A5079505587- Cystic Fibrosis Research Advances
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Infections and bacterial resistance
- Plant Pathogenic Bacteria Studies
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Mycobacterium research and diagnosis
- Vibrio bacteria research studies
- Tracheal and airway disorders
- Infective Endocarditis Diagnosis and Management
- Enterobacteriaceae and Cronobacter Research
- Tuberculosis Research and Epidemiology
- Inhalation and Respiratory Drug Delivery
- Diphtheria, Corynebacterium, and Tetanus
- Nematode management and characterization studies
- Streptococcal Infections and Treatments
- Public Health in Brazil
- Immune Response and Inflammation
- Genomics and Phylogenetic Studies
- Healthcare Regulation
- Sepsis Diagnosis and Treatment
- Gut microbiota and health
- Actinomycetales infections and treatment
- Microbial Applications in Construction Materials
Universidade do Estado do Rio de Janeiro
2015-2025
Hospital Universitário Pedro Ernesto
2010-2025
Instituto Nacional de Saúde
2019
Fundação Oswaldo Cruz
2011
An epidemic of infections after video-assisted surgery (1,051 possible cases) caused by rapidly growing mycobacteria (RGM) and involving 63 hospitals in the state Rio de Janeiro, Brazil, occurred between August 2006 July 2007. One hundred ninety-seven cases were confirmed positive acid-fast staining and/or culture techniques. Thirty-eight had mycobacterial culture, with a total 148 available isolates recovered from 146 patients. Most (n = 144; 97.2%) presented PRA-hsp65 restriction pattern...
The most important resistance mechanism against β-lactam drugs is the production of carbapenemases. In this study, we report first identification Klebsiella pneumoniae carbapenemase (KPC)-2 and New Delhi metallo-β-lactamase (NDM)-1 in Enterobacter hormaechei subps. oharae from Brazil. detection carbapenemases was done by phenotypic assays, PCR, DNA sequencing, whereas performed conventional techniques, sequencing 16S rDNA gene, hsp60-genotyping. Molecular typing using pulsed-field gel...
As Pseudomonas aeruginosa ExoU possesses two functional blocks of homology to calcium-independent (iPLA2) and cytosolic phospholipase A2 (cPLA2), we addressed the question whether it would exhibit a proinflammatory activity by enhancing synthesis eicosanoids host organisms. Endothelial cells from HMEC-1 line infected with ExoU-producing PA103 strain exhibited potent release arachidonic acid (AA) that could be significantly inhibited methyl arachidonyl fluorophosphonate (MAFP), specific PLA2...
The present study was designed to evaluate the molecular epidemiology of CTX-M producing Klebsiella pneumoniae, Enterobacter cloacae and Escherichia coli isolated from bloodstream infections at tertiary care hospitals in State Rio de Janeiro, Brazil.A total 231 nonduplicate Enterobacteriaceae were five Brazilian between September 2007 2008. antimicrobial susceptibility testing performed by disk diffusion method according Clinical Laboratory Standard Institute. Isolates showing resistance...
We investigated the possibility of cross-infection among cystic fibrosis patients in two Brazilian reference centers. Achromobacter xylosoxidans isolates (n = 122) were recovered over a 5-year period from 39 patients. Isolates genetically heterogeneous, but one genotype was present 56% patients, suggesting that may have occurred.
ABSTRACT Worldwide, nontuberculous mycobacteria (NTM) have become emergent pathogens of pulmonary infections in cystic fibrosis (CF) patients, with an estimated prevalence ranging from 5 to 20%. This work investigated the presence NTM sputum samples 129 CF patients (2 18 years old) submitted longitudinal clinical supervision at a regional reference center Rio de Janeiro, Brazil. From June 2009 March 2012, 36 isolates recovered 10 (7.75%) out children were obtained. Molecular identification...
Pulmonary deterioration after B.cepacia complex (BCC) colonization has a heterogeneous pattern. The aim was to investigate the clinical outcome of BCC in CF patients chronically colonized with P. aeruginosa.CF aeruginosa were divided into three groups: intermittent (I), chronic (II) and no (III) BCC. Body mass index (BMI) percentile spirometric parameters analyzed at different times each group.Fifty-six included. Of these, 27 also had evidence (13 14 chronic). BMI significantly lower among...
The rate of diagnosis colonization/infection the airways with Achromobacter xylosoxidans has increased in cystic fibrosis patients, but its clinical significance is still controversial. This retrospective, case-control study aimed to evaluate impact A. patients. Individuals who were chronically colonized/infected (n=10), intermittently (n=15), and never (n=18) retrospectively evaluated during two periods that 2 years apart. Demographic characteristics, data, lung function, chronic bacterial...
Summary The tracheobronchial secretions from patients with cystic fibrosis often contain high amounts of free proteases. To evaluate whether human leucocyte elastase (HLE) can favour the persistence bacterial airways infection, we exposed frog palate mucosa to HLE and then radiolabelled Pseudomonas aeruginosa followed sequence events by scanning electronmicroscopy. In response there was a marked outpouring mucus desquamation epithelium. P. shown adhere recently secreted granules submucosal...
To examine the occurrence of and to determine antimicrobial susceptibility Corynebacterium pseudodiphtheriticum among patients with bacterial infections at a teaching hospital.A total 113 Coryne. strains identified by conventional biochemical methods API-Coryne System were recovered from different age groups: 65.48% adults (18 < or =59 years old), 9.73% aged (> =60 old); 14.15% infants (<18 4.42% newborns (0-7 days). Micro-organisms mostly related in urinary (29.2%) respiratory tracts...
PURPOSE: To evaluate the minimum inhibitory concentration (MIC) of GTA against these microorganisms and alternative disinfectants for high-level disinfection (HLD). METHODS: Reference mycobacteria clinical M. massiliense strains were included in this study. Active cultures submitted to susceptibility qualitative tests with dilutions (ranging from 1.5% 8%), commercial orthophthaldehyde (OPA) peracetic acid (PA) - based solutions, during period exposure as recommended by National Agency...
SUMMARY Achromobacter spp. are opportunistic pathogens increasingly recovered from adult patients with cystic fibrosis (CF). We report the characterization of 122 isolates 39 CF by multilocus sequence typing, virulence traits, and susceptibility to antimicrobials. Two species, A. xylosoxidans (77%) ruhlandii (23%) were identified. All showed a similar biofilm formation ability, positive swimming phenotype. By contrast, 4·3% 44·4% , respectively, exhibited negative swarming phenotype, making...
Background Multidrug resistance is a critical factor in tuberculosis control. To gain better understanding of multidrug resistant Brazil, retrospective study was performed to compare genotypic diversity and drug associated mutations Mycobacterium isolates from national reference center. Methods Findings Ninety-nine 12 Brazilian states were studied. Drug-resistance patterns determined the rpoB katG genes screened for mutations. Genotypic investigated by IS6110-RFLP Luminex 47 spoligotyping....
Molecular methodologies were used to identify 28 Achromobacter spp. from patients with cystic fibrosis (CF). Multilocus sequence typing (MLST) identified 17 xylosoxidans isolates (all bla(OXA-114) positive), nine ruhlandii one dolens isolate, and insuavis isolate. All less common species misidentified as A. by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Chronic colonization clonally related was demonstrated.
Over a five-year period, 163 strains of Corynebacterium sp. were recovered from different clinical specimens patients Brazilian University hospital. Genitourinary tract and intravenous sites the most frequent sources corynebacteria (46.62%). amycolatum (29.55%), minutissimum (20.45%) pseudodiphtheriticum (13.63%) predominant species found in genitourinary tract. C. (24.14%) propinquum (17.24%) surgical and/or other skin wounds abscesses; xerosis (25%), (21.87%) (18.75%) sites; (33.33%) lower...
To investigate phenotypic aspects including biotyping, drug susceptibility and production of extracellular enzymes genetic diversity Stenotrophomonas maltophilia clinical strains obtained from seven hospitals in Rio de Janeiro, Brazil.Thirty-nine S. were investigated by biotying, testing, detection randomly amplified polymorphic DNA (RAPD)-PCR. Biotyping distinguished 13 biotypes among 39, one them was prevalent. The majority the produced DNase, gelatinase haemolysin. Protease, lipases...
Knowledge about the virulence mechanisms of species from Burkholderia cepacia complex (BCC) is still limited. The genomovar heterogeneity and production different factors are likely to contribute variation in clinical outcome observed BCC-infected cystic fibrosis (CF) patients. Therefore, this study we investigated genetic polimorphism, presence makers associated with transmissibility BCC, profile exoenzyme 59 BCC isolates obtained CF patients attending reference centre Rio de Janeiro,...