A5023537762- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant responses to water stress
- Insect-Plant Interactions and Control
- Silicon Effects in Agriculture
- Plant Parasitism and Resistance
- Lipid metabolism and biosynthesis
- Plant and animal studies
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Rice Cultivation and Yield Improvement
- Light effects on plants
- Advanced MRI Techniques and Applications
- Advanced Neuroimaging Techniques and Applications
- Legume Nitrogen Fixing Symbiosis
- Functional Brain Connectivity Studies
- Historical Studies and Socio-cultural Analysis
- Gene Regulatory Network Analysis
- Plant Gene Expression Analysis
- Plant Pathogens and Fungal Diseases
- Heat shock proteins research
- Plant Physiology and Cultivation Studies
Université de Montpellier
2012-2024
Institut de Recherche pour le Développement
2012-2024
Diversité, adaptation et développement des plantes
2012-2024
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2021-2024
Resonance Research (United States)
2024
Stanford University
2024
University of Minnesota
2024
Centre National de la Recherche Scientifique
2004-2023
Centre d'Etude Regional pour l'Amelioration de l'Adaptation a la Secheresse
2023
UMR Botanique et Modélisation de l’Architecture des Plantes et des végétations
2023
A striking phenomenon unique to the kingdom of plants is regular arrangement lateral organs around a central axis, known as phyllotaxis. Recent molecular-genetic experiments indicate that active transport plant hormone auxin key process regulating conceptual model based on these experiments, introduced by Reinhardt et al. [Reinhardt, D., Pesce, E. R., Stieger, P., Mandel, T., Baltensperger, K., (2003) Nature 426, 255–260], provides an intuitively plausible interpretation data, but raises...
In Arabidopsis , lateral root primordia (LRPs) originate from pericycle cells located deep within the parental and have to emerge through endodermal, cortical, epidermal tissues. These overlaying tissues place biomechanical constraints on LRPs that are likely impact their morphogenesis. This study probes interplay between patterns of cell division, organ shape, LRP morphogenesis by exploiting recent advances in live plant imaging image analysis. Our 3D/4D analysis revealed early stage...
One of the most striking features plant architecture is regular arrangement leaves and flowers around stem, known as phyllotaxis. Peaks in concentration hormone auxin, generated by polar localization PIN1 auxin efflux carrier, provide instructive signal for primordium initiation. This mechanism generates spacing between neighboring primordia, which results Studies role transport phyllotactic patterning have focused on PIN1-mediated efflux. Recent computer simulations indicate an additional...
Abstract Activated forms of jasmonic acid (JA) are central signals coordinating plant responses to stresses, yet tools analyse their spatial and temporal distribution lacking. Here we describe a JA perception biosensor termed Jas9-VENUS that allows the quantification dynamic changes in response stress with high spatiotemporal sensitivity. We show abundance is dependent on bioactive isoforms, COI1 co-receptor, functional Jas motif proteasome activity. demonstrate utility planta at cellular...
Pearl millet plays an important role for food security in arid regions of Africa and India. Nevertheless, it is considered orphan crop as lags far behind other cereals terms genetic improvement efforts. Breeding pearl varieties with improved root traits promises to deliver benefits water nutrient acquisition. Here, we characterize early system development using several different phenotyping approaches that include rhizotrons microCT. We report stage characterized by a fast growing primary...
Abstract A large number of genes involved in lateral root (LR) organogenesis have been identified over the last decade using forward and reverse genetic approaches Arabidopsis thaliana. Nevertheless, how these interact to form a LR regulatory network largely remains be elucidated. In this study, we developed time-delay correlation algorithm (TDCor) infer gene (GRN) controlling primordium initiation patterning from time-series transcriptomic data set. The predicted topology links very...
Lateral root organogenesis plays an essential role in elaborating plant system architecture. In Arabidopsis , the AP2 family transcription factor PUCHI controls cell proliferation lateral primordia. To identify potential targets of PUCHI, we analyzed a time course transcriptomic dataset formation. We report that multiple genes coding for very long chain fatty acid (VLCFA) biosynthesis enzymes are induced during development PUCHI-dependent manner. Significantly, several mutants perturbed...
Lateral root (LR) formation is an important determinant of system architecture. In Arabidopsis, LRs originate from pericycle cells, which undergo a programme morphogenesis to generate new LR meristem. Despite its importance for meristem organisation, the onset organizing center (termed quiescent center; QC) during remains unclear. Here, we used live 3D confocal imaging monitor cell organization and identity acquisition development. Our dynamic observations revealed early phase late as...
Root system architecture plays an important role in determining nutrient and water acquisition is modulated by endogenous environmental factors, resulting considerable developmental plasticity. The orientation of primary root growth response to gravity (gravitropism) has been studied extensively, but little known about the behaviour lateral roots this signal. Here, we analysed and, consistently with previous observations, showed that gravitropism was acquired slowly after emergence. Using a...
Recent progress in root phenotyping has focused mainly on increasing throughput for genetic studies, while identifying developmental patterns been comparatively underexplored. We introduce a new pipeline producing high-quality spatiotemporal system development data and within these data. The SmartRoot image-analysis temporal spatial statistical models were applied to two cereals, pearl millet (Pennisetum glaucum) maize (Zea mays). Semi-Markov switching linear used cluster lateral roots based...
Seedling root traits impact plant establishment under challenging environments. Pearl millet is one of the most heat and drought tolerant cereal crops that provides a vital food source across sub-Saharan Sahel region. millet’s early system features single fast-growing primary which we hypothesize an adaptation to Sahelian climate. Using crop modeling, demonstrate stress important constraint in agrosystems where pearl was domesticated. Furthermore, show increased growth correlated with water...
Lateral root organogenesis is a key process in the development of plant's system and its adaptation to environment. During lateral formation, an early phase cell proliferation first produces four-cell-layered primordium, only from this stage onwards meristem-like structure, expressing stem niche marker genes, being established developing organ. Previous studies reported that gene regulatory network controlling formation organized into two subnetworks whose mutual inhibition may contribute...
In order to understand the functioning of apical meristems in Arabidopsis more clearly, a new mutant, mgoun3 (mgo3), affected structural organization and functional regulation both shoot root has been isolated. mgo3 plants display perturbations leaf morphogenesis, spatial temporal formation primordia, frequent fasciation inflorescence stem. Cellular analysis showed that cellular cell identity patterning are impaired mutant meristems. The MGO3 gene isolated by positional cloning. protein...
SUMMARY In cereals, the root system is mainly composed of post‐embryonic shoot‐borne roots, named crown roots. The CROWN ROOTLESS1 (CRL1) transcription factor, belonging to ASYMMETRIC LEAVES2‐LIKE/LATERAL ORGAN BOUNDARIES DOMAIN (ASL/LBD) family, a key regulator initiation in rice ( Oryza sativa ). Here, we show that CRL1 can bind, both vitro and vivo , not only LBD‐box DNA sequence recognized by several ASL/LBD factors, but also another previously identified motif was CRL1‐box . Using...
COI1-mediated perception of jasmonate is critical for plant development and responses to environmental stresses. Monocots such as rice have two groups COI genes due gene duplication: OsCOI1a OsCOI1b that are functionally equivalent the dicotyledons COI1 OsCOI2 whose function remains unclear. In order assess its functional redundancy with genes, we developed a series mutants in 3 , by CRISPR Cas9-mediated editing characterized their phenotype jasmonate. Characterization uncovered important...
Abstract The complex and adaptable architecture of the plant root system in soil is paramount importance for crop growth performance. Root depends on activity apical meristem, an organised population proliferating progenitor cells continuously replenished from a stem cell niche. branching, which greatly contributes to most dicot species, consists de novo formation new meristems existing tissues. This phenomenon illustrates ability plants repeatedly generate tissues specialised post‐embryonic...
Summary Crown roots (CRs) are essential components of the rice root system. Several genes involved in CR initiation or development have been identified but our knowledge about how they organize to form a gene regulatory network (GRN) is still limited. To characterize cascades acting during formation, we used systems biology approach infer GRN controlling formation downstream CROWN ROOTLESS 1 ( CRL1 ), coding for an ASL (asymmetric leaves‐2‐like)/LBD (LOB domain) transcription factor...