A5067990337- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- CAR-T cell therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Research and Splicing
- Plant Virus Research Studies
Massachusetts Institute of Technology
2022
Cold Spring Harbor Laboratory
2017-2022
Abstract Precise, scalable, and sustainable control of genetic cellular activities in mammalian cells is key to developing precision therapeutics smart biomanufacturing. Here we create a highly tunable, modular, versatile CRISPR-based synthetic transcription system for the programmable gene expression phenotypes cells. Genetic circuits consisting well-characterized libraries guide RNAs, binding motifs operators, transcriptional activators, additional regulatory elements express genes...
Abstract RNA interference is required for post-transcriptional silencing, but also has additional roles in transcriptional silencing of centromeres and genome stability. However, these have been controversial mammals. Strikingly, we found that Dicer-deficient embryonic stem cells strong proliferation chromosome segregation defects as well increased transcription centromeric satellite repeats, which triggers the interferon response. We conducted a CRISPR-Cas9 genetic screen to restore...
Abstract Precise, scalable, and sustainable control of genetic cellular activities in mammalian cells is key to developing precision therapeutics smart biomanufacturing. We created a highly tunable, modular, versatile CRISPR-based synthetic transcription system for the programmable gene expression phenotypes cells. Genetic circuits consisting well-characterized libraries guide RNAs, binding motifs operators, transcriptional activators, additional regulatory elements expressed genes...