A5105932559- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Antibiotics Pharmacokinetics and Efficacy
- Tuberculosis Research and Epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- Bacterial Identification and Susceptibility Testing
- Mycobacterium research and diagnosis
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Travel-related health issues
- Parasites and Host Interactions
- Molecular Biology Techniques and Applications
- Eosinophilic Esophagitis
- Pediatric health and respiratory diseases
- Animal Disease Management and Epidemiology
- Berberine and alkaloids research
- Disaster Response and Management
- Pharmaceutical and Antibiotic Environmental Impacts
- Poisoning and overdose treatments
United States Army Medical Research Institute of Infectious Diseases
2025
Walter Reed Army Institute of Research
2013-2024
Uniformed Services University of the Health Sciences
2021
Armed Forces Research Institute of Medical Science
2017-2020
East Avenue Medical Center
2017
United States Army Medical Research Directorate - Africa
2013
ESKAPEE pathogens Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. and Escherichia coli are multi-drug resistant (MDR) bacteria that present increasing treatment challenges for healthcare institutions public health worldwide.431 MDR were collected from Queen Sirikit Naval Hospital, Chonburi, Thailand between 2017 2018. Species identification antimicrobial resistance (AMR) phenotype determined following CLSI...
Hospitalized patients are at risk of developing serious multidrug resistant bacterial infections. This is heightened in who on mechanical ventilation, immunocompromised, and/or have chronic comorbidities. We report the case a 52-year-old critically ill patient with Acinetobacter baumannii (MDR-A) respiratory infection was successfully treated antibiotics and intravenous nebulized bacteriophage therapy.
Abstract Objectives Pseudomonas aeruginosa is a leading cause of community- and hospital-acquired infections. Successful treatment hampered by its remarkable ability to rapidly develop resistance antimicrobial agents, primarily through mutation. In response, WHO listed carbapenem-resistant P. as Priority 1 (Critical) pathogen for research development new treatments. A key resource in developing effective countermeasures access diverse clinically relevant strains testing. Herein we describe...
Staphylococcus aureus (S. aureus), especially methicillin-resistant S. (MRSA), remains a major public health concern. This study reports the antimicrobial resistance profiles and molecular characteristics of 31 isolated during 2017–2018 from inpatient outpatient clinical specimens Queen Sirikit Naval Hospital (QSH) in Chonburi province, Thailand. All isolates were tested for susceptibility. Staphylococcal cassette chromosome mec (SCCmec) typing, Panton–Valentine leukocidin (pvl) toxin,...
Recently there have been calls for the eradication of malaria and elimination soil-transmitted helminths (STHs). Malaria STHs overlap in distribution, STH infections are associated with increased risk malaria. Indeed, is evidence that suggests infection may facilitate transmission. coinfection exacerbate anemia, especially pregnant women, leading to worsened child development more adverse pregnancy outcomes than these diseases would cause on their own. Ivermectin mass drug administration...
Providencia rettgeri is an emerging opportunistic Gram-negative pathogen with reports of increasing antibiotic resistance. Pan-drug resistant (PDR) P. infections are a growing concern, demonstrating need for the development alternative treatment options which fueling renewed interest in bacteriophage (phage) therapy. Here, we identify and characterize phage vB_PreP_EPr2 (EPr2) lytic activity against PDR MRSN 845308, clinical isolate that carries multiple resistance genes. EPr2 was isolated...
Antibiotic resistance, when it comes to bacterial infections, is not a problem that going disappear anytime soon. With the lack of larger investment in novel antibiotic research and ever-growing increase resistant isolates amongst ESKAPEE pathogens (Enterobacter cloacae, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterococcus sp., Escherichia coli), inevitable more infections caused by extensively drug-resistant (XDR) pandrug-resistant...
During 2013-2016, we isolated blaNDM- and blaVIM-harboring Enterobacteriaceae nonfermentative bacteria from patients in the Philippines. Of 130 carbapenem-resistant isolates tested, 45 were Carba NP-positive; 43 harbored blaNDM, 2 blaVIM. Multidrug-resistant microbial pathogen surveillance antimicrobial drug stewardship are needed to prevent further spread of New Delhi metallo-β-lactamase variants.
Shigellosis is a leading global cause of diarrheal disease and travelers’ diarrhea now being complicated by the dissemination antibiotic resistance, necessitating development alternative antibacterials such as therapeutic bacteriophages (phages). Phages with lytic activity against Shigella strains were isolated from sewage. The genomes 32 phages sequenced, based on genomic comparisons belong to seven taxonomic genera: Teetrevirus, Teseptimavirus, Kayfunavirus, Tequatrovirus, Mooglevirus,...
Abstract Adjuvant therapy with bacteriophage (phage) cocktails in combination antibiotics is a therapeutic approach currently considered for treatment of infections encapsulated, biofilm forming, and multidrug-resistant Klebsiella pneumoniae (Kp). phage are highly selective targeting bacterial capsule type. Considering the numerous Kp types other host restriction factors, could be facilitated when generating phages broad range A modified ‘Appelmans protocol’ was used to create an extended...
Diarrhea is a serious concern worldwide, especially in developing countries. Rotavirus implicated approximately 400,000 infant deaths annually. It highly contagious elevating the risk of outbreaks enclosed settings such as daycare centers, hospitals, and boarding schools. Reliable testing methods are critical for early detection infections, better clinical management, pathogen surveillance evaluation interventions vaccines. Enzyme immunoassays have proved to be reliable practical most...
The first report of a plasmid-borne colistin resistance gene (mcr-1) detected in an Escherichia coli isolate from China heralded the emergence pandrug-resistant bacteria. Since then, mcr-1 has been worldwide, but to date it not reported Philippines. In this study, 123 antimicrobial-resistant isolates collected January-June 2018 patients admitted tertiary hospital Manila, Philippines, were characterised. Biochemical identification and antimicrobial susceptibility testing performed using BD...
Here, we describe genome sequences of 17 Pseudomonas aeruginosa phages, including therapeutic candidates. They belong to the families Myoviridae , Podoviridae and Siphoviridae six different genera. The genomes ranged in size from 42,788 88,805 bp, with G+C contents 52.5% 64.3%, numbers coding 58 179.
Abstract A severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) B.1.1.345 variant carrying the E484K mutation was detected in 4 patients with no apparent epidemiological association from a hospital network upstate New York. Subsequent analysis identified an additional 11 variants this region between December 2020 and February 2021.
Travelers' diarrhea (TD) is common among military personnel deployed to tropical and subtropical regions. It remains unclear how TD subsequent antibiotic treatment impact the resident microflora within gut, especially given increased prevalence of resistance enteric pathogens acquisition multidrug-resistant organisms. We examined functional properties fecal in response TD, along with treatment. Fecal samples from US UK service members Djibouti, Kenya, Honduras who presented acute watery were...
Cefazolin is a first-line antibiotic to treat infection related deployment-associated blast injuries. Prior literature demonstrated 331% increase cefazolin liver area under the curve (AUC) in mice exposed survivable compared with controls. We repeated experiment, validated findings, and established semimechanistic two-compartment pharmacokinetic (PK) model effect compartments representing skin. found that statistically significantly increased pseudo–partition coefficient by 326% (95%...
Abstract Ongoing surveillance detected a SARS-CoV-2 B.1.1.220 variant carrying the E484K substitution in four patients from hospital network upstate New York. Patients reported no travel history and shared obvious epidemiological linkage. A search of online databases identified 12 additional with E484K, all which were York since December 2020. Detailed genomic analyses suggests that mutation has emerged independently at least two different strains this region.
Abstract ESKAPEE pathogens ( Enterococcus spp., Staphylococcus aureus , Klebsiella pneumoniae Acinetobacter baumannii Pseudomonas aeruginosa Enterobacter and Escherichia coli ), particularly the gram-negative species, are a problematic group of bacteria associated with nosocomial infections in susceptible patients. These often further complicated by multidrug-resistant (MDR) strains lack treatment options. While pipeline new antibiotics is nothing short thin when it comes to activity, one...
Abstract Objective Stool repositories are a valuable resource for retrospective analyses including quantitative PCR assays to distinguish between asymptomatic shedding and clinical disease. The suitability of archival specimens this purpose is unclear requires assessment. We conducted pilot study evaluate pathogen detection by TaqMan Array Card (TAC) in travelers’ diarrhea (TD) stool stored 1–13 years, as well as, the impact transporting on Whatman FTA Elute cards (FTA Cards) detection....
Abstract Pseudomonas aeruginosa is a leading cause of community-acquired and hospital-acquired infections. Successful treatment hampered by its remarkable ability to rapidly develop resistance antimicrobial agents, mostly through mutation. In response, the World Health Organization listed carbapenem-resistant P. as Priority 1 (Critical) pathogen for research development new treatments. A key resource in developing effective countermeasures access diverse clinically relevant strains testing....