A5103093640- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- BRCA gene mutations in cancer
- DNA Repair Mechanisms
- Virus-based gene therapy research
- Ubiquitin and proteasome pathways
- Glioma Diagnosis and Treatment
Howard Hughes Medical Institute
2019-2023
Cold Spring Harbor Laboratory
2018-2023
Nucleosomes block access to DNA methyltransferase, unless they are remodeled by DECREASE in METHYLATION 1 (DDM1LSH/HELLS), a Snf2-like master regulator of epigenetic inheritance. We show that DDM1 promotes replacement histone variant H3.3 H3.1. In ddm1 mutants, methylation is partly restored loss the chaperone HIRA, while H3.1 CAF-1 becomes essential. The single-particle cryo-EM structure at 3.2 Å with nucleosome reveals engagement near residues required for assembly and unmodified H4 tail....
Epigenetic inheritance refers to the faithful replication of DNA methylation and histone modification independent sequence. Nucleosomes block access methyltransferases, unless they are remodeled by DECREASE IN METHYLATION1 (DDM1
Abstract Transcriptional silencing by RNAi paradoxically relies on transcription, but how the transition from transcription to is achieved has remained unclear. The Cryptic Loci Regulator complex (CLRC) in Schizosaccharomyces pombe a cullin-ring E3 ligase required for that recruited RNAi. We found E2 ubiquitin conjugating enzyme Ubc4 interacts with CLRC and mono-ubiquitinates histone H3K9 methyltransferase Clr4 SUV39H1 , promoting co-transcriptional gene (H3K9me2) transcriptional (H3K9me3)....