A5020661850- Escherichia coli research studies
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Mycobacterium research and diagnosis
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Viral gastroenteritis research and epidemiology
- Tuberculosis Research and Epidemiology
- Veterinary medicine and infectious diseases
- Gut microbiota and health
- Helicobacter pylori-related gastroenterology studies
- Cystic Fibrosis Research Advances
- Pneumonia and Respiratory Infections
- Immune responses and vaccinations
- Health and Lifestyle Studies
- Probiotics and Fermented Foods
- Bacterial Identification and Susceptibility Testing
- Medieval and Classical Philosophy
- Scientific Research and Technology
- RNA and protein synthesis mechanisms
- Lung Cancer Research Studies
- Cancer Research and Treatments
- Mobile Health and mHealth Applications
Benemérita Universidad Autónoma de Puebla
2023-2025
Centro Medico Nacional Siglo XXI
2015-2024
Mexican Social Security Institute
2014-2024
Hospital de Especialidades
2019
University of Florida
2013-2016
Universidad Nacional Autónoma de México
2007-2014
Aix-Marseille Université
2013
Centre National de la Recherche Scientifique
2013
Inserm
2013
Hospital Virgen de la Salud
2000
Klebsiella pneumoniae is an opportunistic pathogen frequently associated with nosocomially acquired infections. Host cell adherence and biofilm formation of K. isolates mediated by type 1 (T1P) 3 (MR/K) pili whose major fimbrial subunits are encoded the fimA mrkA genes, respectively. The E. coli common pilus (ECP) adhesive structure produced all pathogroups a homolog ecpABCDE operon present in genome. In this study, we aimed to determine prevalence these three genes among collection 69...
Abstract Here, we conducted a comprehensive analysis of 356 Klebsiella pneumoniae species complex (KpSC) isolates that were classified as classical (cl), presumptive hypervirulent (p-hv) and hypermucoviscous-like (hmv-like). Overall, K. (82.3%), variicola (2.5%) quasipneumoniae identified. These comprised 321 cl-KpSC, 7 p-hv-KpSC 18 hmv-like-KpSC. A large proportion cl-KpSC extended-spectrum-β-lactamases (ESBLs)-producers (64.4%) 3.4% colistin-resistant carrying carbapenemase ESBL genes. All...
Toxin-antitoxin (TA) modules are widely prevalent in both bacteria and archaea. Originally described as stabilizing elements of plasmids, TA also widespread on bacterial chromosomes. These promote persistence response to specific environmental stresses. So far, the possibility that could be involved virulence has been largely neglected, but recent comparative genomic studies have shown presence is significantly associated with pathogenicity bacteria. Using Salmonella a model, we investigated...
Klebsiella pneumoniae is an opportunistic pathogen causing nosocomial infections. Main virulence determinants of K. are pili, capsular polysaccharide, lipopolysaccharide and siderophores. The histone-like nucleoid-structuring protein (H-NS) a pleiotropic regulator found in several gram-negative pathogens. It has functions both as architectural component the nucleoid global gene expression. We generated Δhns mutant evaluated role H-NS on features pneumoniae. A down-regulated mrkA pilin...
Abstract Escherichia coli is a leading cause of invasive bacterial infections in humans. Capsule polysaccharide has an important role pathogenesis, and the K1 capsule been firmly established as one most potent types E. through its association with severe infections. However, little known about distribution, evolution functions across phylogeny, which fundamental to elucidating expansion successful lineages. Using systematic surveys isolates, we show that K1- cps locus present quarter...
Summary The ompS1 gene encodes a quiescent porin in Salmonella enterica. We analysed the effects of H‐NS and StpA, paralogue H‐NS, on expression. In an hns single mutant expression was derepressed but did not reach maximum level. Expression stpA showed same low repressed level as wild type. contrast, background, OmpS1 became abundant outer membrane. positively regulated by LeuO, LysR‐type regulator that has been involved pathogenesis. Upon induction cloned leuO into type, completely detected...
Klebsiella oxytoca toxigenic strains represent a critical health threat, mainly due to their link antibiotic-associated hemorrhagic colitis. This serious condition results from the bacteria’s ability produce tilimycin and tilivalline cytotoxins. Our research highlights pivotal role of OmpR, key regulator within EnvZ/OmpR two-component system, in controlling virulence factors associated with K. oxytoca. findings strongly indicate that OmpR is repressor aroX npsA genes, first genes NRPS...
Salmonella enterica can cause intestinal or systemic infections in humans and animals mainly by the presence of pathogenicity islands SPI-1 SPI-2, containing 39 44 genes, respectively. The AraC-like regulator HilD positively controls expression as well many other virulence genes including those located SPI-2. A previous report indicates that two-component system CpxR/A regulates genes: absence sensor kinase CpxA, but not its cognate response CpxR, reduces their expression. cell envelope...
Uropathogenic Escherichia coli (UPEC) strains cause urinary tract infections and employ type 1 P pili in colonization of the bladder kidney, respectively. Most intestinal extra-intestinal E. produce a pilus called common (ECP) involved cell adherence biofilm formation. However, contribution ECP to interaction UPEC with uroepithelial cells remains be elucidated. Here, we report that prototypic CFT073 F11 mutated major pilin structural gene ecpA are significantly deficient cultured HeLa...
Avian pathogenic Escherichia coli (APEC) strains cause systemic and localized infections in poultry, jointly termed colibacillosis. colibacillosis is responsible for significant economic losses to the poultry industry due disease treatment, decrease growth rate egg production, mortality. APEC are also considered a potential zoonotic risk humans. Fully elucidating virulence of key designing successful strategies against their transmission. Herein, we investigated prevalence newly discovered...
Helicobacter pylori is a Gram-negative bacterium that colonizes the human gastric mucosa and causes peptic ulcers carcinoma. H. strain 26695 has small genome (1.67 Mb), which codes for few known transcriptional regulators control bacterial metabolism virulence. We analyzed by qRT-PCR expression of 16 in 26695, including three sigma factors under different environmental conditions. When bacteria were exposed to acidic pH, urea, nickel or iron, differentially expressed with particularly strong...
Enterobacter cloacae has emerged as an opportunistic pathogen in healthcare-associated infections. Analysis of the genomic sequences several E. strains revealed presence genes that code for expression at least one type VI secretion system (T6SS). Here, we report strain ATCC 13047 codes two functional T6SS named T6SS-1 and T6SS-2. T6SS-2 were preferentially expressed tryptic soy broth tissue culture medium (DMEM), respectively. Mutants T6SS-1-associated clpV1 hcp1 significantly affected their...
ABSTRACT Atypical enteropathogenic Escherichia coli (aEPEC) strains are diarrheal pathogens that lack bundle-forming pilus production but possess the virulence-associated locus of enterocyte effacement. aEPEC strain 1551-2 produces localized adherence (LA) on HeLa cells; however, its isogenic intimin ( eae ) mutant a diffuse-adherence (DA) pattern. In this study, we aimed to identify DA-associated adhesin mutant. Electron microscopy identified rigid rod-like pili composed an 18-kDa protein,...
Enterohemorrhagic Escherichia coli (EHEC) is a significant cause of serious human gastrointestinal disease worldwide. EHEC strains contain pathogenicity island called the locus enterocyte effacement (LEE), which encodes virulence factors responsible for damaging gut mucosa. The Cpx envelope stress response E. controlled by two-component system (TCS) consisting sensor histidine kinase (CpxA) and cytoplasmic regulator (CpxR). In this study, we investigated role CpxRA in expression LEE-encoded...
Hypermucoviscosity (hmv) is a capsule-associated phenotype usually linked with hypervirulent Klebsiella pneumoniae strains. The key components of this are the RmpADC proteins contained in non-transmissible plasmids identified and studied K. . variicola closely related to recently has been as an emergent human pathogen. variicol normally contains plasmids, some them carrying antibiotic resistance virulence genes. Previously, we described clinical isolate showing hmv-like that harbors 343-kb...
The genome of Mycobacterium tuberculosis (Mtb) harbors the genetic machinery for assembly Fimbrial low-molecular-weight protein (Flp) type IV pilus. Presumably, Flp pilus is essential pathogenesis. However, it remains unclear whether pili genes are transcribed in culture or during infection host cells. This study aimed to shed light on expression pili-assembly (tadZ, tadA, tadB, tadC, flp, tadE, and tadF) Mtb growing under different growth conditions (exponential phase, stationary dormancy...
Klebsiella pneumoniae is recognized as a common cause of nosocomial infections and outbreaks causing pneumonia, septicemia, urinary tract infections. This opportunistic bacterium shows an increasing acquisition antibiotic-resistance genes, which complicates treatment Hence, fast reliable strain typing methods are paramount for the study this pathogen’s multi-drug resistance genetic profiles. In study, thirty-eight strains K. isolated from blood pediatric patients were characterized by...
Helicobacter pylori is a Gram-negative bacterium that colonizes the human gastric mucosa and responsible for causing peptic ulcers carcinoma. The expression of virulence factors allows persistence H. in stomach, which results chronic, sometimes uncontrolled inflammatory response. Type II toxin-antitoxin systems have emerged as important many pathogenic bacteria. Three type (TA) previously been identified genome 26695: HP0315-HP0316, HP0892-HP0893, HP0894-HP0895. Here we characterized...
Avian pathogenic Escherichia coli (APEC) causes localized and systemic avian infections is responsible for considerable economic losses in the poultry industry. This organism adheres invades human cells, however, regulatory networks that dictate its virulence are largely unknown. The CpxRA two-component system sensing controlling outer-membrane stress detecting misfolded proteins bacterial periplasmic space. CpxA a membrane sensor kinase CpxR cytoplasmic transcriptional regulator. In this...
Klebsiella pneumoniae successfully colonizes host tissues by recognizing and interacting with cholesterol present on membrane-associated lipid rafts. In this study, we evaluated the role of in expression capsule polysaccharide genes K. its implication resistance to phagocytosis. Our data revealed that exogenous added increases macrophage-mediated To explain event, capsular galF, wzi manC was determined presence cholesterol. Down-regulation these occurred leading increased susceptibility...
ABSTRACT The toxigenic Klebsiella oxytoca strains secret the tilivalline enterotoxin, which causes antibiotic-associated hemorrhagic colitis. is a non-ribosomal peptide synthesized by enzymes encoded in two divergent operons clustered pathogenicity island. transcriptional regulator Lrp ( l eucine-responsive r egulatory p rotein) controls expression of several bacterial genes involved virulence. In this work, we determined aroX and npsA , first each biosynthetic operon K. MIT 09-7231...
Mycobacterium tuberculosis and M. smegmatis form drug-tolerant biofilms through dedicated genetic programs. In support of a stepwise process regulating biofilm production in mycobacteria, it was shown elsewhere that lsr2 participates intercellular aggregation, while groEL1 required for maturation smegmatis. Here, by means RNA-Seq, we monitored the early steps bovis BCG, to distinguish aggregation from attachment surface. Genes encoding transcriptional regulators dosR BCG0114 (Rv0081) were...
Abstract A single intradermal vaccination with an antibiotic-less version of BCGΔBCG1419c given to guinea pigs conferred a significant improvement in outcome following low dose aerosol exposure M. tuberculosis compared that provided by BCG Pasteur. was more attenuated than murine macrophages, athymic, BALB/c, and C57BL/6 mice. In pigs, at least as induced similar dermal reactivity BCG. Vaccination resulted increased anti-PPD IgG those receiving Guinea vaccinated showed reduction replication...
<b><i>Background:</i></b> Gram-negative bacilli are the most common bacteria causing nosocomial bloodstream infections (NBSIs) in Latin American countries. <b><i>Methods:</i></b> The antibiotic resistance profiles of isolated from blood cultures pediatric patients with NBSIs over a 3-year period tertiary care hospital Mexico City were determined using VITEK-2 system. Sixteen antibiotics tested to ascertain rate and minimum inhibitory...