A5010701186- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Crop Yield and Soil Fertility
- Chromosomal and Genetic Variations
- Polysaccharides and Plant Cell Walls
- Legume Nitrogen Fixing Symbiosis
- Plant Taxonomy and Phylogenetics
- Plant and Biological Electrophysiology Studies
- Plant responses to water stress
- Genetic Syndromes and Imprinting
- Plant Genetic and Mutation Studies
- Gene expression and cancer classification
- Photosynthetic Processes and Mechanisms
- Seed Germination and Physiology
- Nematode management and characterization studies
- Plant-Microbe Interactions and Immunity
- Molecular Biology Techniques and Applications
- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Plant and fungal interactions
- Botany and Plant Ecology Studies
- Tree Root and Stability Studies
- Seedling growth and survival studies
Institut de Recherche pour le Développement
2005-2023
Diversité, adaptation et développement des plantes
2008-2023
Agropolis International
2008-2023
Institut de Recherche pour le Développement
2005-2023
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2020-2022
Université de Montpellier
2013-2022
Centre National de la Recherche Scientifique
2010-2021
Laboratoire Reproduction et Développement des Plantes
2021
École Normale Supérieure de Lyon
2021
Institut national de recherche en informatique et en automatique
2021
Root endosymbioses vitally contribute to plant nutrition and fitness worldwide. Nitrogen-fixing root nodulation, confined four orders, encompasses two distinct types of associations, the interaction legumes (Fabales) with rhizobia bacteria actinorhizal symbioses, where bacterial symbionts are actinomycetes genus Frankia . Although several genetic components host–symbiont have been identified in legumes, basis actinorhiza formation is unknown. Here, we show that receptor-like kinase gene...
Abstract Whether deposited maternal products are important during early seed development in flowering plants remains controversial. Here, we show that RNA interference–mediated downregulation of transcription is deleterious to endosperm but does not block zygotic divisions. Furthermore, POLYMERASE II less active the embryo than endosperm. This dimorphic pattern established late female gametogenesis and inherited by two fertilization. juxtaposition distinct transcriptional activities...
Cytosine methylation is a key epigenetic mark in many organisms, important for both transcriptional control and genome integrity. While relatively stable during somatic growth, DNA reprogrammed genome-wide mammalian reproduction. Reprogramming essential zygotic totipotency to prevent transgenerational inheritance of epimutations. However, the extent reprogramming plants remains unclear. Here, we developed sensors reporting with single-cell resolution CG non-CG Arabidopsis. Live imaging...
Abstract Introducing asexual reproduction through seeds – apomixis into crop species could revolutionize agriculture by allowing F1 hybrids with enhanced yield and stability to be clonally propagated. Engineering synthetic has proven feasible in inbred rice the inactivation of three genes ( MiMe ), which results conversion meiosis mitosis a line ectopically expressing BABYBOOM1 BBM1 ) parthenogenetic trigger egg cells. However, only 10–30% are clonal. Here, we show that can achieved an...
In multicellular organisms, sexual reproduction requires the separation of germline from soma. flowering plants, female precursor differentiates as a single spore mother cell (SMC) ovule primordium forms. Here, we explored how organ growth contributes to SMC differentiation. We generated 92 annotated 3D images at cellular resolution in Arabidopsis. identified spatio-temporal pattern division that acts domain-specific manner Tissue models uncovered plausible morphogenetic principles involving...
Summary T‐DNA integration in the nuclear plant genome may lead to rearrangements of target site. Here we present evidence for a chromosomal inversion 26 cM bordered by two T‐DNAs direct orientation, which is linked mgoun2 mutation. The sites map at positions 80 and 106 chromosome I show that each sequences from 106, respectively. Although are physically distant, they genetically closely linked. In addition, three markers located on segment between no recombination with mgo2 We cannot be...
In recent years, RNA interference has been exploited as a tool for investigating gene function in plants. We tested the potential of double-stranded technology silencing transgene actinorhizal tree Allocasuarina verticillata. The approach was undertaken using stably transformed shoots expressing beta-glucuronidase (GUS) under control constitutive promoter 35S; were further with Agrobacterium rhizogenes A4RS containing hairpin (hpRNA) directed toward GUS gene, and driven by 35S promoter....
In flowering plants, germline precursors are differentiated from somatic cells. The female precursor of Arabidopsis thaliana is located in the internal (nucellar) tissue ovule, and known as Megaspore Mother Cell (MMC). MMC differentiation occurs when a cell subepidermal layer nucellar apex enters meiotic program. Increasing evidence has demonstrated that specification plastic process where number developmental outcome MMCs variable. During its differentiation, displays specific chromatin...
During embryogenesis there is a major switch from dependence upon maternally-deposited products to reliance on of the zygotic genome. In animals, this so-called maternal-to-zygotic transition occurs following period transcriptional quiescence. Recently, we have shown that early embryo in Arabidopsis also quiescent, state inherited female gamete and linked specific patterns H3K9 dimethylation TERMINAL FLOWER2 (TFL2) localization. We demonstrated CHROMOMETHYLASE 3 (CMT3) required for egg cell...
In plants and animals, embryo development becomes ultimately controlled by zygotic genes, but the timing of genome activation (ZGA) varies greatly between organisms. We recently showed that transcriptome young Arabidopsis embryos is dominated maternal transcripts with a progressive ZGA under control epigenetic pathways. contrast, another study reported both parental genomes contribute equally to embryos, suggesting occurs immediately after fertilization. How explain such dramatic...
Introduction Differentiation of spore mother cells marks the somatic-to-reproductive transition in higher plants. Spore are critical for fitness because they differentiate into gametes, leading to fertilization and seed formation. The female cell is called megaspore (MMC) specified ovule primordium. number MMCs varies by species genetic background, but most cases, only a single mature MMC enters meiosis form embryo sac. Multiple candidate precursor have been identified both rice Arabidopsis...
Microarray experiments allow RNA level measurements for many genes in multiple samples. However, mining the biological information from large sets of data generated by microarrays requires use appropriate statistical methods to adjust observed values experimentally introduced variability (normalization process) before testing differences among Normalization microarray is a critical step reducing false positives and negatives. This paper explores normalization cDNA method that uses blank spot...
Summary In multicellular organisms, sexual reproduction requires the separation of germline from soma. flowering plants, first cells germline, so-called spore mother (SMCs), differentiate as reproductive organs form. Here, we explored how organ growth influences and contributes to SMC differentiation. We generated a collection 92 annotated 3D images capturing ovule primordium ontogeny at cellular resolution in Arabidopsis. identified spatio-temporal pattern cell divisions that acts...