A5029707662- Aquaculture Nutrition and Growth
- Venomous Animal Envenomation and Studies
- Trypanosoma species research and implications
- Aquaculture disease management and microbiota
- Protein Hydrolysis and Bioactive Peptides
- Insect symbiosis and bacterial influences
- Research on Leishmaniasis Studies
- Turtle Biology and Conservation
- Invertebrate Immune Response Mechanisms
- Amphibian and Reptile Biology
- Helminth infection and control
- Enzyme Production and Characterization
- Insect Utilization and Effects
- Iron Metabolism and Disorders
- Dye analysis and toxicity
- Fish Biology and Ecology Studies
- Antioxidant Activity and Oxidative Stress
- Mosquito-borne diseases and control
- Essential Oils and Antimicrobial Activity
- Microplastics and Plastic Pollution
- Enzyme-mediated dye degradation
- Neurobiology and Insect Physiology Research
- Phytochemicals and Antioxidant Activities
- Aquatic life and conservation
- Plant biochemistry and biosynthesis
Universidade Federal Rural de Pernambuco
2018-2023
Universidade Federal de Pernambuco
2008-2020
Universidade Federal de São Paulo
2010-2017
Biologie Labor
2009
Chagas disease, or American trypanosomiasis, is a parasitic disease caused by the protozoan Trypanosoma cruzi and transmitted insects from Triatominae subfamily. To identify components involved in protozoan-vector relationship, we constructed analyzed cDNA libraries RNA isolated midguts of uninfected T. cruzi-infected Triatoma infestans, which are major vectors disease. We generated approximately 440 high-quality Expressed Sequence Tags (ESTs) each infestans midgut library. The sequences...
Proteases from the midgut gland of Farfantepenaeus paulensis juveniles were assessed. Enzyme activity was determined using protease substrates and inhibitors. The effect pH, temperature calcium on proteolytic assayed. Caseinolytic analysed in substrate-sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Trypsin, chymotrypsin leucine aminopeptidase detected. Proteolytic strongly inhibited by specific trypsin Tosyl-phenylalanine chloromethyl ketone 59.3% activity. greatest...
The aim of this study was to examine proteinases and peptidases from the hepatopancreas two sub-adult stages Farfantepenaeus subtilis: SAS6 (5.93 ± 0.69 g wet weight) SAS13 (13.26 0.60 weight). Trypsin chymotrypsin activity higher in extract individuals (P < 0.05). highest among aminoacyl-β-naphthylamide substrates found using alanine-, arginine-, leucine- lysine-β-naphthylamide. There a positive correlation between recommended concentration essential amino acids penaeid shrimp feed...
Pacifastin-like protease inhibitors belong to a recent classified inhibitor family and they are the smallest described in animals. In this work, we purified characterized, for first time, two neutrophil elastase belonging pacifastin from blood sucking insect Triatoma infestans eggs. The showed same N-terminal sequences, molecular masses of 4257 4024Da by MALDI-TOF mass spectrometry dissociation constants (Ki) 0.52 0.29nM, respectively. Using fat body cDNA library, cloned precursor containing...
The insect Triatoma infestans is a vector of Trypanosoma cruzi, the etiological agent Chagas disease. A cDNA library was constructed from T. anterior midgut, and 244 clones were sequenced. Among EST sequences, an open reading frame (ORF) with homology to cystatin type 2 precursor identified. Then, 288-bp fragment encoding mature (lacking signal peptide) named Tigutcystatin cloned fused N-terminal His tag in pET-14b vector, protein expressed Escherichia coli strain Rosetta gami. purified...
Background Snakes belonging to the Bothrops genus are vastly distributed in Central and South America responsible for most cases of reported snake bites Latin America. The clinical manifestations envenomation caused by this due three major activities—proteolytic, hemorrhagic coagulant—mediated metalloproteinases, serine proteinases, phospholipases A 2 other toxic compounds present venom. Interestingly, it was observed that snakes resistant effects its own snake’s venoms. This natural...