A5014389259- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Antibiotic Use and Resistance
- Antimicrobial Resistance in Staphylococcus
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Urinary Tract Infections Management
- Antibiotics Pharmacokinetics and Efficacy
- Pharmaceutical and Antibiotic Environmental Impacts
- Infection Control in Healthcare
- Mycobacterium research and diagnosis
- Enterobacteriaceae and Cronobacter Research
- Bacillus and Francisella bacterial research
- COVID-19 Clinical Research Studies
- Genomics and Phylogenetic Studies
- Viral Infections and Outbreaks Research
- Patient Satisfaction in Healthcare
- COVID-19 and healthcare impacts
- Infections and bacterial resistance
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Tuberculosis Research and Epidemiology
- Escherichia coli research studies
- Viral Infections and Vectors
- Molecular Biology Techniques and Applications
Unity Health System
2017-2024
Rochester General Hospital
2020-2024
Allegheny General Hospital
2022
Museum of Heilongjiang Province
2021
SUNY Upstate Medical University
2020
Walter Reed Army Institute of Research
2010-2019
Engineering Associates (United States)
2018
Uniformed Services University of the Health Sciences
2011-2017
United States Naval Medical Research Unit SOUTH
2017
Naval Medical Research Command
2017
Acinetobacter baumannii is recognized as an emerging bacterial pathogen because of traits such prolonged survival in a desiccated state, effective nosocomial transmission, and inherent ability to acquire antibiotic resistance genes. A pressing need the field A. research suitable model strain that representative current clinical isolates, highly virulent established animal models, can be genetically manipulated. To identify strain, diverse set recent U.S. military isolates was assessed....
Within the paradigm of clinical infectious disease research, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa represent four most clinically relevant, hence extensively studied bacteria. Current culture-based methods for identifying these organisms are slow cumbersome, there is increasing need more rapid accurate molecular detection methods. Using bioinformatic tools, 962,279 bacterial 16S rRNA gene sequences were aligned, regions homology selected...
Background. Colistin resistance is of concern since it increasingly needed to treat infections caused by bacteria resistant all other antibiotics and has been associated with poorer outcomes. Longitudinal data from in vivo series are sparse. Methods. Under a quality-improvement directive intensify infection-control measures, extremely drug-resistant (XDR) undergo phenotypic molecular analysis. Results. Twenty-eight XDR Acinetobacter baumannii isolates were longitudinally recovered during...
Abstract Background Infections by pan-drug resistant Acinetobacter baumannii plague military and civilian healthcare systems. Previous A. pan-genomic studies used modest sample sizes of low diversity comparisons to a single reference genome, limiting our understanding gene order content. A consensus representation multiple genomes will provide better framework for comparison. large-scale comparative study identify genomic determinants associated with their adaptation as successful pathogen....
ABSTRACT The alarming rise in antibiotic resistance has led to an increase patient mortality and health care costs. This problem is compounded by the absence of new antibiotics close regulatory approval. Acinetobacter baumannii a human pathogen that causes infections primarily patients intensive units (ICUs) highly resistant. Colistin one last-line for treating A. infections; however, colistin-resistant strains are becoming increasingly common. cationic attacks negatively charged bacterial...
Background. Severe Acinetobacter baumannii infections in immunocompetent patients are uncommon, and the virulence mechanisms of this organism not fully understood. Methods. Following an outbreak fatal A. a cohort relatively (low comorbidity illness severity scores), isolates were investigated with comparative genomics animal models. Results. Two unrelated clades associated outbreak. The clone majority patient deaths, clade B, is evolutionarily distinct from 3 international clonal complexes,...
ABSTRACT In response to global concerns over the spread of New Delhi metallo-β-lactamase gene 1, bla NDM-1 , a monthly surveillance program was initiated in September 2010. All carbapenem-resistant Gram-negative strains forwarded our facility are screened for this gene. To date, 321 isolates, encompassing 11 bacterial species, have been tested. February 2011, two Providencia stuartii submitted from military hospital Afghanistan, tested positive . Both were identical by pulsed-field gel...
ABSTRACT Gene amplification is believed to play an important role in antibiotic resistance but has been rarely documented clinical settings because of its unstable nature. We report a rise MICs from 0.5 16 μg/ml successive Acinetobacter baumannii isolated over 4 days patient being treated with tobramycin for infection by multidrug-resistant A. , resulting therapeutic failure. Isolates were characterized whole-genome sequencing, real-time and reverse transcriptase PCR, growth assays determine...
The emergence of a transferable colistin resistance gene (mcr-1) is global concern. insertion sequence ISApl1 key component in the mobilization this gene, but its role remains poorly understood. Six Escherichia coli isolates were cultured from same patient over course 1 month Germany and United States after brief hospitalization Bahrain for an unconnected illness. Four carried mcr-1 as determined by real-time PCR, two negative. Two additional mcr-1-negative E. collected during follow-up...
Following a fatal intensive care unit (ICU) outbreak of carbapenem-resistant Acinetobacter baumanii (CRAB) in 2015, an aggressive infection control intervention was instituted. We outline the and long-term changes incidence prevalence CRAB.The included closure (3 days), environmental cleaning, hand hygiene interventions, culturing. CRAB acquisition colistin use were compared for 1 year before 2 years after intervention.Following intervention, ICU decreased significantly from 54.6...
Over the past two decades, Acinetobacter baumannii has emerged as a leading cause of nosocomial infections worldwide. Of particular concern are panresistant strains, World Health Organization (WHO) to designate carbapenem-resistant A. priority 1 (critical) pathogen for research and development new antibiotics. A key component in supporting this effort is accessibility diverse clinically relevant strains testing. Here, we describe panel 100 use endeavor. Whole-genome sequencing was performed...
are a leading cause of healthcare-associated infections worldwide. In particular, strains expressing extended-spectrum β-lactamases (ESBLs) and carbapenemases pose serious treatment challenges, the World Health Organization (WHO) to designate ESBL carbapenem-resistant Enterobacteriaceae as 'critical' threats human health. Research efforts combat these pathogens can be supported by accessibility diverse clinically relevant isolates for testing novel therapeutics. Here, we describe panel 100
ABSTRACT Klebsiella pneumoniae strain MRSN2404 was isolated from the chronic wound of a soldier who had been wounded in Iraq 2006. The displayed very high MICs all aminoglycosides, including arbekacin. A gene encoding novel 16S rRNA methyltransferase, now designated RmtH, identified. RmtH 64% identity with RmtB1 and RmtB2. rmtH bracketed by two copies IS CR2 , which may have played role its mobilization.
Tigecycline nonsusceptibility is concerning because tigecycline increasingly relied upon to treat carbapenem- or colistin-resistant organisms. In Enterobacteriaceae, mediated by the AcrAB-TolC efflux pump, among others, and pump activity often a downstream effect of mutations in their transcriptional regulators, cognate repressor genes, noncoding regions, as demonstrated Enterobacteriaceae Acinetobacter isolates. Here, we report emergence longitudinal series multidrug-resistant (MDR)...
Abstract Objectives Pseudomonas aeruginosa is a leading cause of community- and hospital-acquired infections. Successful treatment hampered by its remarkable ability to rapidly develop resistance antimicrobial agents, primarily through mutation. In response, WHO listed carbapenem-resistant P. as Priority 1 (Critical) pathogen for research development new treatments. A key resource in developing effective countermeasures access diverse clinically relevant strains testing. Herein we describe...
Responding to escalating antimicrobial resistance (AMR), the US Department of Defense implemented an enterprise-wide collaboration, Antimicrobial Resistance Monitoring and Research Program, aid in infection prevention control. It consists a network epidemiologists, bioinformaticists, microbiology researchers, policy makers, hospital-based preventionists, healthcare providers who collaborate collect relevant AMR data, conduct centralized molecular characterization, use characterization...
Whole genome sequencing (WGS) is increasingly employed in clinical settings, though few assessments of turnaround times (TAT) have been performed real-time. In this study, WGS was used to investigate an unfolding outbreak vancomycin resistant Enterococcus faecium (VRE) among 3 patients the ICU a tertiary care hospital. Including overnight culturing, TAT just 48.5 h for comprehensive report achievable using Illumina Miseq benchtop sequencer. revealed that isolates from patient 2 and differed...
Background Gram-negative multidrug-resistant (MDR) bacteria are major causes of nosocomial infections, and antibiotic resistance in these organisms is often plasmid mediated. Data scarce pertaining to molecular mechanisms resource constrained areas such as Iraq. Methodology/Principal Findings In this study, all MDR Enterobacteriaceae (n = 38) randomly selected non-MDR counterparts 41) isolated from patients, healthcare workers environmental surfaces a newly opened hospital Iraq were...
ABSTRACT A 22-year-old male developed a recurrent sacral abscess associated with embedded shrapnel following blast injury. Cultures grew extended-spectrum β-lactamase (ESBL)-producing, carbapenem-susceptible Escherichia coli . Ertapenem was administered, but the infection recurred after each course of antibiotics. Initial surgical interventions were unsuccessful, and subsequent cultures yielded E. Morganella morganii , both nonsusceptible to carbapenems. The isolates Carba NP test negative,...