Antibiotic resistance is at dangerous levels and increasing worldwide. The search for new antimicrobial drugs to counteract this problem a priority health institutions organizations, both globally in individual countries. Sarconesiopsis magellanica blowfly larval excretions secretions (ES) are an important source isolating peptides (AMPs). This study aims identify characterize S. AMP. RP-HPLC was used fractionate ES, using C18 columns, their activity evaluated. peptide sequence of the...

Objective: To analyze the need to consolidate governance systems in Federal Universities, highlighting leadership as an essential pillar and using University of Sergipe (UFS) a reference. Theoretical Framework: The research is grounded concepts public described Decree No. 9,203/2017 guidelines Court Accounts (TCU). Leadership emphasized central element. Within context federal universities, study examines impact on interaction with institutional autonomy, while addressing challenges...

This study aimed to compare different protocols (Protocol 1: P1; Protocol 2: P2; 3: P3; 4: P4) for the extraction of spongin-like collagen (SC) from marine sponges. The SEM micrographs demonstrated a fibrillar structure extracts Chondrilla caribensis and nodular/particulate aggregates Aplysina fulva. FTIR showed all samples peaks similar both species. For C. caribensis, obtained using P2, P3, P4 presented higher values yield, TPQ, GAGs. P2 SC concentration antioxidant analysis. A. fulva,...

Angiotensin II (AII) as well analog peptides shows antimalarial activity against Plasmodium gallinaceum and falciparum , but the exact mechanism of action is still unknown. This work presents solid‐phase synthesis characterization eight corresponding to alanine scanning series AII plus amide‐capped derivative evaluation antiplasmodial these mature P. sporozoites. The Ala screening data indicates that replacement either Ile 5 or His 6 residues causes minor effects on in vitro compared with...

Abstract Malaria can have severe long-term effects. Even after treatment with antimalarial drugs eliminates the parasite, survivors of cerebral malaria may suffer from irreversible brain damage, leading to cognitive deficits. Angiotensin II, a natural human peptide hormone that regulates blood pressure, has been shown be active against Plasmodium spp., etiologic agent malaria. Here, we tested two Ang II derivatives do not elicit vasoconstriction in mice: VIPF, linear tetrapeptide, which...

Abstract Angiotensin II (Ang II) is a natural mammalian hormone that has been described to exhibit antiplasmodial activity therefore constituting promising alternative for the treatment of malaria. Despite its promise, development Ang as an antimalarial limited by potent induction vasoconstriction and rapid degradation within minutes. Here, we used peptide design perform targeted chemical modifications generate conformationally restricted (disulfide-crosslinked) derivatives with suppressed...

One of the most important cellular events in arthropods is moulting cuticle (ecdysis). This process allows them to grow until they reach sexual maturity. Nevertheless, during this stage, animals are highly exposed pathogens. Consequently, it can be assumed that counter with an efficient anti-infective strategy facilitates their survival ecdysis. Herein, we characterized a novel antimicrobial peptide called Pinipesin, present exuviae extract centipede Scolopendra subspinipes subspinipes. The...

Malaria is an infectious disease responsible for approximately one million deaths annually. The antimalarial effects of angiotensin II and its analogs against Plasmodium gallinaceum P. falciparum have recently been reported. To evaluate antiplasmodial activity, we synthesized five II-restricted containing disulfide bridges. accomplish this, peptides two inserted amino acid residues (cysteine) were by the Fmoc solid-phase method, purified liquid chromatography, characterized mass...

Protease inhibitors are involved in the regulation of endogenous cysteine proteases during seed development and play a defensive role because their ability to inhibit exogenous such as those present digestive tracts insects. Araucaria angustifolia seeds, which can be used human animal feed, were investigated for potential agricultural biotechnology field health. In pine nuts extract, blocked activities proteases, it was detected potent insecticidal activity against termites (Nasutitermes...

Abstract IsCT1‐NH 2 is a cationic antimicrobial peptide isolated from the venom of scorpion Opisthacanthus madagascariensis that has tendency to form an α‐helical structure and shows potent activity also inopportunely hemolytic effects. In this study, five IsCT1 (ILGKIWEGIKSLF)‐based analogs with amino acid modifications at positions 1, 3, 5, or 8 one analog three simultaneous substitutions were designed. The net charge each was between +2 +3. peptides obtained characterized by mass...

Antiplasmodial activities of angiotensin II and its analogues have been extensively investigated in Plasmodium gallinaceum falciparum parasite species. Due to vasoconstrictor property cannot be used as an anti-malarial drug. This work presents the solid-phase syntheses liquid chromatography mass spectrometry characterization ten linear peptides related against mature P. sporozoites erythrocyte invasion by falciparum. Conformational analyses were performed circular dichroism. IC50 assays...

Malaria is an infectious disease responsible for approximately one million deaths annually. Oligopeptides such as angiotensin II (AII) and its analogs are known to have antimalarial effects against Plasmodium gallinaceum falciparum. However, their mechanism of action still not fully understood at the molecular level. In work reported here, we investigated this issue by comparing activity AII with that (i) diastereomer formed only d-amino acids; (ii) isomer reversed sequence; (iii) restricted...

In several Apicomplexa, the formation of moving junctions (MJs) at interface between external membranes invading parasite and host cell is essential for process invasion. Plasmodium falciparum Toxoplasma gondii, MJ composed Apical Membrane Antigen 1 (AMA1) Rhoptry Neck Proteins (RONs) complex; specifically, AMA1 interacts with RON2 during invasion.Recombinant proteins based on vivax (A2033-P2100) its synthetic peptide fragments, one cyclic linear, PvRON2 (D2035-T2074) were generated used to...

Proteinase inhibitors extracted form medicinal plants are an interesting source of new drugs. Modifications in the structure some this kind macromolecules could also lead to compounds biological properties. In work, we synthesized and tested one fragment containing reactive site Bauhinia bauhinioides kallikrein inhibitor (BbKI), denoted BbKI51–62, a related analog (P2) which proline residue was inserted order mimic N-terminal region bradykinin molecule. The retro-inverso counterparts...

Araucaria angustifolia seeds are characterised by a relatively high content of starch and protein. This study aimed to verify the presence α-amylase inhibitors in characterise trypsin inhibitor found embryo tissues. Inhibitor purification was carried out saline extraction proteins, acetone precipitation affinity chromatography. Two protein bands molecular weight estimated SDS-PAGE at about 35 kDa were further examined high-performance liquid chromatography coupled mass spectrometer shown be...

Angiotensin II (AngII) is the final active product of renin enzymatic cascade, which responsible for sustaining blood pressure. To investigate effect N-terminal cyclization on AT1 activation and tachyphylaxis, we designed conformationally constrained analogues with an i-(i + 1) lactam bridge. All presented same binding coefficient tachyphylactic index, but some them such as Cyclo (0-1a) [Glu0 , endo-(Lys1a )]-AngII [Asp0 endo-(Orn1a showed higher potency. The index by AngII cyclic was...

Antimicrobial peptides and proteins (AMPs) are molecules that can interact with microbial cells lead to membrane disruption or intracellular molecule interactions death. Several antimicrobial effects also present other biological activities. One such protein group representing the duplicity of activities is tachykinin family. Tachykinins (TKs) form a family neuropeptides in vertebrates consensus C-terminal region (F-X-G-Y-R-NH2). Invertebrate TKs TK-related (TKRPs) subfamilies found...

Head and neck squamous cell carcinoma (HNSCC) represents a serious health problem worldwide. Approximately 75% of patients with HNSCC have locally advanced disease at diagnosis, the therapy for those cases involves chemoradiation or induction chemotherapy, in which cisplatin is included despite its substantial side effects. FAPESP-founded "Cancer Innovation Center Emphasis on Metals Theranostics" (CancerThera) dedicated to development new metallopharmaceuticals radiopharmaceuticals tumor...

Peptides are biomolecules that have been associated with various physiological responses and great potential for the diagnosis or treatment of diseases, including types tumors. Several studies shown biologically active peptides containing RGD domain a high affinity αvβ3 integrin adhesion molecule overexpressed in tumor cells, assisting molecular imaging targeted radionuclide therapy (TRT) as an anti-tumor agent. To obtain anti-integrin peptide fragment modified by incorporating two spacers —...