A5057845837- Bacteriophages and microbial interactions
- Bacillus and Francisella bacterial research
- Brucella: diagnosis, epidemiology, treatment
- Microbial infections and disease research
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Yersinia bacterium, plague, ectoparasites research
- Burkholderia infections and melioidosis
- Monoclonal and Polyclonal Antibodies Research
- Escherichia coli research studies
- Plant Virus Research Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Zoonotic diseases and public health
- Animal Diversity and Health Studies
- Identification and Quantification in Food
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial Identification and Susceptibility Testing
- Viral Infections and Vectors
- Antimicrobial Resistance in Staphylococcus
- Vector-borne infectious diseases
- Galectins and Cancer Biology
- Bacterial biofilms and quorum sensing
- Salmonella and Campylobacter epidemiology
Walter Reed Army Institute of Research
2015-2025
United States Military Academy
2019
United States Army
2015-2017
Agricultural University of Georgia
2015
University of Georgia
2015
Tbilisi State University
2015
National Center for Disease Control and Public Health
2015
Special Olympics
2012
University of Chicago
2010
University of Illinois Urbana-Champaign
1992-1994
Bacteriophages specific for Yersinia pestis are routinely used plague diagnostics and could be an alternative to antibiotics in case of drug-resistant plague. A major concern bacteriophage therapy is the emergence phage-resistant mutants. The use phage cocktails can overcome this problem but only if phages exploit different receptors. Some mutants lose virulence therefore should not complicate therapy.The purpose work was identify Y. receptors using site-directed mutagenesis...
Background. Colistin resistance is of concern since it increasingly needed to treat infections caused by bacteria resistant all other antibiotics and has been associated with poorer outcomes. Longitudinal data from in vivo series are sparse. Methods. Under a quality-improvement directive intensify infection-control measures, extremely drug-resistant (XDR) undergo phenotypic molecular analysis. Results. Twenty-eight XDR Acinetobacter baumannii isolates were longitudinally recovered during...
Abstract Background Infections by pan-drug resistant Acinetobacter baumannii plague military and civilian healthcare systems. Previous A. pan-genomic studies used modest sample sizes of low diversity comparisons to a single reference genome, limiting our understanding gene order content. A consensus representation multiple genomes will provide better framework for comparison. large-scale comparative study identify genomic determinants associated with their adaptation as successful pathogen....
Multidrug-resistant (MDR) Pseudomonas aeruginosa infections pose a serious health threat. Bacteriophage–antibiotic combination therapy is promising candidate for combating these infections. A 5-phage P. cocktail, PAM2H, was tested in with antibiotics (ceftazidime, ciprofloxacin, gentamicin, meropenem) to determine if PAM2H enhances antibiotic activity. Combination treatment vitro resulted significant increase susceptibility of MDR strains antibiotics. Treatment ceftazidime (CAZ), meropenem,...
ABSTRACT Left ventricular assist devices (LVAD) are increasingly used for management of heart failure; infection remains a frequent complication. Phage therapy has been successful in variety antibiotic refractory infections and is interest treating LVAD infections. We performed retrospective review four patients that underwent five separate courses intravenous (IV) phage with concomitant treatment endovascular Pseudomonas aeruginosa infection. assessed susceptibility, bacterial strain...
Though numerous studies have shown that gene transfer occurs between distantly related bacterial genera under laboratory conditions, the frequency and breadth of horizontal events in nature remain unknown. Previous evidence for natural intergeneric transfers came from genes human pathogens, bacteria colonize same host. We present a tetracycline resistance gene, tetQ, has occurred normally different hosts. A DNA sequence comparative approach was taken to examine extent tetQ dissemination...
To gain insights into the origin and genome evolution of plague bacterium Yersinia pestis, we have sequenced deep-rooted strain Angola, a virulent Pestoides isolate. Its ancient nature makes this atypical isolate particular importance in understanding pathogenicity. chromosome features unique genetic make-up intermediate between modern Y. pestis isolates its evolutionary ancestor, pseudotuberculosis. Our genotypic phenotypic analyses led us to conclude that Angola belongs one most lineages...
To determine the anatomic sites and natural history of colonization with gram-negative multidrug-resistant organisms (MDROs).Prospective, longitudinal cohort study.Walter Reed Army Medical Center, a 236-bed tertiary care center in Washington, DC.Deployed subjects (ie, inpatients medically evacuated from Iraq or Afghanistan) nondeployed admitted to same hospital.Consenting patients had 6 cultured every 3 days for 2 weeks then weekly. Gram-negative resistant more classes antibiotics were...
Abstract Background Bacteriophages (phages) are a promising anti-infective option for human disease. Major gaps remain in understanding their potential utility. Methods This is randomized, placebo-controlled, double-blind study of single dose intravenous phage approximately 72 clinically stable adult cystic fibrosis volunteers recruited from up to 20 US sites with Pseudomonas aeruginosa airway colonization. The consists mixture four anti-pseudomonal phages. Six sentinel participants will be...
Brucellae are gram-negative intracellular pathogens that survive and multiply within host phagocytic cells. Smooth organisms present O-polysaccharides (OPS) on their surface. The wboA gene, which codes for the enzyme glycosyl transferase, is essential assembly of O-chain in Brucella. Deletion smooth, virulent B. melitensis 16M results a rough mutant designated WRR51. Unlike abortus, both smooth strains resistant to complement-mediated killing. To determine role surface OPS interactions with...
The ribosome protection type of tetracycline resistance (Tcr) has been found in a variety bacterial species, but the only two classes described previously, Tet(M) and Tet(O), shared high degree amino acid sequence identity (greater than 75%). Thus, it appeared that this emerged recently evolution spread among different species bacteria by horizontal transmission. We obtained DNA Tcr gene from Bacteroides, genus gram-negative, obligately anaerobic is phylogenetically distant diverse which...
Brucellosis is an endemic disease in the country of Georgia. According to National Center for Disease Control and Public Health Georgia (NCDC), average annual number brucellosis cases was 161 during 2008-2012. However, true thought be higher due underreporting. The aim this study provide current epidemiological clinical information evaluate diagnostic methods used Georgia.Adult patients were eligible participation if they met suspected or probable case definition brucellosis. After consent...
Staphylococci are frequent agents of health care-associated infections and include methicillin-resistant Staphylococcus aureus (MRSA), which is resistant to first-line antibiotic treatments. Bacteriophage (phage) therapy a promising alternative antibacterial option treat MRSA infections. S. aureus-specific phage Sb-1 has been widely used in Georgia variety human broad host range within aureus, including strains, its can be further expanded by adaptation previously clinical isolates. The...
Background Yersinia pestis, the agent of plague, has caused many millions human deaths and still poses a serious threat to global public health. Timely reliable detection such dangerous pathogen is critical importance. Lysis by specific bacteriophages remains an essential method Y. pestis plague diagnostics. Methodology/Principal Findings The objective this work was develop alternative conventional phage lysis tests – rapid highly sensitive indirect live cells based on quantitative real-time...
We analysed both pooled and individual tick samples collected from four countries in Eastern Europe the Black Sea region, using metagenome-based nanopore sequencing (NS) targeted amplification. Initially, 1337 ticks, belonging to 11 species, were screened 217 pools. Viruses (21 taxa) human pathogens detected 46.5% 7.3%, respectively. Tick-borne viral comprised Tacheng Tick Virus 2 (TTV2, 5.9%), Jingmen (JMTV, 0.9%) 1 (TTV1, 0.4%). An association of species with virus taxa was observed,...
Phage therapeutics offer a potentially powerful approach for combating multidrug-resistant bacterial infections. However, to be effective, phage therapy must overcome existing and developing resistance. While cocktails can reduce this risk by targeting multiple receptors in single therapeutic, bacteria have mechanisms of resistance beyond receptor modification. A rapidly growing body knowledge describes broad varied arsenal antiphage systems encoded counter infection. We sought understand...
Background Gram-negative multidrug-resistant (MDR) bacteria are major causes of nosocomial infections, and antibiotic resistance in these organisms is often plasmid mediated. Data scarce pertaining to molecular mechanisms resource constrained areas such as Iraq. Methodology/Principal Findings In this study, all MDR Enterobacteriaceae (n = 38) randomly selected non-MDR counterparts 41) isolated from patients, healthcare workers environmental surfaces a newly opened hospital Iraq were...
ABSTRACT We describe the genomes of three lytic Pseudomonas aeruginosa phages genus Phikmvvirus . The vB_Pae4841-AFR43, vB_Pae10145-KEN1, and vB_Pae9718-KEN10 consist 43,426, 43,406, 43,118 bp, with 62.4%, 62.3%, 62.2% GC content, contain 63, 66, 64 coding sequences, respectively, no tRNA genes.
ABSTRACT We describe the genomes of two Pseudomonas aeruginosa phages genus Bruynoghevirus , WRAIR_EPa83 and WRAIR_EPa87. They consist 45,622 45,077 bp, with 52.52% 52.11% guanine-cytosine content, contain 81 80 coding sequences, three tRNA genes, direct terminal repeats 183 184 respectively.
ABSTRACT Brucella spp. are gram-negative intracellular pathogens that survive and multiply within phagocytic cells of their hosts. Smooth organisms present O polysaccharides (OPS) on surface. These OPS help the bacteria avoid bactericidal action serum. The wboA gene, coding for enzyme glycosyltransferase, is essential synthesis chain in . In this study, sensitivity to serum smooth, virulent melitensis 16M B. abortus 2308, rough mutants VTRM1, RA1, WRR51 derived from these two species,...