A5007722047- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Enterobacteriaceae and Cronobacter Research
- Escherichia coli research studies
- Vibrio bacteria research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial biofilms and quorum sensing
- Antibiotic Use and Resistance
- Antibiotics Pharmacokinetics and Efficacy
- Mycobacterium research and diagnosis
- Pneumonia and Respiratory Infections
- Gut microbiota and health
- Salmonella and Campylobacter epidemiology
- Tuberculosis Research and Epidemiology
- Infections and bacterial resistance
- Antimicrobial Resistance in Staphylococcus
- European and International Law Studies
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Marine and fisheries research
- Urinary Tract Infections Management
- Streptococcal Infections and Treatments
- Listeria monocytogenes in Food Safety
- Auditing, Earnings Management, Governance
- Experimental Behavioral Economics Studies
Statens Serum Institut
2015-2024
Gentofte Hospital
2018
Norges Bank
2016
National Aquatic Resources Research and Development Agency
1998-2009
The plasmid-mediated colistin resistance gene, mcr-1 , was detected in an Escherichia coli isolate from a Danish patient with bloodstream infection and five E. isolates imported chicken meat. One meat belonged to the epidemic spreading sequence type ST131. In addition IncI2*, incX4 replicon found be linked . This report follows recent detection of animals, food humans China.
Extraintestinal pathogenic Escherichia coli (ExPEC) is the main cause of urinary tract infections and septicemia. Significant attention has been given to ExPEC sequence type ST131, which categorized as a “high-risk” clone. High-risk clones are globally distributed associated with various antimicrobial resistance determinants, ease transmission, persistence in hosts, effective transmission between hosts. The high-risk have enhanced pathogenicity severe and/or recurrent infections. We show...
ABSTRACT The aim of this study was to construct a valid publicly available method for in silico fimH subtyping Escherichia coli particularly suitable differentiation fine-resolution subgroups within clonal groups defined by standard multilocus sequence typing (MLST). FimTyper constructed as FASTA database containing all currently known alleles. software source code is at https://bitbucket.org/genomicepidemiology/fimtyper , the freely https://bitbucket.org/genomicepidemiology/fimtyper_db and...
ObjectivesTo compare and characterize extended-spectrum β-lactamase (ESBL)-producing Escherichia coli from pigsties, pig farmers their families on farms with previous high or no use of third- fourth-generation cephalosporins.
ABSTRACT The lack of novel antibiotics against gram-negative bacteria has reinstated polymyxins as the drugs last resort to treat serious infections caused by extremely multiresistant organisms. However, are nephrotoxic, and this feature may complicate therapy or even require its discontinuation. Like that aminoglycosides, nephrotoxicity might be related highly cationic nature molecule. Colistin polymyxin B carry five positive charges. Here we show derivatives carrying only three charges...
To evaluate a genome-based surveillance of all Danish third-generation cephalosporin-resistant Escherichia coli (3GC-R Ec ) from bloodstream infections between 2014 and 2015, focusing on horizontally transferable resistance mechanisms.A collection 552 3GC-R isolates were whole-genome sequenced characterized by using the batch uploader Center for Genomic Epidemiology (CGE) automatically analysed CGE tools according to profile, MLST, serotype fimH subtype. Additionally, phylogenetic...
A novel variant of the plasmid-borne colistin resistance gene mcr-3 was detected on an IncHI2 plasmid in ST131 CTX-M-55-producing Escherichia coli isolate from a Danish patient with bloodstream infection 2014. The discovery genes conferring to is special interest since has reemerged as important drug treatment infections multidrug-resistant Gram-negative bacteria.
In enterococci, resistance to linezolid is often mediated by mutations in the V domain of 23S rRNA gene (G2576T or G2505A). Furthermore, four genes [optrA, cfr, cfr(B) and poxtA] encode enterococci. We aimed develop a Web tool for detection two encoding enterococci from whole-genome sequence data. LRE-Finder (where LRE stands linezolid-resistant enterococci) detected fraction Ts position 2576 As 2505 cfr(B), optrA poxtA aligning raw sequencing reads (fastq format) with k-mer alignment. For...
Abstract Objectives The objectives were to study a possible outbreak of carbapenem-resistant Acinetobacter baumannii by comparing three different typing methods (PFGE, MLST and whole-genome SNPs) compare the resistance gene profiles isolates. Methods From December 2012 October 2013, eight A. detected at Odense University Hospital, Odense, Denmark. These isolates typed PFGE, with ApaI SmaI, respectively, subjected WGS. WGS data used for in silico extraction types using two schemes, genes...
An outbreak of NDM-1-producing Citrobacter freundii and possible secondary in vivo spread blaNDM-1 to other Enterobacteriaceae were investigated. From October 2012 March 2015, meropenem-resistant detected 45 samples from seven patients at Aalborg University Hospital, Aalborg, Denmark. In silico resistance genes, Inc plasmid types STs (MLST) obtained WGS data 24 isolates (13 C. freundii, 6 Klebsiella pneumoniae, 4 Escherichia coli 1 oxytoca) meropenem-susceptible K. oxytoca. The sequences the...
Escherichia coli is the most common pathogen causing Urinary Tract Infections (UTI). Data from current National Surveillance program in Denmark (DANMAP) may not accurately represent prevalence of resistant E. primary care, because only urine samples complicated cases be forwarded to microbiological departments at hospitals for diagnostic examination. The aim this study was assess commonly used antimicrobial agents care a consecutive sample patients general practice. Observational carried out...
This study aimed to characterise carbapenemase-producing Acinetobacter baumannii (A. baumannii) isolates from Danish patients using whole genome sequencing (WGS). It also compared typing and epidemiological data for further investigation of the spread origin A. isolates. From 1 January 2014 30 September 2021, 141 isolates, received at national reference laboratory Statens Serum Institut, were investigated WGS. Multilocus sequence (MLST) cgMLST data, obtained by SeqSphere+ software, linked...
This study compares the genome of an ST131 CMY-2-producing Escherichia coli isolate from a Danish patient with other E. isolates both human and animal origin.In 2016, (ESBL20160056) was obtained bloodstream infection. The ESBL20160056 compared genomes six broiler meat imported to Denmark, 15 Enterobase (http://enterobase.warwick.ac.uk) two European collaborators. plasmid sequenced using MinION Mk1B (Oxford Nanopore Technologies).The clustered together 13 fimH22 in distinct clade. clade...
Fully sequenced IncI1 plasmids obtained from CTX-M-1-producing Escherichia coli of human and animal origin were compared.Twelve E. isolates sharing identical ESBL genes plasmid multilocus STs on Illumina MinION platforms the Danish antimicrobial resistance surveillance programme, DANMAP. After de novo assembly, sequences harbouring blaCTX-M-1 manually curated ORFs annotated. Within-group comparisons performed separately for ST3 type ST7 type. The group was 10 (2 patients with bloodstream...
Most Gram-negative community-acquired and nosocomial infections are caused by Escherichia coli Klebsiella pneumoniae, among which increasing resistance due to extended-spectrum β-lactamase (ESBL) is a major problem. We present data from the first Danish nationwide prevalence study on ESBL-producing E. coli, K. Proteus mirabilis in blood urine cultures hospitals community.During September October 2007, 13 of 15 departments clinical microbiology collected strains. Confirmatory ESBL...
ABSTRACT Using Nanopore sequencing, we describe here the circular genome of an Escherichia coli sequence type 410 (ST410) strain with five closed plasmids. A large 111-kb incompatibility group F (IncF) plasmid harbored bla NDM-5 and 16 other resistance genes. 51-kb IncX3 carried QnrS1 OXA-181 . E. isolates both carbapenemases are rare.
Escherichia coli sequence type 131 (ST131) is a major cause of urinary and bloodstream infections. Its association with extended-spectrum β-lactamases (ESBLs) significantly complicates treatment. best-described component the rapidly expanding H30Rx clade, containing allele 30 1 fimbrial adhesin gene fimH This lineage appears to have emerged in United States spread around world part due acquisition ESBL-encoding blaCTX-M-15 resistance fluoroquinolones. However, non-H30 ST131 sublineages other...
The increasing prevalence of ESBL‐, AmpC‐ and carbapenemase‐producing Enterobacteriaceae necessitates reliable phenotypic tests for detection categorization. main objective this study was to evaluate ROSCO Neo‐Sensitabs with different β‐lactam‐β‐lactam inhibitor combinations categorization β‐lactamases in Enterobacteriaceae. Using standard CLSI/EUCAST methodology, differences zones inhibitions between a β‐lactam alone compared the combination β‐lactamase as well subjective synergy...
Background Carbapenemase-producing Escherichia coli are increasing worldwide. In recent years, an increase in OXA-244-producing E. isolates has been seen the national surveillance of carbapenemase-producing organisms Denmark. Aim Molecular characterisation and epidemiological investigation from January 2016 to August 2019. Methods For investigation, data Danish National Patient Registry register civil registration were used together with phone interviews patients. Isolates characterised by...