A5035081697- Methane Hydrates and Related Phenomena
- Geophysics and Gravity Measurements
- Offshore Engineering and Technologies
- Microbial Community Ecology and Physiology
- Astronomical Observations and Instrumentation
- Hydrocarbon exploration and reservoir analysis
- Geomagnetism and Paleomagnetism Studies
- Geological and Geophysical Studies
- Spacecraft and Cryogenic Technologies
- Solar and Space Plasma Dynamics
- Atmospheric and Environmental Gas Dynamics
- Planetary Science and Exploration
- Geology and Paleoclimatology Research
- Scientific Research and Discoveries
- Astro and Planetary Science
- Geological Modeling and Analysis
- Genomics and Phylogenetic Studies
- Inertial Sensor and Navigation
- Drilling and Well Engineering
- Bacteriophages and microbial interactions
- Paleontology and Stratigraphy of Fossils
- Gut microbiota and health
- Environmental Monitoring and Data Management
- Protist diversity and phylogeny
- Nuclear Physics and Applications
Walter Reed Army Institute of Research
2022-2024
University of Tennessee at Knoxville
2013-2024
Colorado State University
2024
International Ocean Discovery Program
2018
There is no universally accepted method to quantify bacteria and archaea in seawater marine sediments, different methods have produced conflicting results with the same samples. To identify best practices, we compiled data from 65 studies, plus our own measurements, which were quantified fluorescent situ hybridization (FISH), catalyzed reporter deposition FISH (CARD-FISH), polyribonucleotide FISH, or quantitative PCR (qPCR). estimate efficiency, defined "yield" be sum of counted by these...
The subduction of seamounts and ridge features at convergent plate boundaries plays an important role in the deformation overriding influences geochemical cycling associated biological processes. Active serpentinization forearc mantle serpentinite mud volcanism on Mariana (between trench active volcanic arc) provides windows Here, we present (1) first observation extensive exposure undeformed Cretaceous seamount currently being subducted Trench inner slope; (2) vertical region related to...
Streptococcus pneumoniae remains a leading cause of bacterial pneumonia and meningitis in children globally, despite pneumococcal conjugate vaccine (PCV) implementation. We report case fatal fully vaccinated 2-year-old male. Despite prior PCV13 vaccination, the patient developed caused by S. serotype 15B cerebrospinal fluid 15C blood. Genomic sequencing revealed nearly identical isolates differing capsular switch mutation. This highlights limitations against emerging serotypes like 15C. The...
Burial of organic carbon in marine and estuarine sediments represents a long-term sink for atmospheric dioxide. Globally, ∼40% burial occurs anoxic estuaries deltaic systems. However, the ultimate controls on amount matter that is buried sediments, versus oxidized into CO 2 , are poorly constrained. In this study, we used combination enzyme assays metagenomic analysis to identify how subsurface microbial communities catalyze first step proteinaceous degradation. Our results show deeper...
The difficulty involved in quantifying biogeochemically significant microbes marine sediments limits our ability to assess interspecific interactions, population turnover times, and niches of uncultured taxa. We incubated surface from Cape Lookout Bight, North Carolina, USA, anoxically at 21°C for 122 days. Sulfate decreased until day 68, after which methane increased, with hydrogen concentrations consistent the predicted values an electron donor exerting thermodynamic control. measured...
We describe the genome of a lytic phage EAb13 isolated from sewage, with broad activity against multidrug-resistant Acinetobacter baumannii. is an unclassified siphovirus. Its consists 82,411 bp, 40.15% GC content, 126 protein-coding sequences, 1 tRNA, and 2,177 bp-long direct terminal repeats.
As marine sediments are buried, microbial communities transition from sulfate-reduction to methane-production after sulfate is depleted. When this biogenic methane diffuses into the overlying sulfate-rich sediments, it forms a sulfate-methane zone (SMTZ) because reducers deplete hydrogen concentrations and make hydrogenotrophic methanogenesis exergonic in reverse direction, process called anaerobic oxidation of (AOM). Microbial participation these processes often inferred geochemistry,...
Global marine sediments harbor a large and highly diverse microbial biosphere, but the mechanism by which this biosphere is established during sediment burial largely unknown. During in sediments, concentrations of easily metabolized organic compounds total cell abundance decrease. However, it unknown whether some clades increase with depth. We show population increases 38 families over 3 cm depth upper 7.5 White Oak River (WOR) estuary sediments. Clades that increased were more often...
Shigellosis is a leading global cause of diarrheal disease and travelers’ diarrhea now being complicated by the dissemination antibiotic resistance, necessitating development alternative antibacterials such as therapeutic bacteriophages (phages). Phages with lytic activity against Shigella strains were isolated from sewage. The genomes 32 phages sequenced, based on genomic comparisons belong to seven taxonomic genera: Teetrevirus, Teseptimavirus, Kayfunavirus, Tequatrovirus, Mooglevirus,...
Marine deep subsurface sediment is often a microbial environment under energy-limited conditions. However, life has been found to persist and even thrive in environments. The Mariana forearc represents an ideal location for determining how can withstand extreme conditions including pH 10-12.5 depleted nutrients. International Ocean Discovery Program Expedition 366 the Convergent Margin sampled three serpentinizing seamounts located along chain with elevated concentrations of methane,...
Abstract Uncultured members of the Methanomicrobia called ANME-1 perform anaerobic oxidation methane (AOM) through a process that uses much methanogenic pathway. It is unknown whether obligately AOM, or some them can methanogenesis when exergonic. Most marine sediments lack advective transport methane, so AOM occurs in sulfate transition zone (SMTZ) where sulfate-reducing bacteria consume hydrogen produced by fermenters, making hydrogenotrophic exergonic reverse direction. When depleted...
Abstract Global marine sediments harbor a large and highly diverse microbial biosphere, but the mechanism by which this biosphere is established during sediment burial largely unknown. During in sediments, concentrations of easily-metabolized organic compounds total cell abundance decrease steadily. However, it unknown whether some clades increase with depth, despite overall trend decrease. We show population increases 38 families over 3 cm depth upper 7.5 White Oak River (WOR) estuary...
With a rise in antibiotic resistance and chronic infection, the metabolic response of
ABSTRACT With a rise in antibiotic resistance and chronic infection, the metabolic response of Salmonella enterica serovar Typhimurium to various dietary conditions over time remains an understudied avenue for novel, targeted therapeutics. Elucidating how enteric pathogens respond variation not only helps us decipher strategies leveraged expansion but also assists proposing targets therapeutic interventions. In this study, we use multi-omics approach identify mice on both fibrous diet...
Molecular hydrogen is produced by the fermentation of organic matter and consumed organisms including hydrogenotrophic methanogens sulfate reducers in anoxic marine sediment. The thermodynamic feasibility these metabolisms depends strongly on reactivity concentrations; low high concentrations can inhibit so when poor, fermenters might form syntrophies with and/or who alleviate stress keeping tightly controlled. However, it unclear how effect porewater natural sediments different reactivities.