A5007936650- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Cell Adhesion Molecules Research
- RNA modifications and cancer
- Enzyme Structure and Function
- Monoclonal and Polyclonal Antibodies Research
- RNA Research and Splicing
- Toxin Mechanisms and Immunotoxins
- Signaling Pathways in Disease
- Platelet Disorders and Treatments
- Biochemical and Structural Characterization
- Galectins and Cancer Biology
- HIV/AIDS drug development and treatment
- Venomous Animal Envenomation and Studies
- Electrocatalysts for Energy Conversion
- HIV Research and Treatment
- Cancer Research and Treatments
- Magnesium Alloys: Properties and Applications
- Climate change and permafrost
- Aluminum Alloys Composites Properties
- Polyamine Metabolism and Applications
- Amino Acid Enzymes and Metabolism
- Protease and Inhibitor Mechanisms
- RNA regulation and disease
- Connective tissue disorders research
Nagoya University
2023
Jilin University
2019
Versiti Blood Center of Wisconsin
2015-2018
National Cancer Institute
2011-2017
Center for Cancer Research
2011-2016
National Institutes of Health
2011-2015
Frederick Community College
2014
Frederick National Laboratory for Cancer Research
2011-2013
Beijing University of Technology
2011
Chinese Academy of Sciences
2006-2008
We introduced a nomenclature for split inteins and engineered mini-inteins. 21 new were created based on the four intein structures.<italic>trans</italic>-Splicing cross-activity of 24 systematically compared. >75% active >90% combinations orthogonal.
The individual synthesis of methanol and ethane from methane was investigated using a thermo-electrochemical cell in gas flow mode over the temperature range 150–200 °C. Methane directly oxidized at an anode consisting sub-10 nm Pt Fe particles. In electrolysis humidified methane, produced through formation active oxygen intermediates water vapor. unhumidified via dissociation C–H bonds, followed by dimerization resultant •CH3 radicals. However, rates for target products decreased with time...
A serine protease inhibitor from Enterolobium contortisiliquum (EcTI) belongs to the Kunitz family of plant inhibitors, common in seeds. It was shown that EcTI inhibits invasion gastric cancer cells through alterations integrin-dependent cell signaling pathway. We determined high-resolution crystal structures free (at 1.75 Å) and complexed with bovine trypsin 2 Å). High quality resulting electron density maps redundancy structural information indicated sequence crystallized isoform contained...
A protein isolated from the bark of Crataeva tapia (CrataBL) is both a Kunitz-type plant protease inhibitor and lectin. We have determined amino acid sequence three-dimensional structure CrataBL, as well characterized its selected biochemical biological properties. found two different isoforms CrataBL original source, differing in positions 31 (Pro/Leu); 92 (Ser/Leu); 93 (Ile/Thr); 95 (Arg/Gly) 97 (Leu/Ser). showed relatively weak inhibitory activity against trypsin (Kiapp = 43 µM) was more...
CrataBL, a protein isolated from Crataeva tapia bark, which is both serine protease inhibitor and lectin, has been previously shown to exhibit number of interesting biological properties, including anti-inflammatory, analgesic, antitumor, insecticidal activities. Using glycan array, we have now that only sulfated carbohydrates are effectively bound by CrataBL. Because this was recently delay clot formation impairing the intrinsic pathway coagulation cascade, considered its natural ligand...
Integrin α/β heterodimer adopts a compact bent conformation in the resting state, and upon activation undergoes large-scale conformational rearrangement. During inside-out activation, signals impinging on cytoplasmic tail of β subunit induce separation at transmembrane domains, leading to extended ectodomain with separated leg opening headpiece that is required for high-affinity ligand binding. It remains enigmatic which integrin drives bent-to-extended rearrangement activation. The 3...
In most eukaryotes, poly(A) tails are attached to the 3′-ends of mRNAs. They influence almost all aspects mRNA metabolism: transport from nuclear cytoplasm, translation, and turnover. To mediate its biological functions, covered in sequence-specific manner by two types conserved binding proteins, PABPC cytoplasm PABPN1 nucleus. (Pab1p Saccharomyces cerevisiae1) is present eukaryotes. It plays important roles initiation translation2 decay.3 contains four copies RRMs [the RNA recognition...
Lectins are a group of proteins that recognize carbohydrates covalently linked to and lipids on the cell surface within extracellular matrix have diverse physiological functions1-3 including growth regulation,4-6 adhesion,7, 8 pre-mRNA splicing,9, 10 migration,11, 12 apoptosis,13-15 immune responses,16 pathogen recognition.17 The galectins family animal lectins defined by their shared conserved carbohydrate recognition domain (CRD) about 130 amino acids affinity for β-galactoside sugars.18,...
A serine protease inhibitor from Bauhinia bauhinioides (BbKI) belongs to the Kunitz family of plant inhibitors, which are common in seeds. BbKI does not contain any disulfides, unlike most other members this family. It is a potent plasma kallikrein, addition proteases, and thus exhibits antithrombotic activity. high-resolution crystal structure recombinantly expressed was determined (at 1.4 Å resolution) compared with structures Modeling complex kallikrein indicates that changes local...
Background We applied crosslinking techniques as a first step in preparation of stable avian sarcoma virus (ASV) integrase (IN)-DNA complexes for crystallographic investigations. These results were then compared with the crystal structures prototype foamy (PFV) intasome and published data other retroviral IN proteins. Methodology/Results Photoaffinity site-directed chemical used to localize sites contacts DNA substrates on surface ASV IN. Sulfhydryl groups cysteines engineered into...
Galectins are a family of animal lectins which share similar carbohydrate-recognition domains (CRDs) and an affinity for β-galactosides. A novel human galectin-related protein named GRP (galectin-related protein; previously known as HSPC159) comprises only one conserved CRD with 38 additional N-terminal residues. The C-terminal fragment (GRP-C; residues 38–172) containing the has been expressed purified. was crystallized using hanging-drop vapour-diffusion method from solution 2% PEG 400 2M...
The RadA intein from the hyperthermophilic archaebacterium Pyrococcus horikoshii was cloned, expressed and purified for subsequent structure determination. protein crystallized rapidly in several conditions. best crystals, which diffracted to 1.75 Å resolution, were harvested drops consisting of 0.1 M HEPES pH 7.5, 3.0 NaCl cryoprotected with Paratone-N before flash-cooling. collected data processed orthorhombic space group P212121, unit-cell parameters a = 58.1, b 67.4, c 82.9 Å. Molecular...
Histamine dehydrogenase (HADH) catalyzes the oxidative deamination of histamine, resulting in production imidazole acetaldehyde and an ammonium ion. The enzyme isolated from newly identified halophilic archaeon Natrinema gari BCC 24369 is significantly different previously described protein Nocardioides simplex. This HADH comprises three subunits with molecular weights 49.0, 24.7 23.9 kDa, respectively, optimally active under high-salt conditions (3.5-5 M NaCl). As a step exploration unique...