A5052263342- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Plant Virus Research Studies
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Plant Pathogens and Fungal Diseases
- Fungal Infections and Studies
- T-cell and B-cell Immunology
- Mosquito-borne diseases and control
- DNA Repair Mechanisms
- Viral Infections and Outbreaks Research
- Congenital Diaphragmatic Hernia Studies
- Light effects on plants
- Plant Molecular Biology Research
- Telomeres, Telomerase, and Senescence
- Viral Infections and Vectors
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Polyomavirus and related diseases
Cold Spring Harbor Laboratory
2016-2024
Howard Hughes Medical Institute
2016-2024
Gordon and Betty Moore Foundation
2016
Institut Pasteur
2008-2011
Centre National de la Recherche Scientifique
1997-2008
Université Paris-Sud
1997
Université Paris-Saclay
1997
RNAi soothes the path to quiescence Cells, such as those in stem cell niches and immunity memory cells, can exist a nondividing, quiescent state, from which they be aroused with appropriate signal. RNA interference (RNAi) is an important epigenetic pathway many organisms. Roche et al. found that fission yeast, was essential regulator of state. promoted proper chromosome segregation during entry into quiescence. It also prevented inappropriate silencing ribosomal DNA Science , this issue p....
Abstract Selfish genetic elements contribute to hybrid incompatibility and bias or ‘drive’ their own transmission 1,2 . Chromosomal drive typically functions in asymmetric female meiosis, whereas gene is normally post-meiotic found males. Here, using single-molecule single-pollen genome sequencing, we describe Teosinte Pollen Drive , an instance of hybrids between maize ( Zea mays ssp. ) teosinte mexicana Z. that depends on RNA interference (RNAi). 22-nucleotide small RNAs from a non-coding...
Background Most arthropod-borne viruses (arboviruses) are RNA viruses, which maintained in nature by replication cycles that alternate between arthropod and vertebrate hosts. Arboviruses appear to experience lower rates of evolution than replicate a single host. This genetic stability is assumed result from fitness trade-off imposed host alternation, constrains arbovirus genome evolution. To test this hypothesis, we used Rift Valley fever virus (RVFV), an can be transmitted either directly...
Abstract RNA interference is required for post-transcriptional silencing, but also has additional roles in transcriptional silencing of centromeres and genome stability. However, these have been controversial mammals. Strikingly, we found that Dicer-deficient embryonic stem cells strong proliferation chromosome segregation defects as well increased transcription centromeric satellite repeats, which triggers the interferon response. We conducted a CRISPR-Cas9 genetic screen to restore...
Meiotic drivers subvert Mendelian expectations by manipulating reproductive development to bias their own transmission. Chromosomal drive typically functions in asymmetric female meiosis, while gene is normally postmeiotic and found males. Using single molecule single-pollen genome sequencing, we describe
Transcriptional silencing by RNAi paradoxically relies on transcription, but how the transition from transcription to is achieved has remained unclear. The Cryptic Loci Regulator complex (CLRC) in Schizosaccharomyces pombe a cullin-ring E3 ligase required for that recruited RNAi. We found E2 ubiquitin conjugating enzyme Ubc4 interacts with CLRC and mono-ubiquitinates histone H3K9 methyltransferase Clr4
Somatic hypermutation (SHM) of Ig genes is the result a two-phase process initiated by activation-induced cytidine deaminase, relying on two different strategies for introduction mutations at CG pairs (phase I) and AT II). To explain selectivity phase II, mechanisms were proposed: AT-selective error-prone DNA-polymerases, deoxyuridine triphosphate (dUTP) incorporation, or both. However, there has been no experimental evidence so far possible involvement latter. We have developed...
Cryptococcus neoformans is responsible for life-threatening infections that primarily affect immunocompromised individuals and has an estimated worldwide burden of 220,000 new cases each year—with 180,000 resulting deaths—mostly in sub-Saharan Africa. Surprisingly, little known about the ecological niches occupied by C . nature. To expand our understanding distribution associations this pathogen we implement a Natural Language Processing approach to better describe niche We use Latent...
Somatic hypermutation (SHM) of immunoglobulin genes is currently viewed as a two step process initiated by the deamination deoxycytidine (C) to deoxyuridine (U), catalysed activation induced deaminase (AID). Phase 1 mutations arise from DNA replication across uracil residue or abasic site, generated uracil-DNA glycosylase, yielding transitions transversions at G:C pairs. 2 result recognition U∶G mismatch Msh2/Msh6 complex (MutS Homologue), followed excision mismatched nucleotide and repair,...
The nucleotide sequence of 17.3 kbp downstream addA (98°) on the Bacillus subtilis chromosome was determined. Twenty putative ORFs were identified. Three them coincided with known B. genes, addA, sbcD and wprA. product four other showed similarity to SbcC Clostridium perfringens , CotH 2-hydroxyhepta-2,4-diene-1,7-diodate isomerase Methanococcus jannaschi a ORF Pseudomonas syringae. In addition, 7.6 citG (189°) analysed. Among 10 identified, two mrgA whilst three homology X86780, sensory...
The approximately 10 kbp region encompassing nprB and argJ at 102 degrees on the Bacillus subtilis chromosome was sequenced, revealing 12 ORFs, four known genes (argJ, argC, ipi nprB) two genes, yitY yitS, whose products respectively display significant homology with L-gulono-gamma-lactone oxidase of rat dihydrofolate reductase Staphylococcus aureus. data also indicated that mapped to a different position than previously published.
The 25·9 kbp region upstream of nprB at 100°-102° on the Bacillus subtilis chromosome was sequenced. This revealed a known gene, degA , which previously mislocated genetic map. A total 29 putative ORFs were identified including cluster three whose products show clear homology with sulphate adenylyl pathway enzymes and, in addition, 11 have one or more membrane domains, as indicated by their hydropathy profiles.
Abstract Transcriptional silencing by RNAi paradoxically relies on transcription, but how the transition from transcription to is achieved has remained unclear. The Cryptic Loci Regulator complex (CLRC) in Schizosaccharomyces pombe a cullin-ring E3 ligase required for that recruited RNAi. We found E2 ubiquitin conjugating enzyme Ubc4 interacts with CLRC and mono-ubiquitinates histone H3K9 methyltransferase Clr4 SUV39H1 , promoting co-transcriptional gene (H3K9me2) transcriptional (H3K9me3)....
Abstract Cryptococcus neoformans is responsible for life-threatening infections that primarily affect immunocompromised individuals and has an estimated worldwide burden of 220,000 new cases each year—with 180,000 resulting deaths—mostly in sub-Saharan Africa. Surprisingly, little known about the ecological niches occupied by C. nature. To expand our understanding distribution associations this pathogen we implement a Natural Language Processing approach to better describe niche . We use...
Abstract RNA interference is essential for transcriptional silencing and genome stability, but conservation of this role in mammals has been difficult to demonstrate. Dicer1 -/- mouse embryonic stem cells have microRNA-independent proliferation defects, we conducted a CRISPR-Cas9 screen restore viability. We identified suppressor mutations activators, H3K9 methyltransferases, chromosome segregation factors, strongly resembling Dicer suppressors fission yeast. Suppressors rescued chromosomal...