A5054804286- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Leptospirosis research and findings
- Chromosomal and Genetic Variations
- Viral Infections and Vectors
- Genomics and Chromatin Dynamics
- Calcium signaling and nucleotide metabolism
- CAR-T cell therapy research
- Advanced biosensing and bioanalysis techniques
- Cynara cardunculus studies
- Sphingolipid Metabolism and Signaling
- Research on Leishmaniasis Studies
- Erythrocyte Function and Pathophysiology
- Paraoxonase enzyme and polymorphisms
- Biochemical Acid Research Studies
- Molecular Biology Techniques and Applications
- Animal Genetics and Reproduction
Max Delbrück Center
2016-2023
University of Hyderabad
2011-2015
Pathogenic members of the genus Leptospira are causative agents leptospirosis, a neglected disease public and veterinary health concern. Leptospirosis is systemic that in its severest forms leads to renal insufficiency, hepatic dysfunction, pulmonary failure. Many strains produce hemolytic sphingomyelinase activities, number candidate leptospiral hemolysins have been identified based on sequence similarity well-characterized bacterial hemolysins. Five putative paralogs. Although recombinant...
<i>Background:</i> Leptospiral sphingomyelinases are candidate virulence factors present only in pathogenic <i>Leptospira</i> spp. <i>Leptospira interrogans</i> serovar Lai encodes Sph1, Sph2, Sph3, Sph4 and SphH. Except for Sph4, they all possess the exo-endo-phosphatase domain that groups them under DNase I superfamily. <i>Methods, Results Conclusions:</i> Modeling of domains reveals high-level structural similarity Sph2 with crystal...
Transposable elements are natural, non-viral gene delivery vehicles capable of mediating stable genomic integration. The Sleeping Beauty (SB) transposon has the ability to cut-and-paste 'gene interest' into genome, providing basis for long-term, permanent transgene expression in transgenic cells and organisms. SB system is relatively well characterized, been extensively engineered efficient discovery purposes a wide range vertebrates, including humans. safe simple-to-use vector that enables...
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Human serum paraoxonase1 (HuPON1) belongs to the family of A-esterases (EC.3.1.8.1).It is associated with HDL particle and prevents atherosclerosis by cleaving lipid hydroperoxides other proatherogenic molecules oxidized low density lipoproteins (LDL).Since precise structure HuPON1 not yet available, structure-function relationship between activators/inhibitors still unknown.Therefore, a theoretical model was generated using homology modelling molecular interactions an activator aspirin...
<h3>Background</h3> <i>Ex-vivo</i> modification of immune cells to express Chimeric Antigen Receptor (CAR) has shown tremendous clinical and commercial success as a cancer treatment. Despite its widespread adoption, <i>ex-vivo</i> CAR-T approaches face challenges such soaring production costs, extended timelines, inherent toxicity risks operational complexities. These limit the accessibility treatment highlight need for improved methods. Here, we demonstrate novel non-viral vector capable...
Transposons are parasitic genetic elements that frequently hijack vital cellular processes of their host. HMGXB4 is a known Wnt signaling-regulating HMG-box protein, previously identified as host-encoded factor Sleeping Beauty (SB) transposition. Here, we show predominantly maternally expressed, and marks both germinal progenitor somatic stem cells. SB piggybacks to activate transposase expression target transposition cells, thereby potentiating heritable transposon insertions. The promoter...
Abstract Transposons are parasitic genetic elements that frequently hijack key cellular processes of the host. HMGXB4 is a Wnt signalling-associated HMG-box protein, previously identified as transcriptional regulating host factor Sleeping Beauty (SB) transposition. Here, we establish highly expressed from zygote stage, and declines after genome activation. Nevertheless, activated by its own promoter at 4-cell responding to parental-to-zygotic transition, marks stemness, maintains expression...