A5030869812- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Enterobacteriaceae and Cronobacter Research
- Bacterial Identification and Susceptibility Testing
- Pneumonia and Respiratory Infections
- Escherichia coli research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotic Use and Resistance
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Bacterial biofilms and quorum sensing
- Pneumocystis jirovecii pneumonia detection and treatment
- Evolution and Genetic Dynamics
- Genomics and Phylogenetic Studies
- Tuberculosis Research and Epidemiology
- Infections and bacterial resistance
- Urinary Tract Infections Management
- Antimicrobial Peptides and Activities
- Antimicrobial Resistance in Staphylococcus
- Plant Pathogenic Bacteria Studies
- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- Clostridium difficile and Clostridium perfringens research
- Mycobacterium research and diagnosis
University Hospital of North Norway
2016-2025
UiT The Arctic University of Norway
2015-2024
Centre for Arctic Gas Hydrate, Environment and Climate
2024
CNR de la Résistance aux Antibiotiques
2019
Tromsø research foundation
2019
Telemark Hospital
2011
University of Bristol
2008-2009
Uppsala University
2006
University of Oslo
2006
Extraintestinal pathogenic Escherichia coli (ExPEC) is the main cause of urinary tract infections and septicemia. Significant attention has been given to ExPEC sequence type ST131, which categorized as a “high-risk” clone. High-risk clones are globally distributed associated with various antimicrobial resistance determinants, ease transmission, persistence in hosts, effective transmission between hosts. The high-risk have enhanced pathogenicity severe and/or recurrent infections. We show...
The class A carbapenemase KPC has disseminated rapidly worldwide, challenging the treatment of Gram-negative infections. This report describes first KPC-producing Klebsiella pneumoniae isolates identified in Norway (n=6) and second isolate from Sweden.Antimicrobial susceptibility profiles were determined using Etest. PCR sequencing used to determine bla(KPC) variant, surrounding genetic structure presence AmpC extended-spectrum beta-lactamase genes. PFGE multilocus sequence typing (MLST) for...
ABSTRACT Scandinavia is considered a region with low prevalence of antimicrobial resistance. However, the number multidrug-resistant (MDR) Gram-negative bacteria increasing, including metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa . In this study MBL-producing P. isolates identified in Norway ( n = 4) and Sweden 9) from 1999 to 2007 were characterized. Two international clonal complexes (CC), CC111 8) CC235 2), previously associated isolates, dominant. (ST111/229; serotype O12;...
The study investigated the species distribution, antibiotic susceptibility patterns and genotypic resistance characteristics of 113 consecutive blood culture isolates Acinetobacter collected between 2005 2007 throughout Norway. Species identification was performed by partial rpoB sequence analysis, verified 16S rDNA recA analyses. Susceptibility testing agar disc diffusion Etest. Distribution OXA carbapenemase genes epidemic clonality baumannii were detected PCR assays. Analyses...
Significance In many clinically important bacteria, antibiotic resistance genes are primarily carried on plasmids. These can spread horizontally between different strains and species. However, current surveillance systems track chromosomal lineages of bacteria only, leading to an incomplete understanding how spreads, from within individual hospital across country borders. We present integrated, high-resolution analysis both chromosome plasmid sequences using Klebsiella pneumoniae isolates...
To study the molecular characteristics of a long-term, low frequency outbreak bla KPC-2 in prevalence setting involving hospital environment.KPC-producing bacteria were screened by selective chromogenic agar and Real-Time PCR. The presence antibiotic resistance genes was ascribed PCRs subsequent sequencing, KPC-producing isolates phylogenetically typed using PFGE multi-locus sequence typing. Bla KPC-2-plasmids identified analysed S1-nuclease-PFGE hybridization PCR based replicon A ∼97 kb...
There is urgent need to develop novel treatment strategies reduce antimicrobial resistance. Collateral sensitivity (CS), where resistance one increases susceptibility other drugs, might enable selection against during treatment. However, the success of this approach would depend on conservation CS networks across genetically diverse bacterial strains. Here, we examine Escherichia coli strains isolated from urinary tract infections. We determine collateral susceptibilities mutants resistant...
The recent introduction of novel β-lactam/β-lactamase inhibitor combinations like ceftazidime-avibactam has increased our ability to treat infections caused by multidrug-resistant Gram-negative bacteria, including carbapenemase-producing Enterobacterales . However, the increasing number cases reported resistance is a concern. OXA-48 carbapenemase that no significant effect on ceftazidime, but inhibited avibactam. Since isolates with frequently harbor extended-spectrum β-lactamases are...
The clonal diversity underpinning trends in multidrug resistant Escherichia coli causing bloodstream infections remains uncertain. We aimed to determine the contribution of individual clones resistance over time, using large-scale genomics-based molecular epidemiology.This was a longitudinal, E population, genomic, cohort study that sampled isolates from 22 512 included Norwegian surveillance programme on microbes (NORM) 2002 2017. 15 laboratories were able share their isolates, and first...
Antibiotic resistant
Background The war in Ukraine led to migration of Ukrainian people. Early 2022, several European national surveillance systems detected multidrug-resistant (MDR) bacteria related patients. Aim To investigate the genomic epidemiology New Delhi metallo-β-lactamase (NDM)-producing Providencia stuartii from patients among countries. Methods Whole-genome sequencing 66 isolates sampled 2022–2023 10 countries enabled whole-genome multilocus sequence typing (wgMLST), identification resistance genes,...
Most antimicrobial peptides have an amphipathic, cationic structure, and effect on the cytoplasmic membrane of susceptible bacteria has been postulated as main mode action. Other mechanisms reported, including inhibition cellular functions by binding to DNA, RNA proteins, DNA and/or protein synthesis. Lactoferricin B (Lfcin B), a peptide derived from bovine lactoferrin, exerts slow inhibitory bactericidal activity does not lyse bacteria, indicating possible intracellular target. In present...
Whole-genome sequence analyses revealed the presence of blaNDM-1 (n = 31), blaGES-5 8), blaOXA-232 1), or blaNDM-5 1) in extensively drug-resistant and pandrug-resistant Enterobacteriaceae organisms isolated from in-patients 10 private hospitals (2012 to 2013) Durban, South Africa. Two novel NDM-1-encoding plasmids Klebsiella pneumoniae were circularized by PacBio sequencing. In p19-10_01 [IncFIB(K); 223.434 bp], was part a Tn1548-like structure (16.276 bp) delineated IS26 The multireplicon...
The prevalence of carbapenemase-producing Enterobacteriaceae (CPE) is increasing worldwide. Here we present associated patient data and molecular, epidemiological phenotypic characteristics all CPE isolates in Norway from 2007 to 2014 confirmed at the Norwegian National Advisory Unit on Detection Antimicrobial Resistance. All were characterized pheno- genotypically, including by whole genome sequencing (WGS). Patient reviewed retrospectively. In total 59 identified 53 patients. Urine was...
Cefiderocol is a novel siderophore β-lactam with improved hydrolytic stability toward β-lactamases, including carbapenemases, achieved by combining structural moieties of two clinically efficient cephalosporins, ceftazidime and cefepime. Consequently, cefiderocol represents treatment alternative for infections caused MDR Gram-negatives.To study the role on resistance development evolution β-lactamases from all Ambler classes, KPC-2, CTX-M-15, NDM-1, CMY-2 OXA-48.Directed evolution, using...
Abstract Opportunistic bacterial pathogen species and their strains that colonise the human gut are generally understood to compete against both each other commensal colonising this ecosystem. Currently we lacking a population-wide quantification of strain-level colonisation dynamics relationship potential prevalence in disease, how ecological factors might be modulating these. Here, using combination latest high-resolution metagenomics genomic epidemiology methods performed characterisation...
Whole genome sequencing data of 874 Escherichia coli isolates carrying blaNDM-5 from 13 European Union/European Economic Area countries between 2012 and June 2022 showed the predominance sequence types ST167, ST405, ST410, ST361 ST648, an increasing frequency detection. Nearly a third (30.6%) these were associated with infections more than half (58.2%) predicted to be multidrug-resistant. Further spread E. would leave limited treatment options for serious infections.
Abstract Escherichia coli is a leading cause of invasive bacterial infections in humans. Capsule polysaccharide has an important role pathogenesis, and the K1 capsule been firmly established as one most potent types E. through its association with severe infections. However, little known about distribution, evolution functions across phylogeny, which fundamental to elucidating expansion successful lineages. Using systematic surveys isolates, we show that K1- cps locus present quarter...