A5009350210- Amino Acid Enzymes and Metabolism
- Enzyme Catalysis and Immobilization
- Photosynthetic Processes and Mechanisms
- Polyamine Metabolism and Applications
- Mycotoxins in Agriculture and Food
- Fungal and yeast genetics research
- Vitamin C and Antioxidants Research
- Plant Gene Expression Analysis
- Carbohydrate Chemistry and Synthesis
- Plant biochemistry and biosynthesis
- Glycosylation and Glycoproteins Research
- Catalysis for Biomass Conversion
- Biofuel production and bioconversion
- Peroxisome Proliferator-Activated Receptors
- Mitochondrial Function and Pathology
- Enzyme Structure and Function
- Plant Pathogens and Fungal Diseases
- Natural Antidiabetic Agents Studies
- Enzyme-mediated dye degradation
University of Pavia
2022-2024
University of Groningen
2021-2024
Photosynthetic organisms, fungi, and animals comprise distinct pathways for vitamin C biosynthesis. Besides this diversity, the final biosynthetic step consistently involves an oxidation reaction carried out by aldonolactone oxidoreductases. Here, we study origin evolution of diversified activities substrate preferences featured these flavoenzymes using molecular phylogeny, kinetics, mutagenesis, crystallographic experiments. We find clear evidence that they share a common ancestor. A...
Patulin synthase (PatE) from Penicillium expansum is a flavin-dependent enzyme that catalyses the last step in biosynthesis of mycotoxin patulin. This secondary metabolite often present fruit and fruit-derived products, causing postharvest losses. The patE gene was expressed Aspergillus niger allowing purification characterization PatE. confirmed PatE active not only on proposed patulin precursor ascladiol but also several aromatic alcohols including 5-hydroxymethylfurfural. By elucidating...
Nitrosuccinate is a biosynthetic building block in many microbial pathways. The metabolite produced by dedicated L-aspartate hydroxylases that use NADPH and molecular oxygen as co-substrates. Here, we investigate the mechanism underlying unusual ability of these enzymes to perform successive rounds oxidative modifications. crystal structure Streptomyces sp. V2 N-hydroxylase outlines characteristic helical domain wedged between two dinucleotide-binding domains. Together with FAD, cluster...
Abstract Discovery of novel enzymes is a challenging task, yet crucial one, due to their increasing relevance as chemical catalysts and biotechnological tools. In our work we present high‐throughput screening approach discovering activities. A screen 96 putative oxidases with 23 substrates led the discovery two new enzymes. The first enzyme, N‐acetyl‐D‐hexosamine oxidase (EC 1.1.3.29) from Ralstonia solanacearum , vanillyl alcohol oxidase‐like flavoprotein displaying highest activity...
Abstract The chemical 5‐hydroxymethylfurfural (HMF) can be derived from lignocellulose and is an interesting bio‐based platform as it has the potential to transformed into numerous valuable building blocks such polymer‐precursor 2,5‐diformylfuran (DFF). To date, only a few oxidases acting on HMF are known by sampling atypical species, we discovered novel flavin‐dependent oxidoreductase honeybee Apis mellifera (beeHMFO). enzyme perform chemoselective oxidation of DFF but also readily accept...
Flavin-dependent carbohydrate oxidases are valuable tools in biotechnological applications due to their high selectivity the oxidation of carbohydrates. In this study, we report biochemical and structural characterization a recently discovered oxidase from bacterium Ralstonia solanacearum, which is member vanillyl alcohol flavoprotein family. Due its exceptionally activity toward N-acetyl-d-galactosamine N-acetyl-d-glucosamine, enzyme was named N-acetyl-glucosamine (NagOx). contrast most...
Abstract Aldaric acids are attractive diacids that can be prepared by selective oxidation of carbohydrates. For this, effective biocatalysts in demand. This work reports on the discovery, biochemical and structural characterization a VAO‐type flavin‐containing carbohydrate oxidase from Citrus sinensis : URAO Cs3 . could overexpressed using prokaryotic eukaryotic expression systems. Extensive revealed enzyme displays high thermostability an exquisite selectivity for uronic acids, galacturonic...