A5047510395- Influenza Virus Research Studies
- Diabetes and associated disorders
- Insect Resistance and Genetics
- Mosquito-borne diseases and control
- Invertebrate Immune Response Mechanisms
- Immunotherapy and Immune Responses
- Malaria Research and Control
- SARS-CoV-2 detection and testing
- Insect symbiosis and bacterial influences
- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- Vector-borne infectious diseases
- Synthesis and Biological Activity
- Immunodeficiency and Autoimmune Disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Biosensors and Analytical Detection
- Microbial infections and disease research
- Transgenic Plants and Applications
- Dengue and Mosquito Control Research
- Bioactive Compounds and Antitumor Agents
- Immune responses and vaccinations
- Traditional and Medicinal Uses of Annonaceae
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Respiratory viral infections research
Instituto Nacional de Salud Pública
2007-2023
The EtOAc extract of the stem bark Hintonia latiflora showed suppression total parasitemia and chemosuppression schizont numbers, when tested in vivo against Plasmodium berghei infection mice. Bioassay-directed fractionation extract, using vitro 16 h 4-day tests on P. led to isolation new compound 5-O-β-d-glucopyranosyl-7,4'-dimethoxy-3'-hydroxy-4-phenylcoumarin (1), along with known 5-O-β-d-glucopyranosyl-7-methoxy-3',4'-dihydroxy-4-phenylcoumarin (2). structure 1 was established basis...
Abstract Background Human Malaria is transmitted by mosquitoes of the genus Anopheles . Transmission a complex phenomenon involving biological and environmental factors humans, parasites mosquitoes. Among more than 500 anopheline species, only few species from different branches mosquito evolutionary tree transmit malaria, suggesting that their vectorial capacity has evolved independently. albimanus (subgenus Nyssorhynchus ) an important malaria vector in Americas. The divergence time...
Background: Despite the potential to produce antibodies that can neutralize different virus (heterotypic neutralization), there is no knowledge of why vaccination against influenza induces protection predominantly utilized viral strains (homotypic response).Identification structural patterns B cell repertoire associated heterotypic neutralization may contribute identify relevant epitopes for a universal vaccine influenza.Methods: Blood samples were collected from volunteers immunized with...
Abstract Salivary glands of female mosquitoes produce proteins, not completely described yet, that participate in carbohydrate and blood feeding. Here, we report an acidic glycoprotein 35 kDa (GP35 ANOAL) secreted the saliva malaria vector mosquito Anopheles albimanus . GP35 ANOAL is produced exclusively distal lateral lobes adult salivary glands, it has a pI 4.45 negatively stained by regular silver stain. An 888 bp cDNA clone encoding predicted product 240 amino acids signal peptide,...
High-throughput sequencing of the antibody repertoire is enabling a thorough analysis B cell diversity and clonal selection, which may improve novel discovery process. Theoretically, an adequate bioinformatic could allow identification candidate antigen-specific antibodies, requiring their recombinant production for experimental validation specificity. Gene synthesis commonly used generation antibodies identified in silico. Novel strategies that bypass gene offer more accessible...
We aimed to estimate the lead time and infection prevalence from SARS-CoV-2 wastewater (WW) monitoring compared with clinical surveillance data in Mexico generate evidence about feasibility of a large-scale WW system. selected 10 treatment plants (WWTP) 5 COVID-19 hospitals major urban conglomerates collected biweekly 24-h flow-adjusted composite samples during October–November 2020. concentrated by polyethylene glycol precipitation employed quantitative PCR (RT-qPCR) assays, targeting...
In the southern Pacific coast of Chiapas, Mexico (SM), two most abundant vector species, Nyssorhynchus albimanus and Anopheles pseudopunctipennis, were susceptible to different Plasmodium vivax Pvs25/28 haplotypes. To broaden our understanding existing P. in area, genes encoding proteins relevant for ookinete development 18S rRNA studied. infectivity (percentage infected mosquitoes oocyst numbers) was evaluated by simultaneously feeding blood samples from patients Ny. An. pseudopunctipennis...
Antibody class switch recombination (CSR) to IgG, IgA or IgE is a hallmark of adaptive immunity, allowing antibody function diversification beyond IgM. CSR involves deletion the IgM/IgD constant region genes placing new acceptor Constant gene, downstream VDJH exon. depends on non-coding (CSRnc) transcription donor I and IH exons, located 5' upstream each CH coding gene. Although our knowledge role CSRnc has advanced greatly, its extension importance in healthy diseased humans scarce. We...
To gain a better understanding of the Zika virus (ZIKV) vector transmission in Mexico, we determined competence local population Ae. aegypti (Acapulco, Guerrero) for strain ZIKV isolated from Mexican febrile patient.Eggs were hatched and larvae reared under controlled conditions. After five days post-emergence, female mosquitoes fed an infectious blood-meal containing ZIKV. Mosquitoes analyzed at 4, 7 14-day post-infection (dpi). Infection (gut), dissemination (wings, legs heads) potential...
Abstract Background Antibody class switch recombination (CSR) to IgG, IgA or IgE is a hallmark of adaptive immunity, allowing antibody function diversification beyond IgM. CSR involves deletion the IgM/IgD constant region genes placing new acceptor Constant (C H ) gene, downstream VDJ exon. depends on non-coding (CSRnc) transcription donor Iμ and I exons, located 5’ upstream each C coding gene. Although our knowledge role CSRnc has advanced greatly, its extension importance in healthy...