A5060606282- Photosynthetic Processes and Mechanisms
- Plant biochemistry and biosynthesis
- Plant nutrient uptake and metabolism
- Antioxidant Activity and Oxidative Stress
- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Aldose Reductase and Taurine
- Fluoride Effects and Removal
- Biochemical Acid Research Studies
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Berry genetics and cultivation research
- Plant tissue culture and regeneration
- Plant responses to water stress
- Microbial Community Ecology and Physiology
- Amino Acid Enzymes and Metabolism
- Plant Physiology and Cultivation Studies
- Plant Parasitism and Resistance
- Plant Micronutrient Interactions and Effects
- Fungal and yeast genetics research
- Phytase and its Applications
- Legume Nitrogen Fixing Symbiosis
- Pineapple and bromelain studies
- Enzyme Structure and Function
- Plant Virus Research Studies
University of Chile
2012-2025
University of Cambridge
2001-2006
Transport of nucleotide sugars across the Golgi apparatus membrane is required for luminal synthesis a variety plant cell surface components. We identified an Arabidopsis gene encoding sugar transporter (designated GONST1) that we have shown by transient expression to be localized Golgi. GONST1 complemented GDP-mannose transport–defective yeast mutant (vrg4-2), and Golgi-rich vesicles from strain displayed increased transport activity. promoter::β-glucuronidase studies suggested this...
Plant carotenoids are synthesized and accumulated in plastids through a highly regulated pathway. Lycopene β-cyclase (LCYB) is key enzyme involved directly the synthesis of α-carotene β-carotene cyclization lycopene. Carotenoids produced both carrot (Daucus carota) leaves reserve roots, high amounts accumulate latter. In some plant models, presence different isoforms carotenogenic genes associated with an organ-specific function. D. carota harbors two Lcyb genes, which DcLcyb1 expressed...
Abstract The Arabidopsis thaliana protein GOLGI-LOCALIZED NUCLEOTIDE SUGAR TRANSPORTER (GONST1) has been previously identified as a GDP-d-mannose transporter. It hypothesized that GONST1 provides precursors for the synthesis of cell wall polysaccharides, such glucomannan. Here, we show in vitro can transport all four plant GDP-sugars. However, gonst1 mutants have no reduction glucomannan quantity and detectable alterations other polysaccharides. By contrast, class glycosylated sphingolipids...
Phytoene synthase (PSY) is the first committed enzyme of carotenoid biosynthesis pathway and most important point regulation. Carotenoids are precursors abscisic acid (ABA), which mediates abiotic stress tolerance responses in plants. ABA activates synthesis its own through induction PSY expression. Carrot, a species that accumulates very high amounts carotenoids reserve root, has two paralog genes expressed differentially root. Here, we determined DcPSY2 expression induced by salt ABA. A...
Carotenoids, chlorophylls and gibberellins are derived from the common precursor geranylgeranyl diphosphate (GGPP). One of enzymes in carotenoid biosynthesis is lycopene β-cyclase (LCYB) that catalyzes conversion into β-carotene. In carrot, Dclcyb1 essential for synthesis whole plant. Here we show when expressed tobacco, increments total carotenoids, β-carotene chlorophyll levels occur. Furthermore, photosynthetic efficiency enhanced transgenic lines. Interestingly, contrary to previous...
Aloe barbadensis Miller (Aloe vera) has a Crassulaceae acid metabolism which grants the plant great tolerance to water restrictions. Carbohydrates such as acemannans and fructans are among molecules responsible for tolerating deficit in other species. Nevertheless, fructans, prebiotic compounds, have not been described nor studied vera, whose leaf gel is known possess beneficial pharmaceutical, nutritional cosmetic properties. As vera frequently cultivated semi-arid conditions, like those...
The acquisition of photosynthesis by eukaryotic cells through enslavement a cyanobacterium represents one the most remarkable turning points in history life on Earth. In addition to endosymbiotic gene transfer, protein import apparatus and coordination expression between host endosymbiont genomes, establishment metabolic connection was crucial for functional endosymbiosis. It previously hypothesized that first both partners endosymbiosis achieved insertion host-derived metabolite transporter...
Carotenoids are synthesized in prokaryotic and eukaryotic organisms.In plants algae, these lipophilic molecules possess antioxidant properties acting as reactive oxygen species scavengers exert functional roles hormone synthesis, photosynthesis, photomorphogenesis photoprotection.During the past decade almost all carotenogenic genes have been identified a result of molecular, genetic biochemical approaches utilizing Arabidopsis thaliana model system.Studies carried out leaves fruits A....
Altered starch accumulation is a characteristic biochemical symptom of virus infection in plants. To assess its biological importance, Arabidopsis thaliana with Turnip vein-clearing virus, Cucumber mosaic or Cauliflower was investigated plants grown under continuous illumination (under which there no net breakdown starch) and pgm1 mutant lacking chloroplastic phosphoglucomutase, an enzyme required for biosynthesis. Virus-infected wild-type light exhibited more severe leaf symptoms, but...
Flower colour is mainly due to the accumulation of flavonoids, carotenoids and betalains in petals. Of these pigments, flavonoids are responsible for a wide variety colours ranging from pale yellow (flavones, flavonols flavanodiols) blue-violet (anthocyanins). This character plays crucial ecological role by attracting guiding pollinators. Moreover, ornamental plants market, has been consistently identified as main feature chosen consumers when buying flowers. Considering importance this...
ALFIN-like transcription factors (ALs) are involved in several physiological processes such as seed germination, root development and abiotic stress responses plants. In carrot (Daucus carota), the expression of DcPSY2, a gene encoding phytoene synthase required for carotenoid biosynthesis, is induced after salt abscisic acid (ABA) treatment. Interestingly, DcPSY2 promoter contains multiple ALFIN response elements. By silico analysis, we identified two putative genes with molecular...