A5045497975- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Biosensors and Analytical Detection
- Viral Infections and Outbreaks Research
- Plant Virus Research Studies
- Vibrio bacteria research studies
- Microbial Community Ecology and Physiology
- Bacterial biofilms and quorum sensing
- Lipid Membrane Structure and Behavior
- Bacterial Genetics and Biotechnology
- Infections and bacterial resistance
- Hepatitis B Virus Studies
- Listeria monocytogenes in Food Safety
Hebrew University of Jerusalem
2019-2025
Hadassah Medical Center
2023-2025
A patient with a trauma-related left tibial infection associated extensively drug-resistant Acinetobacter baumannii and multidrug-resistant Klebsiella pneumoniae was treated bacteriophages antibiotics. There rapid tissue healing positive culture eradication. As result, the patient's leg did not have to be amputated he is undergoing rehabilitation.
The use of bacteriophages (phages) is reemerging as a potential treatment option for antibiotic-resistant or nonresolving bacterial infections. Phages are bacteria-specific viruses that may serve personalized therapeutic with minimal collateral damage to the patient microbiome. In 2018 we established Israeli Phage Therapy Center (IPTC) shared initiative Hadassah Medical and Hebrew University Jerusalem, aiming conduct all steps required phage-based solutions, from phage isolation...
Introduction: Pseudomonas aeruginosa is an opportunistic pathogen that causes health care-associated infections. The rise of antibiotic-resistant bacterial strains necessitates alternative treatment strategies, with bacteriophage therapy being a promising approach.
Phage therapy is an experimental therapeutic approach used to target multidrug-resistant bacterial infections. A lack of reliable data with regard its efficacy and regulatory hurdles hinders a broad application. Here we report, for the first time, case vancomycin-resistant Enterococcus faecium abdominal infection in one-year-old, critically ill, three times liver transplanted girl, which was successfully treated intravenous injections (twice per day 20 days) magistral preparation containing...
ABSTRACT Left ventricular assist devices (LVAD) are increasingly used for management of heart failure; infection remains a frequent complication. Phage therapy has been successful in variety antibiotic refractory infections and is interest treating LVAD infections. We performed retrospective review four patients that underwent five separate courses intravenous (IV) phage with concomitant treatment endovascular Pseudomonas aeruginosa infection. assessed susceptibility, bacterial strain...
Antibiotic-resistant pathogens are a growing global issue, leading to untreatable infectious diseases in both humans and animals. Personalized bacteriophage (phage) therapy, the use of specific anti-bacterial viruses, is currently approach combat antibiotic-resistant infections. The implementation phage therapy has primarily been focused on humans, almost neglecting impact such infections health welfare companion Pets also have potential spread resistant their owners or veterinary staff...
A key element in phage therapy is the establishment of large collections, termed herein “banks”, where many well-characterized phages, ready to be used clinic, are stored. These banks serve for both research and clinical purposes. Phage also a microbiology, prior treatment matching phages antibiotics specific bacterial targets. worldwide network can promote phage-based solution any isolated bacteria. Herein, we describe Israeli Bank (IPB) established Hebrew University, Jerusalem, which...
Phage therapy has re-emerged as a promising treatment for non-resolving infections. Given the lack of approved phage treatments, there is need to establish compassionate use pipeline. Here, we present protocol matching, treatment, and monitoring bacteriophage in We describe steps consultation request implementation, evaluating comparing different aspects activity, production. then detail procedures multidisciplinary meetings, ethics approvals, therapy, follow-up. For complete details on...
Abstract Non-resolving bacterial infections involve biofilm formation and are often complicating treatments. Utilizing lytic bacteriophages with antibiotics holds promise in eradication. Accurately matching phage-antibiotic combinations against target bacteria, termed Clinical Phage Microbiology (CPM), is crucial effective phage therapy However, compared to planktonic cultures, performing CPM on poses a significant challenge due the lack of methods standard protocol. This study various...
Background: Antibiotic-resistant Pseudomonas aeruginosa (P. aeruginosa) strains are an increasing cause of morbidity and mortality. Pulsed blue light (PBL) enhances porphyrin-induced reactive oxygen species has been clinically shown to be harmless the skin at low doses. Bacteriophages, viruses that infect bacteria, offer a promising non-antibiotic bactericidal approach. This study investigates potential synergism between low-dose PBL phage therapy against P. in planktonic cultures preformed...
Providencia spp. are emerging pathogens mainly in nosocomial infections. stuartii particular is involved urinary tract infections and contributes significantly to the high incidence of biofilm-formation catheterized patients. Furthermore, recent reports suggested a role for multiple drug resistant (MDR) P. hospital-associated outbreaks which leads excessive complications resulting challenging treatments. Phage therapy currently one most promising solutions combat antibiotic-resistant...
PASA16 is a Pseudomonas aeruginosa phage isolated from soil sample and used to treat several patients suffering persistent infections in various countries. PASA16's genome was sequenced, analyzed, deposited GenBank.
We report the genomic sequences of phages KpCHEMY26 and KpGranit, isolated in Israel during a worldwide effort against multidrug- phage-resistant strain Klebsiella pneumoniae from patient Finland. These results demonstrate importance an efficient network for collaborating personalized therapy infectious diseases.
Phage therapy is a promising antibacterial strategy for resistant respiratory tract infections. inhalation may serve this goal; however, it requires careful assessment of their delivery by approach. Here we present an in vitro model to evaluate phage inhalation. Eight phages, most which target pathogens common cystic fibrosis, were aerosolised jet nebuliser and administered real-scale computed tomography-derived 3D airways with breathing simulator. Viable loads reaching the output tracheal...
Abstract Providencia spp . are emerging pathogens mainly in nosocomial infections. stuartii particular is involved urinary tract infections and contributes significantly to the high incidence of biofilm-formation catheterized patients. Furthermore, recent reports suggested a role for multiple drug resistant (MDR) P. hospital-associated outbreaks which leads excessive complications resulting challenging treatments. Phage therapy currently one most promising solutions combat...
Abstract Antibiotic-resistant Pseudomonas aeruginosa (PA) is a critical health threat. Novel treatment approaches are urgently required in this post-antibiotic era. In the current study, we investigated bactericidal combinatorial potential of two non-antibiotic alternative approaches: phage therapy and pulsed blue light (PBL). Bacteriophages (phages), viruses that specifically infect lyse bacteria without harming eukaryotic cells. Pulsed (PBL) alters bacterial membranes was clinically shown...
Abstract Introduction Pseudomonas aeruginosa is a major opportunistic pathogen associated with healthcare-associated infections. The rise of antibiotic-resistant strains necessitates alternative treatment strategies, bacteriophage therapy being promising approach. Methods Six bacteriophages were isolated from sewage samples. Phage isolation involved centrifugation, filtration, and plaque assays. morphology each was examined using Transmission Electron Microscopy (TEM). Genomic DNA sequenced...
EFGrKN and EFGrNG are new Enterococcus faecalis phages that were isolated from sewage samples as part of the Israeli Phage Bank (IPB). The complete genomes sequenced, analyzed, deposited in GenBank. According to their lytic activity vitro , it seems these have a potential be used future phage therapy treatments.
Abstract Personalized-phage-therapy is a promising solution for the emerging crisis of bacterial infections that fail to be eradicated by conventional antibiotics. One most crucial elements personalized-phage-therapy proper matching phages and antibiotics target bacteria in given clinical setting. However, date, there no consensus guideline laboratory procedures enable vitro evaluation intended treatment. In this work, we suggest framework strategies identify appropriate combine them with...
Abstract Phage therapy is a promising antibacterial strategy for resistant respiratory tract infections. inhalation may serve this goal; however, it requires careful assessment of their delivery by approach. Here we present an in-vitro model to evaluate phage inhalation. Eight phages, most which target CF-common pathogens, were aerosolized and administered real-scale CT□derived 3D airways with breathing simulator. Viable loads reaching the output nebulizer tracheal level determined compared...