinetics of nanoparticle–protein interactions is modulated by nanoparticle surface chemistry.
The catalytic activity of enzymes can be regulated by interactions with synthetic nanoparticles (NPs) in a number ways. To date, however, the potential use NPs as allosteric effectors has not been investigated detail. Importantly, targeting (distal) sites on enzyme surface could afford unique ways to modulate activity, allowing for either activation, partial or full inhibition. Using p-mercaptobenzoic acid-coated ultrasmall gold (AuMBA) and human α-thrombin model system, here we...
A protein isolated from the bark of Crataeva tapia (CrataBL) is both a Kunitz-type plant protease inhibitor and lectin. We have determined amino acid sequence three-dimensional structure CrataBL, as well characterized its selected biochemical biological properties. found two different isoforms CrataBL original source, differing in positions 31 (Pro/Leu); 92 (Ser/Leu); 93 (Ile/Thr); 95 (Arg/Gly) 97 (Leu/Ser). showed relatively weak inhibitory activity against trypsin (Kiapp = 43 µM) was more...
ObjectiveTransforming growth factor-β (TGFβ) is a major regulator of cartilage homeostasis and its deregulation has been associated with osteoarthritis (OA). Deregulation the TGFβ pathway in mesenchymal stem cells (MSCs) proposed to be at onset OA. Using secretome analysis, we identified member family, TGFβ-induced protein (TGFβi or βIGH3), expressed MSCs investigated function regulation during OA.DesignCartilage, bone, synovium, infrapatellar fat pad bone marrow-MSCs were isolated from...
CrataBL, a protein isolated from Crataeva tapia bark, which is both serine protease inhibitor and lectin, has been previously shown to exhibit number of interesting biological properties, including anti-inflammatory, analgesic, antitumor, insecticidal activities. Using glycan array, we have now that only sulfated carbohydrates are effectively bound by CrataBL. Because this was recently delay clot formation impairing the intrinsic pathway coagulation cascade, considered its natural ligand...
Gold nanoparticles (NPs) in the ultrasmall size regime provide a new paradigm way that nanomaterials can be used to regulate protein structure and function. However, rational design of NPs as viable synthetic effectors function requires detailed quantitative understanding their biomolecular interactions. Herein, we focused on kinetics NP–protein complexation—an often neglected factor studies at bio-nanointerface—to gain molecular-level insights into mechanisms The α-thrombin gold coated with...
Two recombinant protease inhibitors from Bauhinia bauhinioides, rBbKI (kallikrein inhibitor) and rBbCI (cruzipain were evaluated for insecticidal activity against workers soldiers of Nasutitermes corniger (order: Isoptera; family: Termitidae) through the inhibitors' effect on insect's gut enzymes. The inhibitor was more effective than in inhibiting termite's kallikrein showed termiticidal with an LC50 0.9 mg mL-1 after 4 days. Conversely, did not affect survival show N. corniger. two...
Background. CrataBL is a protein isolated from Crataeva tapia bark. It has been shown to exhibit several biological properties, including anti-inflammatory, analgesic, antitumor, and insecticidal activities. There are no studies evaluating the role of in experimental asthma models. Aim. To evaluate effects on lung mechanics, inflammation, remodeling, oxidative stress activation mice with allergic pulmonary inflammation. Materials Methods. BALB/c (6-7 weeks old, 25-30g) were divided into four...
Currently available drugs for treatment of glioblastoma, the most aggressive brain tumor, remain inefficient, thus a plethora natural compounds have already been shown to antimalignant effects. However, these not tested their impact on tumor cells in microenvironment-simulated cell models, e.g., mesenchymal stem coculture with glioblastoma U87 (GB). Mesenchymal (MSC) chemotactically infiltrate microenvironment. Our previous studies that bone-marrow derived MSCs impair growth and invasion via...
Baupain belongs to the α+β class of proteins with a secondary structure-content 44% α-helix, 16% β-sheet and 12% β-turn. The structural transition induced by pH was found be noncooperative, no important differences observed in range from 3.0 10.5. At 2.0 protein presented substantial non-native structure strong ANS binding. Guanidine hydrochloride (GdnHCl)-induced unfolding did not change significantly until 4.0 M, indicating high rigidity molecule. cooperative, as seen sigmoidal curves...
Callosobruchus maculatus is an important predator of cowpeas. Due to infestation during storage, this insect affects the quality seed and crop yield. This study aimed investigate effects CrataBL, a multifunction protein isolated from Crataeva tapia bark, on C. larva development. The protein, which stable even in extreme pH conditions, showed toxic activity, reducing larval mass 45 70% at concentrations 0.25 1.0% (w/w), respectively. Acting as inhibitor, CrataBL decreased by 39% activity...
Nanomaterials displaying well-tailored sizes and surface chemistries can provide novel ways with which to modulate the structure function of enzymes. Recently, we showed that gold nanoparticles (AuNPs) in ultrasmall size regime could perform as allosteric effectors inducing partial inhibition thrombin activity. We now find nature AuNP chemistry controls interactions anion-binding exosites 1 2 on thrombin, allosterically induced changes active-site conformation, and, by extension, enzymatic...
Protease inhibitors are involved in the regulation of endogenous cysteine proteases during seed development and play a defensive role because their ability to inhibit exogenous such as those present digestive tracts insects. Araucaria angustifolia seeds, which can be used human animal feed, were investigated for potential agricultural biotechnology field health. In pine nuts extract, blocked activities proteases, it was detected potent insecticidal activity against termites (Nasutitermes...
Abstract Arterial thrombosis is an important complication of diabetes and cancer, being target for therapeutic intervention. Crataeva tapia bark lectin (CrataBL) has been previously shown to have hypoglycemiant effect also induce cancer cell apoptosis. It showed inhibitory activity against Factor Xa (K iapp =8.6 μ m ). In the present study, we evaluated anti-thrombotic properties CrataBL in arterial model. prolongs activated partial thromboplastin time on human mouse plasma, it impairs...
SARS-CoV2 virus, responsible for the COVID-19 pandemic, has four structural proteins and 16 nonstructural proteins. S-protein is one of exposed on virus surface main target producing neutralizing antibodies vaccines. The forms a trimer that can bind angiotensin-converting enzyme 2 (ACE2) through its receptor binding domain (RBD) cell entry.
Detecting salt domes in seismic images is very important for the exploration of petroleum reservoirs. However, this task characterized as being time-consuming when performed by human interpreters due to structural complexity bodies found on different volumes. This work aims at performing a comprehensive evaluation texture descriptors broadly used image processing community applied images. A robust multi-scale analysis conducted order assess which features and corresponding parameters are...