A5019864741- Microplastics and Plastic Pollution
- biodegradable polymer synthesis and properties
- Biofuel production and bioconversion
- Metal-Catalyzed Oxygenation Mechanisms
- Magnetism in coordination complexes
- X-ray Diffraction in Crystallography
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Crystallization and Solubility Studies
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Metal complexes synthesis and properties
- Glycosylation and Glycoproteins Research
- Recycling and Waste Management Techniques
- Effects and risks of endocrine disrupting chemicals
- Enzyme Structure and Function
- Polysaccharides and Plant Cell Walls
- Lanthanide and Transition Metal Complexes
- Crystallography and molecular interactions
- Dental Health and Care Utilization
- Pharmacogenetics and Drug Metabolism
- Oral microbiology and periodontitis research
- Arsenic contamination and mitigation
- Electron Spin Resonance Studies
- Trace Elements in Health
Novozymes (Denmark)
2010-2024
Nawi Graz
2017-2018
University of Graz
2014-2018
University of Copenhagen
2009-2014
University of Southern Denmark
1995-2008
Instituto de Salud del Estado de México
1983
The Sabatier principle states that optimal catalysis occurs when interactions between catalyst and substrate are of intermediary strength. Although qualitative in nature, this concept has proven extremely useful within (nonbiochemical) heterogeneous catalysis. In the current work, we show may be applied to an interfacial enzyme reaction. Specifically, studied breakdown cellulose by different cellulases (wild types variants) found results could rationalized so-called volcano plots emblematic...
Summary Cyanogenic glucosides are present in several crop plants and can pose a significant problem for human animal consumption, because of their ability to release toxic hydrogen cyanide. Sorghum bicolor L. contains the cyanogenic glucoside dhurrin. A qualitative biochemical screen M2 population derived from EMS treatment sorghum seeds, followed by reverse genetic technique Targeted Induced Local Lesions Genomes (TILLING), was employed identify mutants with altered cyanide potential...
The potential of bioprocessing in a circular plastic economy has strongly stimulated research into the enzymatic degradation different synthetic polymers. Particular interest been devoted to commonly used polyester, poly(ethylene terephthalate) (PET), and number PET hydrolases have described. However, kinetic framework for comparisons (or other plastic-degrading enzymes) acting on insoluble substrate not established. Herein, we propose such framework, which tested against measurements four...
Interfacial enzyme reactions are common in Nature and industrial settings, including the enzymatic deconstruction of poly(ethylene terephthalate) (PET) waste. Kinetic descriptions PET hydrolases necessary for both comparative analyses, discussions structure-function relations rational optimization technical processes. We investigated whether Sabatier principle could be used this purpose. Specifically, we compared kinetics two well-known hydrolases, leaf-branch compost cutinase (LCC) a from...
The rate response of poly(ethylene terephthalate) (PET)-hydrolases to increased substrate crystallinity (XC ) PET manifests as a rate-lowering effect that varies significantly for different enzymes. Herein, we report the influence XC on product release six thermostable PET-hydrolases. All enzyme reactions displayed distinctive lag phase until measurable formation occurred. duration with . recently discovered PET-hydrolase PHL7 worked efficiently "amorphous" disks ≈10 %), but this was...
Biocatalytic degradation of plastic waste is anticipated to play an important role in future recycling systems. However, enzymatic crystalline poly (ethylene terephthalate) (PET) remains consistently poor. Herein, we employed functional assays elucidate the molecular underpinnings this limitation. This included utilizing complementary activity monitor PET disks with varying crystallinity (X
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and Reactivity of (.mu.-Oxo)diiron(III) Complexes Tris(2-pyridylmethyl)amine. X-ray Crystal Structures [tpa(OH)FeOFe(H2O)tpa](ClO4)3 [tpa(Cl)FeOFe(Cl)tpa](ClO4)2Alan Hazell, Kenneth B. Jensen, Christine J. McKenzie, Hans ToftlundCite this: Inorg. Chem. 1994, 33, 14, 3127–3134Publication Date (Print):July 1, 1994Publication History Published online1 May 2002Published inissue 1 July...
Arsenic-containing hydrocarbons have been identified for the first time as natural components of fish oil.
In addition to the products of photosynthesis, chloroplast provides energy and carbon building blocks required for synthesis a wealth bioactive natural which many have potential uses as pharmaceuticals. course plant evolution, generation biosynthetic capacities been compartmentalized. Chloroplast photosynthesis ATP NADPH well sources primary metabolism. Cytochrome P450 monooxygenases (P450s) in endoplasmic reticulum (ER) synthesize wide spectrum products, powered by single electron transfers...
Plants are light-driven "green" factories able to synthesize more than 200,000 different bioactive natural products, many of which high-value products used as drugs (e.g., artemisinin, taxol, and thapsigargin). In the formation cytochrome P450 (P450) monooxygenases play a key role in catalyzing regio- stereospecific hydroxylations that often difficult achieve using approaches chemical synthesis. P450-catalyzed monooxygenations dependent on electron donation typically from NADPH catalyzed by...
Cellobiohydrolases are exo-active glycosyl hydrolases that processively convert cellulose to soluble sugars, typically cellobiose. They effectively break down crystalline and make up a major component in industrial enzyme mixtures used for deconstruction of lignocellulosic biomass. Identification the rate-limiting step cellobiohydrolases remains controversial, recent reports have alternately suggested either association (on-rate) or dissociation (off-rate) as overall bottleneck. Obviously,...
Bioprocessing of polyester waste has emerged as a promising tool in the quest for cyclic plastic economy. One key step is enzymatic breakdown polymer, and this entails complicated pathway with substrates, intermediates, products variable size solubility. We have elucidated poly(ethylene terephthalate) (PET) four enzymes. Specifically, we combined different kinetic measurements novel stochastic model found that ability to hydrolyze internal bonds polymer (endo-lytic activity) was parameter...
This study extents the sequence space of 6-aminohexanoate endohydrolases (NylC) for future enzyme engineering strategies to boost their nylonase activities, while in parallel novel polymers were designed align biodegradability and performance.
Iron(II) complexes of the pentadentate ligand N-methyl-N,N′,N′-tris(2-pyridylmethyl)ethane-1,2-diamine (L2) and potentially hexadentate N,N′-bis(6-methyl-2-pyridylmethyl)-N,N′-bis(2-pyridylmethyl)-N,N′-bis(2-pyridylmethyl)ethane-1,2-diamine (L1) have been isolated. Both ligands behave as in their iron(II) with general formulation [FeL(X)]n+(L = L1 or L2, n= 1 2) sixth co-ordination site (X) occupied by an auxiliary e.g. H2O, Cl, SCN CN. The crystal structure [FeL1(H2O)][PF6]2·H2O shows that...
Arsenic-containing lipids (arsenolipids) are novel natural products recently shown to be widespread in marine animals and algae. Research interest these arsenic compounds lies their possible role the membrane chemistry of organisms and, because they occur many popular seafoods, human metabolism toxicology. Progress has been restricted, however, by lack standard arsenolipids a quantitative method for analysis. We report that certified reference material CRM 7405-a (Hijiki) is rich source...
Plants produce an immense variety of specialized metabolites, many which are high value as their bioactive properties make them useful for instance pharmaceuticals. The compounds often produced at low levels in the plant, and due to complex structures, chemical synthesis may not be feasible. Here, we take advantage reducing equivalents generated photosynthesis developing approach producing plant natural a photosynthetic microorganism by functionally coupling biosynthetic enzyme photosystem...
Depending strongly on the ingested selenium (Se) form differences in toxicity, bioavailability as well metabolism occur in<italic>C. elegans</italic>.
Abstract Enzyme reactions, both in Nature and technical applications, commonly occur at the interface of immiscible phases. Nevertheless, stringent descriptions interfacial enzyme catalysis remain sparse, this is partly due to a shortage coherent experimental data guide assess such work. In work, we produced kinetically characterized 83 cellulases, which revealed conspicuous linear free energy relationship (LFER) between substrate binding strength activation barrier. The scaling occurred...
Bioprocessing of poly(ethylene terephthalate) (PET) waste has emerged as a promising approach to circular economy. The key this development been the discovery enzymes that effectively depolymerize PET its constituent monomers, and hitherto, most candidates are quite closely related, bacterial cutinases. Fungal have less investigated, although few examples hydrolytic activity against reported. To further assess this, we produced 24 fungal cutinases homologues known hydrolases identified three...