A5020020793- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Celiac Disease Research and Management
- Malaria Research and Control
- Microscopic Colitis
- Helicobacter pylori-related gastroenterology studies
- Virology and Viral Diseases
- Pancreatitis Pathology and Treatment
- HIV Research and Treatment
- Vector-borne infectious diseases
- Biomedical Research and Pathophysiology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Autoimmune and Inflammatory Disorders Research
- Inflammation biomarkers and pathways
- Gastrointestinal motility and disorders
- Immunodeficiency and Autoimmune Disorders
- Neonatal and Maternal Infections
- Vibrio bacteria research studies
- Liver Diseases and Immunity
- Listeria monocytogenes in Food Safety
- Liver Disease Diagnosis and Treatment
- Psoriasis: Treatment and Pathogenesis
- Abdominal Trauma and Injuries
- Blood groups and transfusion
Providence College
2019-2024
University of Rhode Island
2019-2024
Universidad Veracruzana
2014-2023
Abstract Background The cost‐effectiveness for screening celiac disease ( CD ) in patients with irritable bowel syndrome IBS ), specifically the diarrhea ‐D) subtype, is beneficial if prevalence >1%. However, recent studies have shown controversial results. In this large case–control study, our aim was to determine of and a panel related antibodies diagnosed . Materials methods Four hundred (Rome III 400 asymptomatic healthy controls were prospectively evaluated using antihuman tissue...
Abstract Background Dengue virus (DENV) nonstructural protein 1 (NS1) has multiple functions within infected cells, on the cell surface, and in secreted form, is highly immunogenic. Immunity from previous DENV infections known to exert both positive negative effects subsequent infections, but contribution of NS1-specific antibodies these incompletely understood. Methods We investigated their significance infection. analyzed plasma samples collected a prospective cohort study prior...
The aims of this study were to determine the involvement interleukin 17 (IL-17) and IL-17-producing cells in dengue pathogenesis. Blood samples from virus (DENV)-infected patients collected on different days after onset symptoms. Patients classified according 1997 World Health Organization guidelines. Our examined 152 blood fever (DF, n = 109) hemorrhagic (DHF, 43) 90 healthy controls (HC). High serum concentrations IL-17A IL-22 also associated with DHF (IL-17A [DHF vs. DF, p < 0.01; HC,...
There is growing interest in understanding antibody (Ab) function beyond neutralization. The non-structural protein 1 (NS1) of Zika virus (ZIKV) an attractive candidate for effective vaccine as Abs against NS1, unlike the envelope or premembrane, do not carry risk mediating antibody-dependent enhancement. Our aim was to evaluate whether ZIKV NS1 elicited following natural infection humans can mediate cellular cytotoxicity (ADCC). We evaluated isotype specificity ZIKV-specific immune sera and...
Prior exposure to dengue virus (DENV) has a profound impact on the outcome of infection, which varies according interval between infections. Antibodies secreted by B cells and cytokines T are thought contribute both protective immunity against DENV pathogenesis disease. We analyzed peripheral blood mononuclear (PBMC) collected from Thai children over 5-year prospective cohort study define dynamics DENV-specific memory cell responses symptomatic or subclinical To measure responses, PBMC were...
Previous studies have revealed the clinical relevance of pro-inflammatory cytokine production during dengue virus (DENV) infections. In this study, we evaluated interleukin-21 (IL-21), a key soluble mediator mainly produced by CD4+ T cells. The aim study was to investigate role IL-21 course primary and secondary DENV infections potential association serum levels with disease pathogenesis. Blood samples from DENV-infected patients were collected on different days after onset symptoms....
Biodegradables Chitosan-based Nanoparticles (CS NPs) have been extensively studied as delivery system for therapeutic molecules and efficient carriers or adjuvants in experimental vaccination. Physicochemical association between CS NPs antigens is a key step the biological function carrier devices. However, adjuvant property, it not well known if coupling with vaccine required to potentiate immune response. To address this issue, work, we evaluated potential effect of by simply mixing two...
Listeriolysin O (LLO) has been proposed as a potential carrier or adjuvant molecule in the vaccination field. However, cytotoxic and pro-apoptotic effects of LLO are major limitations for this purpose. Here, we have performed preclinical safety evaluation characterized new application non-cytolytic mutant (dtLLO) to enhance modulate immune response against envelope (E) protein from dengue virus. In addition, studied dtLLO on human cells role membrane cholesterol binding proinflammatory...
A common hallmark of dengue infections is the dysfunction vascular endothelium induced by different biological mechanisms. In this paper, we studied role recombinant NS1 proteins representing four serotypes, and their in promoting expression release endocan, which a highly specific biomarker endothelial cell activation. We evaluated mRNA levels endocan protein vitro following stimulation HUVEC HMEC-1 lines with proteins. increase 48 h post-activation both lines. Endocan were higher cells...
Although most patients with celiac disease (CD) have digestive manifestations, in some of them they may be extraintestinal (atypical) nature, such as chronic anemia, ataxia, and fertility disorders.To determine the prevalence CD-related antibodies Mexican women disorders.Case-control study who attended evaluation for disorders a specialized center. h-tTG-IgA, gliadin IgA II IgG were quantified; titers > 30 IU considered positive.One-hundred seventy-one cases 171 controls included; 137...
Memory T cells resulting from primary dengue virus (DENV) infection are hypothesized to influence the clinical outcome of subsequent DENV infection. However, few studies involving prospectively collected blood samples have found weak and inconsistent associations with variable temporal trends in DENV-specific memory cell responses between subjects. This study used both ex-vivo cultured ELISPOT assays further evaluate serotype-cross-reactive severity secondary Using assays, frequencies...
Antecedentes: Aunque los pacientes con enfermedad celiaca (EC) tienen en su mayoría manifestaciones digestivas, algunos pueden presentarlas de índole extraintestinal (atípicas), como anemia crónica, ataxia y trastornos la fertilidad.Objetivo: Determinar prevalencia anticuerpos relacionados EC mujeres mexicanas fertilidad.Material métodos: Estudio casos controles que acudieron a valoración por fertilidad un centro especializado.Se cuantificó h-tTG IgA, gliadina IgA II IgG II; títulos > 30 UI...
Celiac disease (CD) is a chronic immune-mediated enteropathy triggered by exposure to dietary gluten in genetically predisposed individuals. In contrast, irritable bowel syndrome (IBS) common functional gastrointestinal disorder affecting the large intestine, without an autoimmune component. Here, we evaluated prevalence of IgA and IgG antibodies maize zeins (AZA) patients with CD IBS. Using in-house ELISA assay, anti-zein serum 37 newly diagnosed (16 biopsy proved 21 serological diagnosis)...