Cervical cancer (CC) is caused by high-risk human papillomavirus persistence due to the immunosuppressive tumor microenvironment mediated cytokines. Vaginal microbiota determines presence of certain cytokines locally. We assessed association between cervical diversity and histopathological diagnosis each stage CC, we evaluated mRNA expression levels IL-4, IL-6, IL-10, TGF-β1, TNF-α IFN-γ across specific bacterial clusters. determined high throughput sequencing 16S rDNA amplicons classified...

Klebsiella variicola was very recently described as a new bacterial species and is closely related to pneumoniae; in fact, K. isolates were first identified pneumoniae. Therefore, it might be the case that some isolates, which initially classified pneumoniae, are actually variicola. The aim of this study devise multiplex-PCR probe can differentiate from these sister species.This work describes development method identify This based on sequencing clinical isolate (801) comparing other...

Background: The study of human B cell response to dengue virus (DENV) infection is critical understand serotype-specific protection and the cross-reactive sub-neutralizing response.Whereas first beneficial thus represents ultimate goal vaccination, latter has been implicated in development severe disease, which occurs a small, albeit significant, fraction secondary DENV infections.Both primary infections are associated with production poly-reactive IgG antibodies.Methods: To gain insight...

The origin and function of blood IgM+IgD+CD27+ B cells is controversial, they are considered a heterogeneous population. Previous staining circulating healthy donors with rotavirus fluorescent virus-like particles allowed us to differentiate two subsets IgM+IgD+CD27+: IgMhi IgMlo cells. Here, we confirmed this finding compared the phenotype, transcriptome, in vitro function, Ig gene repertoire these subsets. Eleven markers phenotypically discriminated both (CD1c, CD69, IL21R, CD27, MTG,...

Abstract Background Human Malaria is transmitted by mosquitoes of the genus Anopheles . Transmission a complex phenomenon involving biological and environmental factors humans, parasites mosquitoes. Among more than 500 anopheline species, only few species from different branches mosquito evolutionary tree transmit malaria, suggesting that their vectorial capacity has evolved independently. albimanus (subgenus Nyssorhynchus ) an important malaria vector in Americas. The divergence time...

Background: Despite the potential to produce antibodies that can neutralize different virus (heterotypic neutralization), there is no knowledge of why vaccination against influenza induces protection predominantly utilized viral strains (homotypic response).Identification structural patterns B cell repertoire associated heterotypic neutralization may contribute identify relevant epitopes for a universal vaccine influenza.Methods: Blood samples were collected from volunteers immunized with...

The symbiotic plasmid of Rhizobium etli CE3 belongs to the RepABC family replicons. This is characterized by presence three conserved genes, repA, repB, and repC, encoded same DNA strand. A long intergenic sequence (igs) between repB repC also in all members family. In this paper we demonstrate that (i) repABC genes are organized an operon; (ii) RepC product essential for replication; (iii) RepA RepB products participate segregation regulation copy number; (iv) there two cis-acting...

Three endophytic Klebsiella variicola isolates-T29A, 3, and 6A2, obtained from sugar cane stem, maize shoots, banana leaves, respectively-were used for whole-genome sequencing. Here, we report the draft genome sequences of circular chromosomes plasmids. The genomes contain plant colonization cellulases genes. This study will help toward understanding genomic basis K. interaction with hosts.

The B cell antigen receptor repertoire is highly diverse and constantly modified by clonal selection. High-throughput DNA sequencing (HTS) of the lymphocyte (Rep-Seq) represents a promising technology to explore such diversity ex-vivo assist in identification antigen-specific antibodies based on molecular signatures Therefore, integrative tools for reconstruction analysis from antibody sequences are needed. We developed ImmunediveRity, stand-alone pipeline primarily R programming integral...

ABSTRACT Adenylate cyclases (ACs) catalyze the formation of 3′,5′-cyclic AMP (cAMP) from ATP. A novel AC-encoding gene, cyaC , was isolated Rhizobium etli by phenotypic complementation an Escherichia coli cya mutant. The functionality gene corroborated its ability to restore cAMP accumulation in E. Further, overexpression a malE :: fusion protein allowed detection significant AC activity levels cell extracts CyaC is unrelated any known or other exhibiting currently function. Thus, represents...

The basic replicon of Rhizobium etli CE3, like other members the repABC plasmid family, is constituted by operon. RepC essential for replication, and RepA RepB play a role in segregation. It has been shown that deletion derivatives lacking repAB genes have an increased copy number, indicating these participate control number. also trans ‐incompatibility factor. To understand regulation operon, this paper: (i) transcription start site operon was determined; (ii) promoter region identified...

ABSTRACT Enteropathogenic Escherichia coli uses a type III secretion system (T3SS), encoded in the locus of enterocyte effacement (LEE) pathogenicity island, to translocate wide repertoire effector proteins into host cell order subvert signaling cascades and promote bacterial colonization survival. Genes encoding III-secreted effectors are located LEE scattered throughout chromosome. While gene regulation is better understood, conditions factors involved expression outside just starting be...

Influenza viruses display a high mutation rate and complex evolutionary patterns. Next-generation sequencing (NGS) has been widely used for qualitative semi-quantitative assessment of genetic diversity in biological samples. The "deep sequencing" approach, enabled by the enormous throughput current NGS platforms, allows identification rare viral variants targeted regions, but is usually limited to small number samples.We designed proof-of-principle study test whether redistributing from...

Chagas disease is a parasitic infection caused by Trypanosoma cruzi. It an important public health problem affecting around seven to eight million people in the Americas. A large number of hematophagous triatomine insect species, occupying diverse natural and human-modified ecological niches transmit this disease. Triatomines are long-living hemipterans that have evolved explode different habitats associate with their vertebrate hosts. Understanding molecular basis extreme physiological...

Innate lymphoid cells (ILC) are members of a heterogeneous family with origin that mimics the T helpers (Th) cytokine profile. ILC involved in early effector cytokine-mediated responses during infections peripheral tissues. also play an important role chronic skin inflammatory diseases, including psoriasis. Although classical express CD127, it has been recently reported presence non-classical CD127- populations and CD127low precursor (EILP). development predominately investigated mouse...

Antibiotic resistance has emerged as a global threat to public health, generating growing interest in investigating the presence of antibiotic-resistant bacteria environments influenced by anthropogenic activities. Wastewater treatment plants hospital serve significant reservoirs antimicrobial-resistant bacteria, where favorable environment is established, promoting proliferation and transfer genes among different bacterial species. In our study, we isolated total 243 strains from 5...

Carbapenem-resistant Gram-negative bacteria isolated in Venezuela have been poorly characterized. The present study characterized a total of 34 isolates obtained from 27 patients; five these patients were multi-infected. bacterial species identified Klebsiella pneumoniae (17), Pseudomonas aeruginosa (9), and Acinetobacter baumannii (8). From isolates, 85% as carbapenemase-producing bacteria, the carbapenemase genes blaKPC-2 (10/29 [34.4%]), blaVIM-type (7/29 [24.1%]), blaOXA-23 blaNDM-1...

For malaria transmission, Plasmodium parasites must develop in the mosquito vector. Oxidative stress insect midgut, triggered by environmental changes (e.g., pH and temperature), influences cellular signaling involved differentiation from gametocytes to mobile ookinetes for purpose of parasite survival. activates homeostatic response characterized phosphorylation eIF2α, attenuation protein synthesis, transcription genes participating unfolded antioxidant processes, forming a part an...

The use of colistin in food-producing animals favors the emergence and spread colistin-resistant strains. Here, we investigated occurrence molecular mechanisms resistance among E. coli isolates from a Mexican piglet farm. A collection 175 cephalosporin-resistant colonies swine fecal samples were recovered. phenotype was identified by rapid polymyxin test mcr-type genes screened PCR. We assessed strains antimicrobial susceptibility test, pulse-field gel electrophoresis, plasmid profile,...

High-throughput sequencing of the antibody repertoire is enabling a thorough analysis B cell diversity and clonal selection, which may improve novel discovery process. Theoretically, an adequate bioinformatic could allow identification candidate antigen-specific antibodies, requiring their recombinant production for experimental validation specificity. Gene synthesis commonly used generation antibodies identified in silico. Novel strategies that bypass gene offer more accessible...

Listeriolysin O (LLO) has been proposed as a potential carrier or adjuvant molecule in the vaccination field. However, cytotoxic and pro-apoptotic effects of LLO are major limitations for this purpose. Here, we have performed preclinical safety evaluation characterized new application non-cytolytic mutant (dtLLO) to enhance modulate immune response against envelope (E) protein from dengue virus. In addition, studied dtLLO on human cells role membrane cholesterol binding proinflammatory...

Background The axolotl, Ambystoma mexicanum is a unique biological model for complete tissue regeneration. Is neotenic endangered species and highly susceptible to environmental stress, including infectious disease. In contrast other amphibians, the axolotl particularly vulnerable certain viral infections. Like salamanders, genome one of largest (32 Gb) impact size on Ig loci architecture unknown. To better understand immune response in we aimed characterize immunoglobulin A. compare it with...