A5040578598- Microbial Community Ecology and Physiology
- Methane Hydrates and Related Phenomena
- Genomics and Phylogenetic Studies
- Marine and coastal ecosystems
- Peptidase Inhibition and Analysis
- Isotope Analysis in Ecology
- Marine Biology and Ecology Research
- Machine Learning in Bioinformatics
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Food Industry and Aquatic Biology
- Ocean Acidification Effects and Responses
- Polar Research and Ecology
- Protein Hydrolysis and Bioactive Peptides
- Bone and Dental Protein Studies
- Environmental DNA in Biodiversity Studies
- Advanced Proteomics Techniques and Applications
- Marine Bivalve and Aquaculture Studies
- Gene expression and cancer classification
- Atmospheric and Environmental Gas Dynamics
- Hydrocarbon exploration and reservoir analysis
- Enzyme Production and Characterization
- Nuclear Physics and Applications
- Indigenous Studies and Ecology
- Wastewater Treatment and Nitrogen Removal
University of Tennessee at Knoxville
2015-2024
University of Southern California
2024
University of South Alabama
2023
Max Planck Institute for Chemistry
2023
Dauphin Island Sea Lab
2023
Lawrence Berkeley National Laboratory
2023
Oregon State University
2023
Marine Biological Laboratory
2023
Florida State University
2023
University of Stirling
2021
To describe a microbe's physiology, including its metabolism, environmental roles, and growth characteristics, it must be grown in laboratory culture. Unfortunately, many phylogenetically novel groups have never been cultured, so their physiologies only inferred from genomics characteristics. Although the diversity, or number of different taxonomic groups, uncultured clades has studied well, global abundances, numbers cells any given environment, not assessed. We quantified degree similarity...
ABSTRACT In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys water column sediments. an fjord (Smeerenburgfjord) Svalbard, , together with Flavobacteria smaller proportions Alpha - Gammaproteobacteria constituted most frequently detected bacterioplankton community gene-based library analyses column. Parallel measurements activities six endo-acting...
This study offers insight into the roles anodic and cathodic processes play in electrochemically activated persulfate (EAP) screens EAP as a viable technique for ciprofloxacin degradation wastewater. Sulfate radical formation at boron-doped diamond (BDD) anode activation graphite cathode were experimentally elucidated using different electrolytes electrochemical setups. Rapid transformation occurred via pseudo-first-order mechanisms with respect to electrolyte, reaching 84% removal 120 min...
There is no universally accepted method to quantify bacteria and archaea in seawater marine sediments, different methods have produced conflicting results with the same samples. To identify best practices, we compiled data from 65 studies, plus our own measurements, which were quantified fluorescent situ hybridization (FISH), catalyzed reporter deposition FISH (CARD-FISH), polyribonucleotide FISH, or quantitative PCR (qPCR). estimate efficiency, defined "yield" be sum of counted by these...
Heterotrophic microbial communities cycle nearly half of net primary productivity in the ocean, and play a particularly important role transformations dissolved organic carbon (DOC). The specific means by which these mediate are largely unknown, since vast majority marine bacteria have not been isolated culture, most measurements DOC degradation rates focused on uptake metabolism either bulk or simple model compounds (e.g. amino acids sugars). Genomic investigations provide information about...
Much of life on Earth exists in a very slow-growing state, with microbes from deeply buried marine sediments representing an extreme example. These environments are like natural laboratories that have run multi-thousand-year experiments impossible to perform laboratory. We borrowed some techniques commonly used laboratory and applied them these samples make hypotheses about how subsist for so long at low activity. found methods stabilizing proteins nucleic acids might be by many members the...
Summary Aquatic sediments harbour diverse microbial communities that mediate organic matter degradation and influence biogeochemical cycles. The pool of bioavailable carbon continuously changes as a result abiotic processes activity. It remains unclear how respond to heterogeneous matrices this ultimately affects heterotrophic respiration. To explore the relationships between mixed substrates activity, we incubated batches organic‐rich in novel bioreactor (IsoCaRB) permitted continuous...
Advances in sampling tools, analytical methods and data handling capabilities have been fundamental to the growth of marine organic biogeochemistry over past four decades. There has always a strong feedback between advances scientific advances. However, whereas technology were often driving force that made possible progress elucidating sources fate matter ocean first decades biogeochemistry, today process-based questions should drive developments. are several paradigm shifts challenges for...
Abstract. Marine snow aggregates are heavily colonized by heterotrophic microorganisms that express high levels of hydrolytic activities, making hotspots for carbon remineralization in the ocean. To assess how aggregate formation influences ability seawater microbial communities to access organic carbon, we compared hydrolysis rates six polysaccharides coastal after had been formed (via incubation on a roller table) with from same site not incubated table (referred as whole seawater)....
The 'priming effect', in which addition of labile substances changes the remineralization rate recalcitrant organic matter, has been intensively studied soils, but is less well-documented aquatic systems. We investigated extent to additions nutrients or carbon could influence rates 14C-labeled, microbially-degraded, phytoplankton-derived matter (OM) microcosms inoculated with microbial communities drawn from Grove's Creek Estuary coastal Georgia, USA. found that amendment protein plus...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 75:271-281 (2015) - DOI: https://doi.org/10.3354/ame01755 Substrate specificity of aquatic extracellular peptidases assessed by competitive inhibition assays using synthetic substrates Andrew D. Steen1,*, Jasmine P. Vazin2, Shane M. Hagen3, Katherine H. Mulligan2,4, Steven W. Wilhelm2 1Department Earth and Planetary...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 69:93-99 (2013) - DOI: https://doi.org/10.3354/ame01625 Extracellular peptidase and carbohydrate hydrolase activities in an Arctic fjord (Smeerenburgfjord, Svalbard) Andrew D. Steen1,3,*, Carol Arnosti2 1Center for Geomicrobiology, Aarhus University, 8000 C, Denmark 2Department of Marine Sciences, University North...
The ratios of d- versus l-amino acids can be used to infer the sources and composition sedimentary organic matter. Such inferences, however, rely on knowing rates at which amino in matter racemize abiotically between l-forms. Based a heating experiment, we report kinetic parameters for racemization aspartic acid, glutamic serine, alanine bulk sediment from Aarhus Bay, Denmark, taken surface, 30 cm, 340 cm depth below seafloor. Extrapolation typical cold deep sea temperature 3°C suggests rate...
Burial of organic carbon in marine and estuarine sediments represents a long-term sink for atmospheric dioxide. Globally, ∼40% burial occurs anoxic estuaries deltaic systems. However, the ultimate controls on amount matter that is buried sediments, versus oxidized into CO 2 , are poorly constrained. In this study, we used combination enzyme assays metagenomic analysis to identify how subsurface microbial communities catalyze first step proteinaceous degradation. Our results show deeper...
Microbial communities in terrestrial geothermal systems often contain chemolithoautotrophs with well-characterized distributions and metabolic capabilities. However, the extent to which organic matter produced by these supports heterotrophs remains largely unknown. Here we compared abundance activity of peptidases carbohydrate active enzymes (CAZymes) that are predicted be extracellular identified metagenomic assemblies from 63 springs Central American Andean convergent margin (Argentinian...