A5060634385- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Cellular transport and secretion
- Lipid metabolism and biosynthesis
- Lipid Membrane Structure and Behavior
- Plant nutrient uptake and metabolism
- Endoplasmic Reticulum Stress and Disease
- Pesticide Residue Analysis and Safety
- Autophagy in Disease and Therapy
- Pesticide and Herbicide Environmental Studies
- Plant biochemistry and biosynthesis
- Legume Nitrogen Fixing Symbiosis
- Mass Spectrometry Techniques and Applications
- Polysaccharides and Plant Cell Walls
- Plant tissue culture and regeneration
- Analytical chemistry methods development
- Soybean genetics and cultivation
- Advanced Fluorescence Microscopy Techniques
- Sphingolipid Metabolism and Signaling
- ATP Synthase and ATPases Research
- Fungal Plant Pathogen Control
- Lysosomal Storage Disorders Research
- Algal biology and biofuel production
- Plant-Microbe Interactions and Immunity
Université de Bordeaux
2014-2025
Laboratoire de Biogenèse Membranaire
2016-2025
Centre National de la Recherche Scientifique
2007-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2014-2024
MetaboHUB
2018-2024
Umeå Plant Science Centre
2007-2014
Umeå University
2008-2014
Swedish University of Agricultural Sciences
2007-2013
Institut des Sciences du Végétal
2007
Biologie cellulaire et Cancer
2004
Abstract Plasmodesmata (PD) are nano-sized membrane-lined channels controlling intercellular communication in plants. Although progress has been made identifying PD proteins, the role played by major membrane constituents, such as lipids, defining specialized domains remains unknown. Through a rigorous isolation of “native” fractions and comparative mass spectrometry-based analysis, we demonstrate that lipids laterally segregated along plasma (PM) at cell-to-cell junction Arabidopsis...
Abstract Vacuoles are multifunctional organelles essential for the sessile lifestyle of plants. Despite their central functions in cell growth, storage, and detoxification, knowledge about mechanisms underlying biogenesis associated protein trafficking pathways remains limited. Here, we show that meristematic cells Arabidopsis thaliana root, vacuoles as well sterols two major tonoplast proteins, vacuolar H+-pyrophosphatase H+-adenosinetriphosphatase, occurs independently endoplasmic...
Multiple steps of plant growth and development rely on rapid cell elongation during which secretory endocytic trafficking via the trans -Golgi network (TGN) plays a central role. Here, we identify ECHIDNA (ECH) protein from Arabidopsis thaliana as TGN-localized component crucial for TGN function. ECH partially complements loss budding yeast TVP23 function Populus ech mutant, suggesting functional conservation genes. Compared with wild-type, mutant exhibits severely perturbed well defects in...
The PIN-FORMED (PIN) proteins are plasma-membrane-associated facilitators of auxin transport. They often targeted to one side the cell only through subcellular mechanisms that remain largely unknown. Here, we have studied potential roles cytoskeleton and endomembrane system in localisation PIN proteins. Immunocytochemistry image analysis on root cells from Arabidopsis thaliana maize showed 10-30% intracellular mapped Golgi network, but never prevacuolar compartments. remaining 70-90% were...
Significance Unlike in animals, postembryonic development plants is highly flexible and allows them to modulate their growth patterns response external signals or as part of endogenous developmental programs. Differential cell elongation a widely used program respond signals. Asymmetric distribution the plant hormone indole-acetic acid (auxin) mediated by plasma membrane localized auxin carriers crucial for differential elongation. Our results identify distinct mechanisms trafficking influx...
The post-Golgi compartment trans-Golgi Network (TGN) is a central hub divided into multiple subdomains hosting distinct trafficking pathways, including polar delivery to apical membrane. Lipids such as sphingolipids and sterols have been implicated in from the TGN but underlying mechanisms linking lipid composition functional sorting at remain unknown. Here we demonstrate that with α-hydroxylated acyl-chains of least 24 carbon atoms are enriched secretory vesicle critical for de novo auxin...
Lateral root organogenesis plays an essential role in elaborating plant system architecture. In Arabidopsis , the AP2 family transcription factor PUCHI controls cell proliferation lateral primordia. To identify potential targets of PUCHI, we analyzed a time course transcriptomic dataset formation. We report that multiple genes coding for very long chain fatty acid (VLCFA) biosynthesis enzymes are induced during development PUCHI-dependent manner. Significantly, several mutants perturbed...
Abstract Expansion microscopy (ExM) has revolutionized biological imaging by physically enlarging samples, surpassing the light diffraction limit and enabling nanoscale visualization using standard microscopes. While extensively employed across a wide range of its application to plant tissues is sparse. In this work, we present ROOT-ExM, an expansion method suited for stiff intricate multicellular tissues, focusing on primary root Arabidopsis thaliana. ROOT-ExM achieves isotropic with...
The lipid composition of organelles acts as a landmark to define membrane identity and specify subcellular function. Phosphoinositides are anionic lipids acting in protein sorting trafficking at the trans-Golgi network (TGN). In animal cells, sphingolipids control turnover phosphoinositides through exchange mechanisms endoplasmic reticulum/TGN contact sites. this study, we discover mechanism for how mediate phosphoinositide homeostasis TGN plant cells. Using multiple approaches, show that...
INOSITOL PHOSPHORYLCERAMIDE SYNTHASE 2 (IPCS2) is involved in the biosynthesis of complex sphingolipids at trans-Golgi network (TGN). Here, we demonstrate a role IPCS2 penetration resistance against non-adapted powdery mildew fungi. A novel ipcs2W205* mutant was recovered from forward genetic screen for Arabidopsis plants with enhanced epidermal cell entry success barley fungus Blumeria graminis f. sp. hordei (Bgh). yeast complementation assay and sphingolipidomic approach revealed that...
Root hairs are protrusions from root epidermal cells with crucial roles in plant soil interactions. Although much is known about patterning, polarity and tip growth of hairs, contributions membrane trafficking to hair initiation remain poorly understood. Here, we demonstrate that the trans-Golgi network-localized YPT-INTERACTING PROTEIN 4a 4b (YIP4a/b) contribute activation plasma accumulation Rho-of-plant (ROP) small GTPases during initiation, identifying YIP4a/b as central components...