A5034692233- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Nematode management and characterization studies
- Agronomic Practices and Intercropping Systems
- RNA Research and Splicing
- Neurological diseases and metabolism
- Spondyloarthritis Studies and Treatments
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Hereditary Neurological Disorders
- Genetic Associations and Epidemiology
- Psoriasis: Treatment and Pathogenesis
- RNA modifications and cancer
- Plant-Microbe Interactions and Immunity
- Genetics and Neurodevelopmental Disorders
- Cancer-related molecular mechanisms research
- Rheumatoid Arthritis Research and Therapies
- Plant and Fungal Interactions Research
- Biochemical and biochemical processes
- Genetic Syndromes and Imprinting
- Fermentation and Sensory Analysis
- Neurogenetic and Muscular Disorders Research
- Biofuel production and bioconversion
Centre National de la Recherche Scientifique
2005-2024
Université Paris-Saclay
2005-2024
Institut des Sciences des Plantes de Paris Saclay
2010-2024
Université Paris Cité
2019-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2024
Centre National de Recherche en Génomique Humaine
2006-2023
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2008-2023
CEA Paris-Saclay
2020-2023
Direction de la Recherche Fondamentale
2021
Sorbonne Paris Cité
2020
Abstract As sessile organisms, plants are continuously exposed to heterogeneous and changing environments constantly need adapt their growth strategies. They have evolved complex mechanisms recognize various stress factors, activate appropriate signaling pathways, respond accordingly by reprogramming the expression of multiple genes at transcriptional, post-transcriptional, even epigenome levels tolerate stressful conditions such as drought, high temperature, nutrient deficiency, pathogenic...
We examined the timing and location of several early root responses to Rhizobium leguminosarum bv. trifolii infection, compared with a localized addition cytokinin in white clover, study role signaling during nodule initiation. Induction ENOD40 expression by either rhizobia or was similar occurred progenitor cells inner cortex. Inoculation mature failed induce cortical cell divisions (ccd). Nitrate at levels repressing formation inhibited induction but not cytokinin. induced auxin, an auxin...
Under nitrogen-limiting conditions Rhizobium meliloti can establish symbiosis with Medicago plants to form nitrogen-fixing root nodules. Nodule organogenesis starts the dedifferentiation and division of cortical cells. In these cells early nodulin gene enod40, which encodes an unusually small peptide (12 or 13 amino acids), is induced from beginning this process. Herein we show that enod40 expression evokes nodule initiation. (i) Nitrogen-deprived transgenic truncatula overexpressing exhibit...
Though numerous pieces of evidence point to major physiological roles for anion channels in plants, progress the understanding their biological functions is limited by small number genes identified so far. Seven chloride channel (CLC) members could be Arabidopsis genome, amongst which AtCLCe and AtCLCf are both more closely related bacterial CLCs than other plant CLCs. It shown here that targeted thylakoid membranes chloroplasts and, agreement with this subcellular localization, clce mutants...
Molecular mechanisms involved in the control of root nodule organogenesis plant host are poorly understood. One nodulin genes associated with earliest phases this developmental program is enod40. We show here that transgenic Medicago truncatula plants overexpressing enod40 exhibit accelerated nodulation induced by Sinorhizobium meliloti. This resulted from increased initiation primordia, which was accompanied a proliferation response region close to tip and enhanced length. The cortex...
Systematic sequencing of expressed sequence tags (ESTs) can give a global picture the assembly genes involved in development and function organs. Indeterminate nodules representing different stages developmental program are especially suited to study organogenesis. With vector λHybriZAP, cDNA library was constructed from emerging Medicago truncatula induced by Sinorhizobium meliloti. The 5′ ends 389 clones were sequenced, then these ESTs analyzed both homology search studying their...
A diversity of mRNAs containing only short open reading frames (sORF-RNAs; encoding less than 30 amino acids) have been shown to be induced in growth and differentiation processes. The early nodulin gene enod40, coding for a 0.7-kb sORF-RNA, is expressed the nodule primordium developing root cortex leguminous plants after infection by symbiotic bacteria. Ballistic microtargeting this into Medicago roots division cortical cells. Translation two sORFs (I II, 13 27 acids, respectively) present...
Nuclear speckle RNA binding proteins (NSRs) act as regulators of alternative splicing (AS) and auxin-regulated developmental processes such lateral root formation in Arabidopsis thaliana. These were shown to interact with specific alternatively spliced mRNA targets at least one structured lncRNA, named Alternative Splicing Competitor RNA. Here, we used genome-wide analysis RNAseq monitor the NSR global role on multiple tiers gene expression, including processing AS. NSRs affect AS 100s genes...
Monosymmetry evolved several times independently during flower evolution. In snapdragon (Antirrhinum majus), a key gene for monosymmetry is CYCLOIDEA (CYC), which belongs to the class II TCP family encoding transcriptional activators. We address questions of evolutionary history this and possible recruitment genes homologous CYC in floral development symmetry Papaveraceae. Two three members were found each species analyzed, two CYC-like genes, on basis presence both R conserved domains. The...
Abstract Fertility quantitative trait loci (QTL) are of high interest in dairy cattle since insemination failure has dramatically increased some breeds such as Holstein. High-throughput SNP analysis and microarrays give the opportunity to genotype many animals for hundreds SNPs per chromosome. In this study, due these techniques a dense marker map was used fine QTL underlying nonreturn rate measured 90 days after artificial previously detected with low-density microsatellite map. A...
Spondyloarthritis (SpA) is a chronic inflammatory disorder with strong genetic predisposition dominated by the role of HLA-B27. However, contribution other genes to disease susceptibility has been clearly demonstrated. We previously reported significant evidence linkage SpA chromosome 9q31–34. The current study aimed characterize this locus, named SPA2. First, we performed fine mapping SPA2 (24 cM) 28 microsatellite markers in 149 multiplex families, which allowed us reduce area...
Abstract Despite considerable progress in our knowledge regarding the cell cycle inhibitor of Kip-related protein (KRP) family plants, less is known about coordination endoreduplication and differentiation. In animals, role cyclin-dependent kinase (CDK) inhibitors as multifunctional factors coordinating regulation differentiation well documented involves not only inhibition CDK/cyclin complexes but also other mechanisms, among them transcription. Interestingly, several plant KRPs have a...
Abstract Pure hereditary spastic paraplegias are characterized by isolated and progressive spasticity in the lower limbs. We mapped paraplegia 28 ( SPG28 ) locus to chromosome 14q21.3‐q22.3 a Moroccan family with autosomal recessive paraplegia. Affected patients experienced development of gait during childhood required help walking their early 40s. Nine additional families were not linked this locus, demonstrating further genetic heterogeneity. No mutations found exons GCH1 SPG3A , two genes...
Because regulation of its activity is instrumental either to support cell proliferation and growth or promote death, the universal myo-inositol phosphate synthase (MIPS), responsible for biosynthesis, a critical enzyme primary metabolism. Surprisingly, we found this be imported in nucleus interact with histone methyltransferases ATXR5 ATXR6, raising question whether MIPS1 has function transcriptional regulation. Here, demonstrate that binds directly promoter stimulate own expression by...
The mutualistic symbiosis between flowering plants and arbuscular mycorrhizal fungi is extremely abundant in terrestrial ecosystems. In this symbiosis, obligately biotrophic colonize the root of host plants, which can benefit from these by enhanced access to mineral nutrients soil, especially phosphorus. One main goals research on find plant genes that control fungal development plant. work, we show colonization regulated enod40 , an early nodulin gene known be involved nodule legumes with...
The human condition known as Premature Ovarian Failure (POF) is characterized by loss of ovarian function before the age 40. A majority POF cases are sporadic, but 10-15% familial, suggesting a genetic origin disease. Although several causal mutations have been identified, etiology still unknown for about 90% patients.We report genome-wide linkage and homozygosity analysis in one large consanguineous Middle-Eastern POF-affected family presenting an autosomal recessive pattern inheritance. We...
<b><i>Background:</i></b><i>TFAP2B</i> rs987237 is associated with obesity and has shown interaction the dietary fat-to-carbohydrate ratio, which an effect on weight loss. We investigated interactions between protein-to-carbohydrate ratio or glycemic index (GI) in relation to maintenance after <b><i>Methods:</i></b> This study included 742 obese individuals from 8 European countries who participated Diet, Obesity, Genes (DiOGenes)...