A5027533013- Venomous Animal Envenomation and Studies
- Rabies epidemiology and control
- Amphibian and Reptile Biology
- Marine Invertebrate Physiology and Ecology
- Healthcare and Venom Research
- Blood Coagulation and Thrombosis Mechanisms
- Mosquito-borne diseases and control
- Insect and Pesticide Research
- Turtle Biology and Conservation
- Insect Resistance and Genetics
- Vector-borne infectious diseases
- Research on Leishmaniasis Studies
- Insect symbiosis and bacterial influences
- Invertebrate Immune Response Mechanisms
- Antimicrobial Peptides and Activities
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Biochemical and Structural Characterization
- Neurobiology and Insect Physiology Research
- Nicotinic Acetylcholine Receptors Study
- Calpain Protease Function and Regulation
- Yersinia bacterium, plague, ectoparasites research
- Hemophilia Treatment and Research
- S100 Proteins and Annexins
- Streptococcal Infections and Treatments
- Blood properties and coagulation
Instituto Butantan
2015-2025
Instituto de Pesquisas Tecnológicas
2017-2025
Universidade de São Paulo
2013-2025
Universidade Federal de Minas Gerais
2020
Universidade Estadual Paulista (Unesp)
2020
Museu de Ciências Naturais da Fundação Zoobotânica do Rio Grande do Sul
2020
Universidade Federal de São Paulo
2020
Snakebite is a globally neglected tropical disease, with coagulation disturbances being the primary pathology of many deadly snake venoms. Age-related differences in human plasma have been abundantly reported, yet effect that these pose regarding snakebite largely unknown. We tested for coagulotoxic effects (via clotting time) multiple venoms upon healthy adult (18+) and paediatric (median 3.3 years old) vivo compared to time it takes plasmas clot without addition venom (the spontaneous...
Antivenoms are the only effective treatment for snakebite envenomation and have saved countless lives over more than a century. Despite their value, antivenoms present risks of adverse reactions. Current formulations contain fraction nonspecific antibodies serum proteins. While new promising candidates emerge as next generation antivenoms, it remains clear that animal-derived will still play critical role years to come. In this study, we improved bothropic antivenom (BAv), by capturing...
Recently, we have cloned several Kazal-type serine protease inhibitors from the midgut of Triatoma infestans bug. A single gene composed multi domains, in tandem, encodes these inhibitors. In this work, describe purification and characterization recombinant infestins 3-4 4, which are potent factor XIIa (KI=67 pM 128 pM, respectively). We also identified first native inhibitor a hematophagous insect. The inhibitory activity infestin 4 demonstrates extremely efficient anticoagulant activity,...
Kazal-type inhibitors play several important roles in invertebrates, such as anticoagulant, vasodilator and antimicrobial activities. Putative were described insect transcriptomes. In this paper we characterized for the first time a Kazal unique domain trypsin inhibitor from Aedes aegypti mosquito. Previously, analyses of sialotranscriptome A. showed potential presence serine protease inhibitor, female salivary glands, carcass also whole male, which named AaTI (A. inhibitor). sequence amino...
Rhipicephalus (Boophilus) microplus is an ectoparasite responsible for important decrease in meat, milk and leather production, caused both by cattle blood loss the transmission of anaplasmosis babesiosis. R. a rich source serine protease inhibitors, including trypsin inhibitors BmTI-A BmTI-6, subtilisin inhibitor BmSI, recently described thrombin inhibitor, boophilin. Boophilin double Kunitz-type with unusual ability to form ternary complex second (non-thrombin) proteinase molecule. The...
Crotalus durissus is considered one of the most important species venomous snakes in Brazil, due to high mortality its snakebites. The venom contains four main toxins: crotoxin, convulxin, gyroxin and crotamine. Venoms can vary their crotamine content, being crotamine-negative or -positive. This heterogeneity great importance for producing antivenom, different mechanisms action. possibility that antivenom produced by Butantan Institute might have a immunorecognition capacity between...
Background: Variability in snake venoms is a well-studied phenomenon. However, sex-based variation of Bothrops atrox venom using siblings poorly investigated. responsible for the majority snakebite accidents Brazilian Amazon region. Differences composition genus have been linked to several factors such as ontogeny, geographical distribution, prey preferences and sex. Thus, current study, samples male female were analyzed order compare their biochemical biological characteristics. Methods:...
Despite coagulotoxicity being a primary weapon for prey capture by Bothrops species (lancehead pit vipers) and coagulopathy major lethal clinical effect, genus-wide comparison has not been undertaken. To fill this knowledge gap, we used thromboelastography to compare 37 venoms, from across the full range of geography, taxonomy, ecology, their action upon whole plasma isolated fibrinogen. Potent procoagulant toxicity was shown be main venom effect most tested. However, basal (B. pictus)...
Chagas disease, or American trypanosomiasis, is a parasitic disease caused by the protozoan Trypanosoma cruzi and transmitted insects from Triatominae subfamily. To identify components involved in protozoan-vector relationship, we constructed analyzed cDNA libraries RNA isolated midguts of uninfected T. cruzi-infected Triatoma infestans, which are major vectors disease. We generated approximately 440 high-quality Expressed Sequence Tags (ESTs) each infestans midgut library. The sequences...
The Brazilian lancehead (Bothrops moojeni) has a wide distribution in Brazil and represents serious public health hazard. Previous works reported that the symptoms of snakebites caused by B. moojeni juveniles' bites were mainly related to coagulation, while those adults' had more prominent local damage. In this work, we analyzed venoms at different life stages better understand ontogeny shift species. Snakes grouped age sex, venom pools formed accordingly. Compositional analyses...
Saliva of blood-sucking arthropods contains a complex mixture anti-haemostatic, anti-inflammatory and immune-modulator compounds. Among anti-haemostatic factors, there are anticoagulants, vasodilators platelet aggregation inhibitors. Previous analyses the sialotranscriptome Aedes aegypti showed potential presence Kazal-type serine protease inhibitor in female salivary glands, carcass also whole male, which we named AaTI (A. thrombin inhibitor). Recently, expressed characterized rAaTI as...
Blood coagulation is an important process in haemostasis, and disorders of blood can lead to increased risk haemorrhage thrombosis. Coagulation highly conserved mammals has been comprehensively studied humans the investigation bleeding or thrombotic diseases. Some substances act as inhibitors may affect one multiple enzymes throughout process. A specific thrombin inhibitor called infestin isolated from midgut haematophagous insect Triatoma infestans. Infestin a member nonclassical Kazal-type...
Phospholipases A2 (PLA2) are enzymes acting on the cell membrane phospholipids resulting in fatty acids and lysophospholipids deconstructing membrane. This protein is commonly found snake venoms, causing tissue inflammation affected area. Evidence indicates that snakes have natural resistance to their own venom due protective properties plasma, inhibit action of proteins present venom. Given that, this study aimed purify characterize a γPLI from Bothrops jararaca serum, named γBjPLI. PLA2...
South American rattlesnakes are represented in Brazil by a single species, Crotalus durissus, which has public health importance due to the severity of its envenomation and wide geographical distribution. The species is subdivided into several subspecies, but current classification controversial. In Brazil, venoms C. d. terrificus collilineatus used for hyperimmunization horses antivenom production, even though distinction these two subspecies mostly their this context, we described...