A5084244691- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial biofilms and quorum sensing
- Pharmaceutical and Antibiotic Environmental Impacts
- Respiratory viral infections research
- Bacterial Identification and Susceptibility Testing
- Escherichia coli research studies
- Antibiotic Use and Resistance
- Genomics and Phylogenetic Studies
- Antimicrobial Resistance in Staphylococcus
- Pneumonia and Respiratory Infections
- Viral gastroenteritis research and epidemiology
- Infections and bacterial resistance
- Bacteriophages and microbial interactions
- Enterobacteriaceae and Cronobacter Research
- Viral Infections and Immunology Research
- Plant Pathogenic Bacteria Studies
- Influenza Virus Research Studies
- Clostridium difficile and Clostridium perfringens research
- Legume Nitrogen Fixing Symbiosis
- Microbial Metabolism and Applications
- Asthma and respiratory diseases
- Mycobacterium research and diagnosis
- Plant-Microbe Interactions and Immunity
University Hospital Cologne
2012-2025
University of Cologne
2016-2025
German Center for Infection Research
2016-2025
Institute of Medical Microbiology and Hygiene
2014-2025
Centrum für Integrierte Onkologie
2022
Princess Margaret Hospital for Children
2022
Perth Children's Hospital
2022
Yamagata Prefectural Central Hospital
2022
Mallinckrodt (United States)
2015-2020
Engineering Associates (United States)
2017
Healthy adult volunteers were inoculated intranasally with human parvovirus obtained from an asymptomatic blood donor. One week after inoculation, intense viremia was observed in seronegative volunteers, accompanied by a mild illness pyrexia, malaise, myalgia, itching, and excretion of virus the respiratory tract. In following hematologic studies revealed reticulocytopenia associated slight drop hemoglobin concentration, lymphopenia, neutropenia, platelet counts. At 17–18 days inoculation...
We have investigated the molecular epidemiology and distribution of carbapenemase genes in 492 imipenem-non-susceptible Acinetobacter baumannii worldwide isolates (North Latin America, Europe, Asia, South Africa Australia). MICs were determined by broth microdilution Etest. The presence carbapenemase-encoding was PCR. Molecular performed repetitive sequence-based PCR (rep-PCR; DiversiLab), sequence-type multiplex PFGE. Imipenem non-susceptibility associated with ISAba1 upstream intrinsic...
We investigated the mechanism of carbapenem resistance in 10 Acinetobacter baumannii strains isolated from United States and Mexico between 2005 2009. The detection known metallo-β-lactamase or carbapenem-hydrolyzing oxacillinase (OXA) genes by PCR was negative. presence plasmid-encoded transformation A. ATCC 17978. Shotgun cloning experiments sequencing were performed, followed expression a novel β-lactamase baumannii. Three OXA enzymes identified, OXA-235 8 isolates amino acid variants...
To investigate the molecular epidemiology, antimicrobial susceptibility and carbapenem resistance determinants of Acinetobacter baumannii isolates from respiratory tract samples patients diagnosed with ventilator-associated pneumonia (VAP) who were enrolled in MagicBullet clinical trial. A. prospectively cultured 65 15 hospitals Greece, Italy Spain. Susceptibility testing was performed by broth microdilution. Carbapenem identified PCR sequencing. Molecular epidemiology investigated using...
ABSTRACT A carbapenem-resistant Acinetobacter baumannii strain was isolated in Brazil 2004 which no known carbapenemase gene detected by PCR. Cloning experiments, followed expression Escherichia coli , gave an E. recombinant expressing a novel carbapenem-hydrolyzing class D β-lactamase (CHDL). OXA-143 showed 88% amino acid sequence identity with OXA-40, 63% OXA-23, and 52% OXA-58. It hydrolyzed penicillins, oxacillin, meropenem, imipenem but not expanded-spectrum cephalosporins. The bla...
To investigate the genetic environment of metallo-β-lactamase gene blaNDM-1 in an Acinetobacter baumannii isolated 2007 a German hospital. Antimicrobial susceptibility testing was performed and resistance genes were characterized by PCR amplification sequencing. Transferability β-lactam tested broth mating assays transformation plasmids. The background analysed primer walking. Typing A. strain repetitive extragenic palindromic sequence-based (rep-PCR) using DiversiLab system....
Infection of normal individuals with human parvovirus (B19) results in a mild disease (erythema infectiosum) but gives rise to aplastic crises patients chronic hemolytic anemias. The effects this on hemopoiesis were investigated following intranasal inoculation the virus into three volunteers. A typical ensued viremia peaking at 9 d. Marrow morphology 6 d after appeared 10 there was severe loss erythroid precursors followed by 1-2-g drop hemoglobin, and an increase serum immunoreactive...
Objectives: 1-(1-Naphthylmethyl)-piperazine (NMP) has been shown to reverse multidrug resistance (MDR) in Escherichia coli overexpressing RND-type efflux pumps but there are no data on its activity non-fermenters like Acinetobacter. Methods: Antimicrobial susceptibility the absence and presence of NMP and, for comparison, phenyl-arginine-β-naphthylamide (PAβN), another putative pump inhibitor (EPI), was tested laboratory mutant strains with differing intracellular dye accumulation expression...
This study aimed to define the taxonomic status of a phenetically distinct group 16 strains that corresponds Acinetobacter genomic species ‘close 13TU’, provisional calcoaceticus – baumannii (ACB) complex recognized by Gerner-Smidt and Tjernberg in 1993. These have been isolated different countries since early 1990s were mostly recovered from human clinical specimens. They compared with 45 reference representing known taxa ACB using methods relevant genus . Based on sequence analysis...
We have employed whole genome sequencing to define and evaluate a core multilocus sequence typing (cgMLST) scheme for Acinetobacter baumannii. To we downloaded total of 1,573 putative A. baumannii genomes from NCBI as well representative isolates belonging the eight previously described international clonal lineages. The was then against fifty-three carbapenem-resistant that were typed by PFGE linked hospital outbreaks in German cities. defined 2,390 genes which an average 98.4% called...
A previously established multiplex PCR that identifies to the species level Acinetobacter baumannii and genomic 13TU (GS13TU) was expanded include calcoaceticus 3.
The recent advances in bacterial species identification methods have led to the rapid taxonomic diversification of genus Acinetobacter. In present study, phenotypic and molecular been used determine position a group 12 genotypically distinct strains belonging Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex, initially described by Gerner-Smidt Tjernberg 1993, that are closely related pittii. Strains characterized this study originated mostly from human samples obtained...
ABSTRACT To give an update on the molecular epidemiology and global distribution of carbapenemase encoding genes, we subjected 313 carbapenem-resistant Acinetobacter baumannii isolated from 114 study centers in 47 countries five world regions, Africa, Asia, Europe, Latin America, North to whole genome sequencing. Numbers isolates investigated were proportional population size contributing countries. Molecular was using seven-loci core multilocus sequence typing, whole-genome single...
The global prevalence of colistin-resistant Klebsiella pneumoniae (ColRkp) facilitated by chromosomal and plasmid-mediated Ara4N or PEtN-remodeled LPS alterations has steadily increased with colistin usage for treating carbapenem-resistant K. (CRkp). Our study demonstrated the rising trend ColRkp showing extensively pandrug-resistant characteristics among CRkp, a 28.5%, which was mediated mgrB, pmrB, phoQ mutations (91.5%), mcr-1.1, mcr-8.1, mcr-8.2 alone in conjunction R256G PmrB (8.5%)....