A5040266802- Orthopedic Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Infective Endocarditis Diagnosis and Management
- Bacterial biofilms and quorum sensing
- Orthopaedic implants and arthroplasty
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Total Knee Arthroplasty Outcomes
- Wound Healing and Treatments
- Antibiotic Use and Resistance
- Streptococcal Infections and Treatments
- Antimicrobial agents and applications
- Antibiotics Pharmacokinetics and Efficacy
- Planarian Biology and Electrostimulation
- Microbial infections and disease research
- Legionella and Acanthamoeba research
- Bacteriophages and microbial interactions
- Infectious Diseases and Tuberculosis
- Mycobacterium research and diagnosis
- Clostridium difficile and Clostridium perfringens research
- Antimicrobial Peptides and Activities
- Antifungal resistance and susceptibility
- Neonatal and Maternal Infections
- Plant Virus Research Studies
- Neonatal Health and Biochemistry
Mayo Clinic
2016-2025
Mayo Clinic in Arizona
2016-2025
WinnMed
2013-2024
Mayo Clinic in Florida
2014-2023
University of Minnesota Rochester
2016-2020
Engineering Associates (United States)
2018
University of Rochester
2012
ABSTRACT Periprosthetic tissue and/or synovial fluid PCR has been previously studied for prosthetic joint infection (PJI) diagnosis; however, few studies have assessed the utility of on biofilms dislodged from surface explanted arthroplasties using vortexing and sonication (i.e., sonicate PCR). We compared 16S rRNA gene real-time sequencing to culture fluid, tissue, microbiologic diagnosis PJI. sequences generating mixed chromatograms were decatenated RipSeq Mixed. fluids 135 231 subjects...
Metagenomic shotgun sequencing has the potential to change how many infections, particularly those caused by difficult-to-culture organisms, are diagnosed. Metagenomics was used investigate prosthetic joint infections (PJIs), where pathogen detection can be challenging. Four hundred eight sonicate fluid samples generated from resected hip and knee arthroplasties were tested, including 213 subjects with 195 without infection. Samples enriched for microbial DNA using MolYsis basic kit,...
We previously showed that culture of samples obtained by prosthesis vortexing and sonication was more sensitive than tissue for prosthetic joint infection (PJI) diagnosis. Despite improved sensitivity, culture-negative cases remained; furthermore, has a long turnaround time. designed genus-/group-specific rapid PCR assay panel targeting PJI bacteria applied it to sonicating explanted hip knee prostheses, we compared the results those with sonicate fluid periprosthetic at revision or...
Despite known low sensitivity, culture of periprosthetic tissue specimens on agars and in broths is routine. Culture samples blood bottles (BCBs) potentially more convenient, but it has been evaluated a limited way not widely adopted. The aim this study was to compare the sensitivity specificity inoculation into with standard agar thioglycolate broth culture, applying Bayesian latent class modeling (LCM) addition Infectious Diseases Society America (IDSA) criteria for prosthetic joint...
Metagenomic shotgun sequencing has the potential to transform how serious infections are diagnosed by offering universal, culture-free pathogen detection. This may be especially advantageous for microbial diagnosis of prosthetic joint infection (PJI) synovial fluid analysis since cultures not universally positive and is easily obtained preoperatively.
Small colony variants (SCVs) are naturally occurring subpopulations of bacteria. The clinical characteristics and treatment outcomes patients with prosthetic joint infection (PJI) caused by staphylococcal SCVs unknown. This study was a retrospective series 113 PJI, prospective testing archived sonicate fluid samples. were defined using two-investigator review. Treatment failure as (i) subsequent revision surgery for any reason, (ii) PJI after the index surgery, (iii) prosthesis...
ABSTRACT We recently demonstrated improved sensitivity of prosthetic joint infection (PJI) diagnosis using an automated blood culture bottle system for periprosthetic tissue [T. N. Peel et al., mBio 7(1):e01776-15, 2016, https://doi.org/10.1128/mBio.01776-15 ]. This study builds on the prior research by examining optimal number specimens required accurate PJI diagnosis. Current guidelines recommend five to six, which is impractical. applied Bayesian latent class modeling techniques...
Abstract Background Next-generation sequencing (NGS) is increasingly used for periprosthetic joint infection (PJI) diagnosis, but its clinical utility poorly defined. Shotgun metagenomic (sNGS) has been reported to identify PJI pathogens undetected by culture in sonicate fluid. However, sNGS complex and costly. Here, 16S ribosomal RNA (rRNA) gene-based targeted (tNGS) was compared of fluid microbial detection identification patients with total hip arthroplasty (THA) knee (TKA) failure....
Defining the microbial etiology of culture-negative prosthetic joint infection (PJI) can be challenging. Metagenomic shotgun sequencing is a new tool to identify organisms undetected by conventional methods. We present case where metagenomics was used Mycoplasma salivarium as novel PJI pathogen in patient with hypogammaglobulinemia.
ABSTRACT Whole-genome amplification (WGA) is a useful tool for of very small quantities DNA many uses, including metagenomic shotgun sequencing infection diagnosis. Depending on the application, background from WGA kits can be problematic. Three were tested their utility in metagenomics approach to identify pathogens sonicate fluid comprised biofilms and other materials dislodged surfaces explanted prosthetic joints using sonication. The Illustra V2 Genomiphi, single cell Qiagen REPLI-g used...
Staphylococcus aureus is a leading cause of bacteremia in hospitalized patients. Whether or not S. (SAB) associated with clonality, implicating potential nosocomial transmission, has not, however, been investigated. Herein, we examined the epidemiology SAB using whole genome sequencing (WGS). 152 isolates collected over course 2015 at single large Minnesota medical center were studied. protein A (spa) typing was performed by PCR/Sanger sequencing; multilocus sequence (MLST) and core MLST...
The diagnosis of periprosthetic joint infection (PJI) is challenging, often requiring multiple clinical specimens and diagnostic techniques, some with prolonged result turnaround times. Here, the performance Investigational Use Only (IUO) BioFire Joint Infection (JI) Panel was compared to 16S rRNA gene-based targeted metagenomic sequencing (tMGS) applied synovial fluid for PJI diagnosis. Sixty samples from knee arthroplasty failure archived at -80°C were tested. Infectious Diseases Society...
Abstract Background Bacteriophages (phages) are a promising anti-infective option for human disease. Major gaps remain in understanding their potential utility. Methods This is randomized, placebo-controlled, double-blind study of single dose intravenous phage approximately 72 clinically stable adult cystic fibrosis volunteers recruited from up to 20 US sites with Pseudomonas aeruginosa airway colonization. The consists mixture four anti-pseudomonal phages. Six sentinel participants will be...
ABSTRACT The growing threat of antibiotic-resistant bacterial pathogens necessitates the development alternative antimicrobial approaches. This is particularly true for chronic wound infections, which commonly harbor biofilm-dwelling bacteria. A novel electrochemical bandage (e-bandage) delivering low-levels hypochlorous acid (HOCl) was evaluated against Pseudomonas aeruginosa murine biofilms. 5 mm skin wounds were created on dorsum mice and infected with 10 6 colony-forming units (CFU) P. ....
ABSTRACT The activity of a novel β-lactamase inhibitor combination, sulbactam-durlobactam (SUL-DUR), was tested against 87 colistin-resistant and/or cefiderocol-non-susceptible carbapenem-resistant Acinetobacter baumannii clinical isolates collected from U.S. hospitals between 2017 and 2019. Among them, 89% 97% were susceptible to SUL-DUR imipenem plus SUL-DUR, with MIC 50 /MIC 90 values 2 µg/mL/8 µg/mL 1 µg/mL/4 µg/mL, respectively. presence amino acid substitutions in penicillin-binding...
Identification of Corynebacterium species may be challenging. are occasional causes prosthetic joint infection (PJI), but few data available on the subject. Based literature, C. amycolatum, aurimucosum, jeikeium, and striatum most common that cause PJI. We designed a rapid PCR assay to detect human species, with specific focus A polyphosphate kinase gene identified using whole-genome sequence was targeted. The differentiates antibiotic-resistant jeikeium urealyticum from other in single...
Although carbapenems are effective for treating serious multidrug-resistant Pseudomonas aeruginosa infections, carbapenem-resistant P. (CRPA) is now being reported worldwide. Ceftolozane-tazobactam (C/T) demonstrates activity against many isolates. We evaluated the of C/T and compared its to that ceftazidime-avibactam (C/A) using a well-characterized collection non-carbapenemase-producing CRPA Forty-two isolates from previous study (J. Y. Lee K. S. Ko, Int J Antimicrob Agents 40:168-172,...
We have previously demonstrated that culturing periprosthetic tissue in blood culture bottles (BCBs) improves sensitivity compared to conventional agar and broth methods for diagnosis of prosthetic joint infection (PJI). also shown prosthesis sonication using methods. The purpose this study was compare the diagnostic accuracy BCBs (subsequently referred as culture) sonicate fluid culture). studied 229 subjects who underwent arthroplasty revision or resection surgery between March 2016...