A5023216693- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
- Epigenetics and DNA Methylation
- Genetic Syndromes and Imprinting
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- RNA Interference and Gene Delivery
- Plant and animal studies
- Themes in Literature Analysis
- Genomics and Phylogenetic Studies
- Prenatal Screening and Diagnostics
- Rangeland Management and Livestock Ecology
- Immune Cell Function and Interaction
- Cancer therapeutics and mechanisms
- Soybean genetics and cultivation
- Synthesis and Biological Evaluation
- Radiation Effects and Dosimetry
- Gene expression and cancer classification
- Agriculture Sustainability and Environmental Impact
- Plant responses to water stress
University of Bern
2018-2023
ETH Zurich
2016-2021
École Polytechnique Fédérale de Lausanne
2021
University of Tübingen
2008-2019
Institute of Plant Biology
2019
National University of Singapore
2006-2012
Temasek Life Sciences Laboratory
2006-2012
Imprinted genes are expressed predominantly from either their paternal or maternal allele. To date, all imprinted identified in plants the endosperm. In Arabidopsis thaliana, imprinting has been clearly demonstrated for Polycomb group gene MEDEA (MEA) and FWA. Direct repeats upstream of FWA subject to DNA methylation. However, it is still not clear what extent similar cis-acting elements may be part a conserved molecular mechanism controlling maternally genes. this work, we show that...
Parental genomic imprinting causes preferential expression of one the two parental alleles. In mammals, differential sex-dependent deposition silencing DNA methylation marks during gametogenesis initiates a new cycle imprinting. has been detected in plants and relies on by methyltransferase MET1. However, contrast to plant imprints are created removal gametogenesis. Arabidopsis, demethylation is mediated glycosylase DEMETER (DME) causing activation imprinted genes at end female On basis...
Abstract Double fertilization of the female gametophyte produces endosperm and embryo enclosed in maternal seed coat. Proper communication necessitates exchanges signals between zygotic components seed. However, nature these interactions remains largely unknown. We show that double Arabidopsis thaliana rapidly triggers sustained cell proliferation Cell differentiation coat occur autonomous seeds produced absence multicopy suppressor ira1 (msi1) mutant. As msi1 mostly contain endosperm, our...
In mammals and in plants, parental genome dosage imbalance deregulates embryo growth might be involved reproductive isolation between emerging new species. Increased of maternal genomes represses while an increased paternal has the opposite effect. These observations led to discovery imprinted genes, which are expressed by a single allele. It was further proposed frame conflict theory that imbalances directly mirrored antagonistic regulations genes encoding inhibitors enhancers. However...
Plant RNA silencing operates via RNA-directed DNA-methylation (RdDM) to repress transcription or by targeting mRNAs posttranscriptional gene (PTGS). These pathways rely on distinct Dicer-like (DCL) proteins that process double-stranded (dsRNA) into small-interfering RNAs (siRNAs). Here, we explored the expression and subcellular localization of Arabidopsis thaliana DCL4. DCL4 predominates as a start site isoform encoding cytoplasmic protein, which also represents ancestral form in plants. A...
Abstract Animal and plant microRNAs (miRNAs) are essential for the spatio-temporal regulation of development. Together with this role, miRNAs have been proposed to target transposable elements (TEs) stimulate production epigenetically active small interfering RNAs. This activity is evident in male gamete containing structure, gametophyte or pollen grain. How dual role miRNAs, regulating both genes TEs, integrated during development which mRNAs regulated by cell type at a genome-wide scale...
SUMMARY Arabidopsis encodes 10 ARGONAUTE (AGO) effectors of RNA silencing, canonically loaded with either 21–22 nucleotide (nt) long small RNAs (sRNAs) to mediate post‐transcriptional gene silencing (PTGS) or 24 nt sRNAs promote RNA‐directed DNA methylation. Using full‐locus constructs, we characterized the expression, biochemical properties and possible modes action AGO3. Although AGO3 arose from a recent duplication at AGO2 locus, their expression patterns differ drastically, being...
Small RNAs play an important role in regulating gene expression through transcriptional and post-transcriptional silencing. Biogenesis of small from longer double-stranded (ds) RNA requires the activity dicer-like ribonucleases (DCLs), which plants are aided by dsRNA binding proteins (DRBs). To gain insight into this pathway model plant Arabidopsis , we searched for interactors DRB4 immunoprecipitation followed mass spectrometry-based fingerprinting discovered DRB7.1. This interaction,...
Since the finishing of sequencing Arabidopsis thaliana genome, community and annotator centers have been working on improvement gene annotation at structural functional levels. In this context, we used large CATMA resource transcriptome to search for genes missed by different processes. Probes microarrays are specific sequence tags (GSTs) based CDS models predicted Eugene software. Among 24 576 v2 GSTs, 677 in regions considered as intergenic TAIR annotation. We analyzed cognate data carried...
Abstract Complex epigenetic changes occur during plant reproduction. These regulations ensure the proper transmission of information as well allowing for zygotic totipotency. In Arabidopsis , main DNA methyltransferase is called MET1 and responsible methylating cytosine in CG context. The genome encodes three additional reproduction-specific homologs namely MET2a MET2b MET3. this paper, we show that MET3 expressed seed endosperm its expression later restricted to chalazal endosperm....
A bstract During sexual reproduction, development of a totipotent zygote from the fusion highly differentiated gametes is accompanied by dynamic regulation gene expression. This notably involves RNA silencing operated Argonautes (AGO) effector proteins. While AGOs’ roles during Arabidopsis somatic life have been extensively investigated, less known about their expression which proceeds via double-fertilization an egg and central cell, leading respectively to embryo supportive tissue as...
DNA methylation is an important epigenetic mark required for proper gene expression and silencing of transposable elements. patterns can be modified by environmental factors such as pathogen infection, in which modification associated with plant resistance. To counter the defense pathways, pathogens produce effector molecules, several act proteasome inhibitors. Here, we investigated effect inhibition bacterial virulence factor syringolin A (SylA) on genome-wide methylation. We show that SylA...