A5076969183- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Genomics and Phylogenetic Studies
- Yersinia bacterium, plague, ectoparasites research
- Bacillus and Francisella bacterial research
- Plant Virus Research Studies
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Burkholderia infections and melioidosis
- Vibrio bacteria research studies
- Monoclonal and Polyclonal Antibodies Research
- Bacterial Identification and Susceptibility Testing
- Brucella: diagnosis, epidemiology, treatment
- Vector-borne infectious diseases
- Plant-based Medicinal Research
- Heat shock proteins research
- Pharmacological Effects of Natural Compounds
- Infections and bacterial resistance
- Viral gastroenteritis research and epidemiology
- Antimicrobial Resistance in Staphylococcus
- Lipid Membrane Structure and Behavior
- Plant and Fungal Interactions Research
- Escherichia coli research studies
- Bacterial biofilms and quorum sensing
- Antimicrobial Peptides and Activities
Walter Reed Army Institute of Research
2014-2025
Russian Scientific Research Institute Microbe
1995-2007
Bacteriophages specific for Yersinia pestis are routinely used plague diagnostics and could be an alternative to antibiotics in case of drug-resistant plague. A major concern bacteriophage therapy is the emergence phage-resistant mutants. The use phage cocktails can overcome this problem but only if phages exploit different receptors. Some mutants lose virulence therefore should not complicate therapy.The purpose work was identify Y. receptors using site-directed mutagenesis...
Multidrug-resistant (MDR) Pseudomonas aeruginosa infections pose a serious health threat. Bacteriophage–antibiotic combination therapy is promising candidate for combating these infections. A 5-phage P. cocktail, PAM2H, was tested in with antibiotics (ceftazidime, ciprofloxacin, gentamicin, meropenem) to determine if PAM2H enhances antibiotic activity. Combination treatment vitro resulted significant increase susceptibility of MDR strains antibiotics. Treatment ceftazidime (CAZ), meropenem,...
Abstract Background Antimicrobial resistance (AMR) is undermining modern medicine, a problem compounded by bacterial adaptation to antibiotic pressures. Phages are viruses that infect bacteria. Their diversity and evolvability offer the prospect of their use as therapeutic solution. Reported outcomes customized phage therapy for patients with difficult-to-treat antimicrobial resistant infections. Methods We retrospectively assessed 12 cases from production center. were screened, purified,...
ABSTRACT Left ventricular assist devices (LVAD) are increasingly used for management of heart failure; infection remains a frequent complication. Phage therapy has been successful in variety antibiotic refractory infections and is interest treating LVAD infections. We performed retrospective review four patients that underwent five separate courses intravenous (IV) phage with concomitant treatment endovascular Pseudomonas aeruginosa infection. assessed susceptibility, bacterial strain...
Abstract Background Bacteriophages (phages) are a promising anti-infective option for human disease. Major gaps remain in understanding their potential utility. Methods This is randomized, placebo-controlled, double-blind study of single dose intravenous phage approximately 72 clinically stable adult cystic fibrosis volunteers recruited from up to 20 US sites with Pseudomonas aeruginosa airway colonization. The consists mixture four anti-pseudomonal phages. Six sentinel participants will be...
Staphylococci are frequent agents of health care-associated infections and include methicillin-resistant Staphylococcus aureus (MRSA), which is resistant to first-line antibiotic treatments. Bacteriophage (phage) therapy a promising alternative antibacterial option treat MRSA infections. S. aureus-specific phage Sb-1 has been widely used in Georgia variety human broad host range within aureus, including strains, its can be further expanded by adaptation previously clinical isolates. The...
Background Yersinia pestis, the agent of plague, has caused many millions human deaths and still poses a serious threat to global public health. Timely reliable detection such dangerous pathogen is critical importance. Lysis by specific bacteriophages remains an essential method Y. pestis plague diagnostics. Methodology/Principal Findings The objective this work was develop alternative conventional phage lysis tests – rapid highly sensitive indirect live cells based on quantitative real-time...
Phage therapeutics offer a potentially powerful approach for combating multidrug-resistant bacterial infections. However, to be effective, phage therapy must overcome existing and developing resistance. While cocktails can reduce this risk by targeting multiple receptors in single therapeutic, bacteria have mechanisms of resistance beyond receptor modification. A rapidly growing body knowledge describes broad varied arsenal antiphage systems encoded counter infection. We sought understand...
ABSTRACT We describe the genomes of three lytic Pseudomonas aeruginosa phages genus Phikmvvirus . The vB_Pae4841-AFR43, vB_Pae10145-KEN1, and vB_Pae9718-KEN10 consist 43,426, 43,406, 43,118 bp, with 62.4%, 62.3%, 62.2% GC content, contain 63, 66, 64 coding sequences, respectively, no tRNA genes.
ABSTRACT We describe the genomes of two Pseudomonas aeruginosa phages genus Bruynoghevirus , WRAIR_EPa83 and WRAIR_EPa87. They consist 45,622 45,077 bp, with 52.52% 52.11% guanine-cytosine content, contain 81 80 coding sequences, three tRNA genes, direct terminal repeats 183 184 respectively.
ABSTRACT We describe the genome of a lytic phage EKq1 isolated on Klebsiella quasipneumoniae , with activity against pneumoniae . is an unclassified representative class Caudoviricetes, similar to phages VLCpiS8c, phiKp_7-2, and vB_KleS-HSE3. The 48,244-bp has GC content 56.43% 63 predicted protein-coding genes.
For decades, bacteriophages (phages) have been used for Brucella species identification in the diagnosis and epidemiology of brucellosis. Traditional phage typing is a multi-day procedure including isolation pure culture, step that can take up to three weeks. In this study, we focused on use brucellaphages sensitive detection pathogen clinical other complex samples, developed an indirect method using real-time quantitative PCR monitoring brucellaphage DNA amplification via replication live...
A potential concern with bacteriophage (phage) therapeutics is a host-versus-phage response in which the immune system may neutralize or destroy phage particles and thus impair therapeutic efficacy, strong inflammatory to repeated exposure might endanger patient. Current literature discrepant regard nature magnitude of innate adaptive phages. The purpose this work was study effects Staphylococcus aureus K on activation human monocyte-derived dendritic cells. Since acquired from ATCC isolated...
The spread of natural or weaponized drug-resistant plague among humans is a credible high consequence threat to public health that demands the prompt introduction alternatives antibiotics such as bacteriophage. Early attempts treat with phages in 1920s-1930s were sometimes promising but mostly failed, purportedly due insufficient knowledge phage biology and poor experimental design. We recently reported striking stability diagnostic bacteriophages, their safety for animal use, propagation...
Providencia rettgeri is an emerging opportunistic Gram-negative pathogen with reports of increasing antibiotic resistance. Pan-drug resistant (PDR) P. infections are a growing concern, demonstrating need for the development alternative treatment options which fueling renewed interest in bacteriophage (phage) therapy. Here, we identify and characterize phage vB_PreP_EPr2 (EPr2) lytic activity against PDR MRSN 845308, clinical isolate that carries multiple resistance genes. EPr2 was isolated...
We describe the genome of a lytic phage EAb13 isolated from sewage, with broad activity against multidrug-resistant Acinetobacter baumannii. is an unclassified siphovirus. Its consists 82,411 bp, 40.15% GC content, 126 protein-coding sequences, 1 tRNA, and 2,177 bp-long direct terminal repeats.
Standardized approaches to phage susceptibility testing (PST) are essential inform selection of phages for study in patients with bacterial infections. There is no reference standard assessing phage. We compared agreement between PST performed at three centers: two centers using a liquid assay standardized the sites third, plaque assay. Four
Plague, anthrax and brucellosis are severe bacterial infections presenting a serious threat to public health. Their causative agents can be weaponized number of drug-resistant strains have been described. This requires improvement existing development new methods diagnostics, strain characterization, prophylaxis therapy these infections. review article focuses on lytic bacteriophages (phages) active against Yersinia pestis, Bacillus anthracis Brucella including the uses phages for typing,...