A5000403310- Chromosomal and Genetic Variations
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- Plant Disease Resistance and Genetics
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Korean Peninsula Historical and Political Studies
- Plant Molecular Biology Research
- Plant Genetic and Mutation Studies
- Genetic Syndromes and Imprinting
- Virus-based gene therapy research
- Genetic and Environmental Crop Studies
- Enterobacteriaceae and Cronobacter Research
- RNA Research and Splicing
- Fungal Infections and Studies
- Plant responses to water stress
- Nematode management and characterization studies
- Genomics and Phylogenetic Studies
Cold Spring Harbor Laboratory
2012-2024
Howard Hughes Medical Institute
2024
Max Delbrück Center
2007-2023
Universität Hamburg
2014
Cytosine methylation is a key epigenetic mark in many organisms, important for both transcriptional control and genome integrity. While relatively stable during somatic growth, DNA reprogrammed genome-wide mammalian reproduction. Reprogramming essential zygotic totipotency to prevent transgenerational inheritance of epimutations. However, the extent reprogramming plants remains unclear. Here, we developed sensors reporting with single-cell resolution CG non-CG Arabidopsis. Live imaging...
The Sleeping Beauty (SB) transposable element shows efficient transposition in human cells, and provides long-term transgene expression preclinical animal models. Random chromosomal insertion of SB vectors represents a safety issue gene therapeutic applications, due to potential genotoxic effects associated with transposon integration. We investigated the transcriptional activities order assess its alter host upon untranslated regions (UTRs) direct convergent, inward-directed transcription....
In Arabidopsis , LTR retrotransposons are activated by mutations in the chromatin gene DECREASE DNA METHYLATION 1 ( DDM1 ), giving rise to 21- 22-nt epigenetically siRNA (easiRNA) that depend on RNA DEPENDENT POLYMERASE 6 (RDR6). We purified virus-like particles (VLPs) from ddm1 and ddm1rdr6 mutants which genomic is reverse transcribed into complementary DNA. High-throughput short-read long-read sequencing of VLP (VLP DNA-seq) revealed a comprehensive catalog active without need for mapping...
Abstract RNA interference is required for post-transcriptional silencing, but also has additional roles in transcriptional silencing of centromeres and genome stability. However, these have been controversial mammals. Strikingly, we found that Dicer-deficient embryonic stem cells strong proliferation chromosome segregation defects as well increased transcription centromeric satellite repeats, which triggers the interferon response. We conducted a CRISPR-Cas9 genetic screen to restore...
ABSTRACT Populations of genetically identical Sinorhizobium fredii NGR234 cells differ significantly in their expression profiles autoinducer (AI)-dependent and AI-independent genes. Promoter fusions the AI synthase genes traI ngrI showed high levels phenotypic heterogeneity during growth TY medium on a single-cell level. However, adding very concentrations N -(3-oxooctanoyl-)- l -homoserine lactone resulted more homogeneous profile. Similarly, lack internally synthesized AIs background...
Epigenetic modifications that arise during plant and animal development, such as DNA histone modification, are mostly reset gamete formation, but some inherited from the germline including those marking imprinted genes
Transposons are parasitic genetic elements that frequently hijack vital cellular processes of their host. HMGXB4 is a known Wnt signaling-regulating HMG-box protein, previously identified as host-encoded factor Sleeping Beauty (SB) transposition. Here, we show predominantly maternally expressed, and marks both germinal progenitor somatic stem cells. SB piggybacks to activate transposase expression target transposition cells, thereby potentiating heritable transposon insertions. The promoter...
Abstract Transposons are parasitic genetic elements that frequently hijack key cellular processes of the host. HMGXB4 is a Wnt signalling-associated HMG-box protein, previously identified as transcriptional regulating host factor Sleeping Beauty (SB) transposition. Here, we establish highly expressed from zygote stage, and declines after genome activation. Nevertheless, activated by its own promoter at 4-cell responding to parental-to-zygotic transition, marks stemness, maintains expression...
Abstract In Arabidopsis , LTR-retrotransposons are activated by mutations in the chromatin remodeler DECREASE DNA METHYLATION 1 (DDM1), giving rise to 21-22nt epigenetically siRNAs (easiRNAs) that depend on RNA DEPENDENT POLYMERASE 6 (RDR6). We purified virus-like-particles (VLPs) from ddm1 and ddm1rdr6 mutants which genomic is reverse transcribed into complementary DNA. Next generation short-read long-read sequencing of VLP (VLP DNA-seq) revealed a comprehensive catalog active without need...