A5011681584- Antibiotic Resistance in Bacteria
- Enterobacteriaceae and Cronobacter Research
- Bacterial Identification and Susceptibility Testing
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Antibiotics Pharmacokinetics and Efficacy
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotic Use and Resistance
- Antimicrobial Resistance in Staphylococcus
- Escherichia coli research studies
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Urinary Tract Infections Management
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
- Nosocomial Infections in ICU
- Salmonella and Campylobacter epidemiology
- Infections and bacterial resistance
- Essential Oils and Antimicrobial Activity
- Biochemical and Structural Characterization
- Lipid Membrane Structure and Behavior
- Antimicrobial Peptides and Activities
- Pneumonia and Respiratory Infections
- Microbial Metabolites in Food Biotechnology
- Plant Pathogenic Bacteria Studies
Instituto Nacional de Salud Pública
2015-2024
Hospital del Niño
2014
Centro Médico Nacional La Raza
2012
Mexican Social Security Institute
2012
CNR de la Résistance aux Antibiotiques
2011
To characterize the microbiological, molecular and epidemiological data of an outbreak carbapenem-resistant Enterobacteriaceae (CRE) in a tertiary-care hospital Mexico.From September 2014 to July 2015, all CRE clinical isolates recovered during Hospital Civil "Fray Antonio Alcalde" Jalisco, Mexico were screened for antimicrobial susceptibility, carbapenemase production, carbapenemase-encoding genes, plasmid profiles. Horizontal transfer imipenem resistance; clonal diversity by pulsed-field...
The pUM505 plasmid, isolated from a clinical Pseudomonas aeruginosa isolate, confers resistance to ciprofloxacin (CIP) when transferred into the standard P. strain PAO1. CIP is an antibiotic of quinolone family that used treat infections. In silico analysis, performed identify genes, revealed 65-amino-acid product encoded by orf131 gene in displays 40% amino acid identity Mycobacterium smegmatis aminoglycoside phosphotransferase (an enzyme phosphorylates and inactivates antibiotics). We...
Acinetobacter baumannii is an emergent bacterial pathogen that provokes many types of infections in hospitals around the world. The genome this organism consists a chromosome and plasmids. These plasmids vary over wide size range them have been linked to acquisition antibiotic-resistance genes. Our bioinformatic analyses indicate A. belong small number plasmid lineages. general structure these lineages seems be very stable not only genes involved maintenance functions but gene sets encoding...
Abstract Here, we conducted a comprehensive analysis of 356 Klebsiella pneumoniae species complex (KpSC) isolates that were classified as classical (cl), presumptive hypervirulent (p-hv) and hypermucoviscous-like (hmv-like). Overall, K. (82.3%), variicola (2.5%) quasipneumoniae identified. These comprised 321 cl-KpSC, 7 p-hv-KpSC 18 hmv-like-KpSC. A large proportion cl-KpSC extended-spectrum-β-lactamases (ESBLs)-producers (64.4%) 3.4% colistin-resistant carrying carbapenemase ESBL genes. All...
Klebsiella variicola was very recently described as a new bacterial species and is closely related to pneumoniae; in fact, K. isolates were first identified pneumoniae. Therefore, it might be the case that some isolates, which initially classified pneumoniae, are actually variicola. The aim of this study devise multiplex-PCR probe can differentiate from these sister species.This work describes development method identify This based on sequencing clinical isolate (801) comparing other...
We aimed to assess the resistance rates of antimicrobial-resistant, in bacterial pathogens epidemiological importance 47 Mexican centers.In this retrospective study, we included a stratified sample centers, covering 20 states. Selected isolates considered as potential causatives disease collected over 6-month period were included. Laboratories employed their usual methods perform microbiological studies. The results deposited into database and analyzed with WHONET 5.6 software.In total...
Bacteria that produce the broad-spectrum Carbapenem antibiotic New Delhi Metallo-β-lactamase (NDM) place a burden on health care systems worldwide, due to limited treatment options for infections caused by them and rapid global spread of this resistance mechanism. Although it is believed associated gene blaNDM-1 originated in Acinetobacter spp., role Enterobacteriaceae its dissemination remains unclear. In study, we used whole genome sequencing investigate dynamics blaNDM-1-positive plasmids...
Objective. Due to the fact that K. variicola, quasipneumoniae and pneumoniae are closely related bacterial species, misclassification can occur due mistakes either in normal biochemical tests or during submission public databases. The objective of this work was identify variicola quasipneumoniae genomes misclassified GenBank database. Materials methods. Both rpoB phylogenies average nucleotide identity (ANI) were used a significant number Klebsiella spp. genomes. Results. Here we report an...
This report presents phenotypic and genetic data on the prevalence characteristics of extended-spectrum β-lactamases (ESBLs) representative carbapenemases-producing Gram-negative species in Mexico.A total 52 centers participated, 43 hospital-based laboratories 9 external laboratories. The distribution antimicrobial resistance for Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae complex, Acinetobacter baumannii Pseudomonas aeruginosa selected clinical specimens from January 1 to...
ABSTRACT Between 1997 and 2000 a single multidrug-susceptible methicillin-resistant Staphylococcus aureus clone, M (sequence type 30 [ST30]-staphylococcal cassette chromosome mec [SCC ] IV), was present in pediatric hospital Mexico City, Mexico. In 2001 the international multidrug-resistant New York-Japan clone (ST5-SCC II) introduced into hospital, completely replacing by 2002.
During 2003, 40 carbapenem-resistant Pseudomonas aeruginosa clinical isolates collected in a Mexican tertiary-care hospital were screened for metallo-beta-lactamase production. Thirteen produced IMP-15, and 12 had single pulsed-field gel electrophoresis pattern. The bla(IMP-15) gene cassette was inserted plasmid-borne integron with unique array of cassettes named In95.
ABSTRACT A novel QnrB-like plasmid-mediated resistance determinant, QnrB19, was identified from an Escherichia coli clinical isolate Colombia. Its gene associated with IS Ecp1 -like insertion element that did not act as a promoter for its expression. Using in vitro model of transposition, we showed the able to mobilize qnrB19 gene.
Introduction Patients with hematologic malignancies have greater risk-factors for primary bloodstream infections (BSI). Methods From 2004–2009, we analyzed bacteremia caused by extended-spectrum beta-lactamase Escherichia coli (ESBL-EC) (n = 100) and compared cephalosporin-susceptible E. in patients malignancies. Objective To assess the clinical features, risk factors, outcome of ESBL-EC BSI malignancies, to study molecular epidemiology isolates. Results The main diagnosis was acute leukemia...
This work describes the characterization of plasmid-mediated quinolone-resistance (PMQR) genes from a multicenter study ESBL-producing Enterobacteriaceae pediatric clinical isolates in Mexico. The PMQR gene-positive were characterized with respect to ESBLs, and mutations GyrA ParC proteins determined. phylogenetic relationship was established by PFGE transfer determined mating assays. prevalence 32.1%, rate qnr-positive 15.1%; 93.3% latter qnrB 6.4% qnrA1. distribution terms bacterial...
Cronobacter spp. are bacterial pathogens that cause neonatal meningitis, septicemia, and necrotizing enterocolitis in infants with a lethality rate of 40-80%. Powdered infant formulas (PIF) have been implicated as the main vehicles transmission. The organism can also infection through contaminated expressed breast milk, has recovered from feeding tubes neonates not fed reconstituted PIF. This study analysed sakazakii Enterobacter PIF, fecal matter, milk kitchen environment an diarrheic...
In this study, we report the carbapenemase-encoding genes and colistin resistance in Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa second year of COVID-19 pandemic. Clinical isolates included carbapenem-resistant K. E. A. P. aeruginosa. Carbapenemase-encoding were detected by PCR. Carbapenem-resistant pneumoniae coli analyzed using Rapid Polymyxin NP assay. mcr screened Pulsed-field gel electrophoresis whole-genome sequencing performed on...
ObjectivesTo study the prevalence, risk factors, outcome, and molecular epidemiology in patients with bacteremia caused by extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae (Kp) (cases), comparison a susceptible Kp (controls).MethodsThis was retrospective case–control including all episodes of for period 1993 to 2002 at referral hospital adults Mexico. ESBL production tested E-test. All isolates were typed pulsed field gel electrophoresis (PFGE). A subset underwent plasmid...
The objectives of this study were to investigate the prevalence plasmid-mediated quinolone resistance genes in a collection 226 extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae isolates and characterize qnr-positive isolates. rate was 21.6% (49/226), 49.5% for aac(6')-Ib-cr (112/226), 1.7% qepA1 (4/226). Those carried qnr corresponding types qnrB (71.4%), qnrS1 (24.4%), qnrA1 (18.3%). distribution among bacterial species as follows: 55.8% (19/34) Enterobacter cloacae, 50%...