A5038620590- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Pesticide Residue Analysis and Safety
- Lipid Membrane Structure and Behavior
- Plant Molecular Biology Research
- Lipid metabolism and biosynthesis
- Mass Spectrometry Techniques and Applications
- Analytical chemistry methods development
- Pesticide and Herbicide Environmental Studies
- Autophagy in Disease and Therapy
- Analytical Chemistry and Chromatography
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Soybean genetics and cultivation
- Plant Reproductive Biology
- Metabolomics and Mass Spectrometry Studies
- Calcium signaling and nucleotide metabolism
Laboratoire de Biogenèse Membranaire
2021-2025
Université de Bordeaux
2021-2025
Centre National de la Recherche Scientifique
2021-2025
The lipid composition of organelles acts as a landmark to define membrane identity and specify subcellular function. Phosphoinositides are anionic lipids acting in protein sorting trafficking at the trans-Golgi network (TGN). In animal cells, sphingolipids control turnover phosphoinositides through exchange mechanisms endoplasmic reticulum/TGN contact sites. this study, we discover mechanism for how mediate phosphoinositide homeostasis TGN plant cells. Using multiple approaches, show that...
Abstract The trans-Golgi network (TGN), a key compartment in endomembrane trafficking, participates both secretion to and endocytosis from the plasma membrane. Consequently, TGN plays role plant growth development. Understanding how proteins are sorted for or endocytic recycling at is critical elucidating mechanisms of We previously showed that protein ECHIDNA essential phytohormonal control hypocotyl bending because it mediates cell wall components auxin influx carrier AUXIN RESISTANT 1...
Summary Endoplasmic Reticulum (ER)-to-Golgi trafficking is a central process of the secretory system eukaryotic cells that ensures proper spatiotemporal sorting proteins and lipids 1–5 . However, nature ER-Golgi Intermediate Compartments (ERGIC) molecular mechanisms mediating transition between ERGIC Golgi, as well universality these processes amongst Eukaryotes, remain undiscovered. Here, we took advantage plant cell in which Golgi highly dynamic close vicinity to ER 6–9 We discovered...
Summary Anionic phospholipids (PS, PA, PI, PIPs) are low abundant with impactful functions in cell signaling, membrane trafficking and differentiation processes. They can be quickly metabolized transiently accumulate at define spots within the or an organ to respond physiological environmental stimuli. As even a small change their composition profile will produce significant effect on biological processes, it is crucial develop sensitive optimized analytical method accurately detect quantify...
Anionic phospholipids (PS, PA, PI, PIPs) are low-abundant with impactful functions in cell signaling, membrane trafficking and differentiation processes. They can be quickly metabolized transiently accumulate at defined spots within the or an organ to respond physiological environmental stimuli. As even a small change their composition profile will produce significant effect on biological processes, it is crucial develop sensitive optimized analytical method accurately detect quantify them....
A main characteristic of sphingolipids is the presence a very long chain fatty acid (VLCFA) whose function in cellular processes not yet fully understood. VLCFAs are involved intracellular traffic to vacuole and maturation early endosomes into late one major pathways for vacuolar traffic. Additionally, anionic phospholipid phosphatidylinositol-3-phosphate (PtdIns (3)P or PI3P) protein sorting recruitment small GTPase effectors at endosomes/multivesicular bodies (MVBs) during trafficking. In...
In cells, phospholipids contain acyl chains of variable lengths and saturation, features that affect their functions. Their de novo synthesis in the endoplasmic reticulum takes place via cytidine diphosphate diacylglycerol (CDP-DAG) Kennedy pathways, which are conserved eukaryotes. PA is a key intermediate for all (PI, PIPs, PS, PE, PC, PG CL). The occurs by acylation glycerophosphate leading to 1-acyl lysoPA subsequent at sn-2 position. Using membranes from Escherichia coli overexpressing...