A5051898439- Chemical Synthesis and Reactions
- Polyamine Metabolism and Applications
- Water Quality Monitoring and Analysis
- Microbial Metabolic Engineering and Bioproduction
- Enzyme-mediated dye degradation
- biodegradable polymer synthesis and properties
- Coenzyme Q10 studies and effects
- Marine Biology and Environmental Chemistry
- Biopolymer Synthesis and Applications
- Environmental Toxicology and Ecotoxicology
- Biofuel production and bioconversion
- Metal-Catalyzed Oxygenation Mechanisms
- Synthesis and Biological Evaluation
- Synthesis and Catalytic Reactions
- Electrochemical sensors and biosensors
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Oxidative Organic Chemistry Reactions
- Enzyme Catalysis and Immobilization
- Adenosine and Purinergic Signaling
- Synthetic Organic Chemistry Methods
- Lipid metabolism and biosynthesis
Technical University of Denmark
2024
University of Pavia
2020-2024
Unspecific peroxygenases (UPOs) are glycosylated fungal enzymes that can selectively oxidize C–H bonds. UPOs employ hydrogen peroxide as the oxygen donor and reductant. With such an easy-to-handle cosubstrate without need for a reducing agent, emerging convenient oxidative biocatalysts. Here, unspecific peroxygenase from Hypoxylon sp. EC38 (HspUPO) was identified in activity-based screen of six putative were heterologously expressed Pichia pastoris. The enzyme found to tolerate selected...
Abstract Alditol oxidases are promising tools for the biocatalytic oxidation of glycerol to more valuable chemicals. By integrating in silico bioprospecting with cell-free protein synthesis and activity screening, an effective pipeline was developed rapidly identify enzymes that active on glycerol. Three thermostable alditol from Actinobacteria Bacterium, Streptomyces thermoviolaceus , Thermostaphylospora chromogena were discovered. The characterization these three flavoenzymes demonstrated...
Cardiac senescence is a typical chronic frailty condition in the elderly population, and cellular aging often associated with oxidative stress. The mitochondrial-membrane flavoenzyme monoamine oxidase A (MAO A) catalyzes deamination of neurotransmitters, its expression increases aged hearts. We produced recombinant human MAO variants at Lys305 that play key role O2 reactivity leading to H2O2 production. K305Q variant as active wild-type enzyme, whereas K305M K305S have 200-fold 100-fold...
Nitrosuccinate is a biosynthetic building block in many microbial pathways. The metabolite produced by dedicated L-aspartate hydroxylases that use NADPH and molecular oxygen as co-substrates. Here, we investigate the mechanism underlying unusual ability of these enzymes to perform successive rounds oxidative modifications. crystal structure Streptomyces sp. V2 N-hydroxylase outlines characteristic helical domain wedged between two dinucleotide-binding domains. Together with FAD, cluster...
Global plastic production exceeded 400 million tons in 2022, urgently demanding improved waste management and recycling strategies for a circular economy. While the enzymatic hydrolysis of polyethylene terephthalate (PET) has become feasible on industrial scales, efficient enzymes targeting other hydrolysable types such as polyurethanes (PURs) are lacking. Recently, amidase signature (AS) family, capable cleaving urethane bonds polyether‐PUR analog linear polyester‐PUR, have been identified....
Global plastic production exceeded 400 million tons in 2022, urgently demanding improved waste management and recycling strategies for a circular economy. While the enzymatic hydrolysis of polyethylene terephthalate (PET) has become feasible on industrial scales, efficient enzymes targeting other hydrolysable types such as polyurethanes (PURs) are lacking. Recently, amidase signature (AS) family, capable cleaving urethane bonds polyether‐PUR analog linear polyester‐PUR, have been identified....