A5053041466- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- Viral gastroenteritis research and epidemiology
- Bacterial Identification and Susceptibility Testing
- Respiratory viral infections research
- Bacillus and Francisella bacterial research
- Vibrio bacteria research studies
- Infections and bacterial resistance
- RNA and protein synthesis mechanisms
- Viral Infections and Outbreaks Research
- Antimicrobial Peptides and Activities
- Viral Infections and Vectors
- Clostridium difficile and Clostridium perfringens research
- Genetics, Bioinformatics, and Biomedical Research
- Orthopedic Infections and Treatments
- Innovation Policy and R&D
- Infective Endocarditis Diagnosis and Management
- Lipid Membrane Structure and Behavior
- Nuclear reactor physics and engineering
- Surgical site infection prevention
- Graphite, nuclear technology, radiation studies
Naval Medical Research Command
2016-2024
Nuffield Orthopaedic Centre
2021-2023
University of Oxford
2023
Naval Hospital Jacksonville
2021-2022
Theodore Roosevelt High School
2021-2022
ABSTRACT Widespread antibiotic use in clinical medicine and the livestock industry has contributed to global spread of multidrug-resistant (MDR) bacterial pathogens, including Acinetobacter baumannii . We report on a method used produce personalized bacteriophage-based therapeutic treatment for 68-year-old diabetic patient with necrotizing pancreatitis complicated by an MDR A. infection. Despite multiple courses efforts at percutaneous drainage pancreatic pseudocyst, deteriorated over...
Bacteriophage therapy (BT) uses bacteriophages to treat pathogenic bacteria and is an emerging strategy against multidrug-resistant (MDR) infections. Experience in solid organ transplant limited. We describe BT 3 lung recipients (LTR) with life-threatening MDR infections caused by Pseudomonas aeruginosa (n = 2) Burkholderia dolosa 1). For each patient, lytic were selected their bacterial isolates. was administered for variable durations under emergency Investigational New Drug applications...
In the era of antibiotic resistance, alternative treatment options for multidrug-resistant bacterial infections are being explored. We present a case Acinetobacter baumannii infection treated with bacteriophages. Clinical trials needed to further investigate bacteriophage therapy as an option treat infections.
Multi-drug resistance is increasing at alarming rates. The efficacy of phage therapy, treating bacterial infections with bacteriophages alone or in combination traditional antibiotics, has been demonstrated emergency cases the United States and other countries, however remains to be approved for wide-spread use US. One limiting factor a lack guidelines assessing genomic safety candidates. We present characterization workflow used by our team generate data submitting phages Federal Drug...
Here, we report a complex case that involved pediatric patient who experienced recalcitrant multidrug-resistant Pseudomonas aeruginosa infection complicated by bacteremia/sepsis; our antibacterial options were limited because of resistance, allergies, and suboptimal source control. A cocktail 2 bacteriophages targeting the infectious organism introduced on separate occasions sterilized bacteremia.
Abstract In 2016, a 68-year-old patient with disseminated multidrug-resistant Acinetobacter baumannii infection was successfully treated using lytic bacteriophages. Here we report the genomes of nine phages used for treatment and three strains A. isolated prior to during treatment. The in initial are related, T4-like myophages. Analysis 19 isolates collected before phage shows that resistance appeared two days following start We generate complete genomic sequences (TP1, TP2 TP3) treatment,...
Highly concentrated radionuclide waste produced during the Cold War era is stored at US Department of Energy (DOE) production sites. This radioactive was often highly acidic and mixed with heavy metals, has been leaking into environment since 1950s. Because danger expense cleanup such sites by physicochemical processes, in situ bioremediation methods are being developed for contaminated ground groundwater. To date, most microbial treatment proposed high-level employs radiation-resistant...
ABSTRACT Left ventricular assist devices (LVAD) are increasingly used for management of heart failure; infection remains a frequent complication. Phage therapy has been successful in variety antibiotic refractory infections and is interest treating LVAD infections. We performed retrospective review four patients that underwent five separate courses intravenous (IV) phage with concomitant treatment endovascular Pseudomonas aeruginosa infection. assessed susceptibility, bacterial strain...
Skin and soft tissue infections (SSTI) caused by methicillin resistant Staphylococcus aureus (MRSA) are difficult to treat. Bacteriophage (phage) represent a potential alternate treatment for antibiotic bacterial infections. In this study, 7 novel phage with broad lytic activity S. were isolated identified. Screening of diverse collection 170 clinical isolates efficiency plating (EOP) assays shows that the virulent effectively prevent growth 70-91% MRSA sensitive (MSSA) isolates. Phage K,...
To address the need for improved phage annotation tools that scale, we created an automated throughput pipeline: multiple-genome Phage Annotation Toolkit and Evaluator (multiPhATE). multiPhATE is a pipeline driver invokes (PhATE) across user-specified set of genomes. This tool incorporates de novo gene calling algorithm assigns putative functions to calls using protein-, virus- phage-centric databases. multiPhATE's modular construction allows user implement all or any portion analyses by...
Mosquitoes are efficient, militarily relevant vectors of infectious disease pathogens, including many RNA viruses. The vast majority all viruses thought to be undiscovered. Accordingly, recent studies have shown that discovered in insects very divergent from known pathogens and them lack appropriate reference sequences the public databases. Given likely still undiscovered, environ mental sampling stands provide much needed samples as well genetic for comparison. In this study, we sought...
Sequencing-based detection and characterization of viruses in complex samples can suffer from lack sensitivity due to a variety factors including, but not limited to, low titer, small genome size, contribution host or environmental nucleic acids. Hybridization-based target enrichment is one potential method for increasing the viral via high-throughput sequencing. This study expands upon two previously developed panels virus probes (for filoviruses respiratory viruses) include other...
Staphylococcus aureus is the leading cause of skin and soft tissue infections (SSTI). Some S. strains harbor plasmids that carry genes affect resistance to biocides. Among these genes, qacA encodes QacA Multidrug Efflux Pump imparts decreased susceptibility chlorhexidine, a biocide used ubiquitously in healthcare facilities. Furthermore, chlorhexidine has been considered as decolonization strategy community settings. We previously conducted chlorhexidine-based SSTI prevention trial among Ft....
Military trainees are at increased risk for methicillin-resistant Staphylococcus aureus (MRSA) skin and soft tissue infection (SSTI). Whole genome sequencing (WGS) can refine our understanding of MRSA transmission microevolution in congregate settings.We conducted a prospective case-control study SSTI among US Army infantry Fort Benning, Georgia, from July 2012 to December 2014. We identified clusters USA300 within select training classes performed WGS on clinical isolates. then linked...
Transchromosomic bovines (Tc-bovines) adaptively produce fully human polyclonal immunoglobulin (Ig)G antibodies after exposure to immunogenic antigen(s). The National Interagency Confederation for Biological Research and collaborators rapidly produced then evaluated anti-Ebola virus IgG immunoglobulins (collectively termed SAB-139) purified from Tc-bovine plasma sequential hyperimmunization with an Ebola Makona isolate glycoprotein nanoparticle vaccine. SAB-139 was characterized by several...
Human immunodeficiency virus (HIV) infection is characterized by a massive loss of CD4 T cells in the gastrointestinal tract (GIT) that accompanied changes gut microbiome and microbial translocation contribute to inflammation chronic immune activation. Though highly active antiretroviral therapy (HAART) has led better long-term outcomes HIV infected patients, it not been as effective at reverting pathogenesis GIT. Using simian (SIV) model, we show combination (c-ART) partially reverted...
Bacterial infections pose a challenge to human health and burden the care system, especially with spread of antibiotic-resistant populations. To provide effective treatment improved prognosis, diagnostic methods are great importance. Here we present phage-mediated molecular detection (PMMD) as novel method for assessment bacterial antibiotic resistance. This technique consists brief incubation, approximately ten minutes, biological sample natural bacteriophage (phage) targeting bacteria...