A5103046449- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Gene expression and cancer classification
- Microbial Metabolic Engineering and Bioproduction
- Protein purification and stability
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- Microfluidic and Capillary Electrophoresis Applications
- Bacillus and Francisella bacterial research
- Microbial Inactivation Methods
- Microfluidic and Bio-sensing Technologies
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Structure and Dynamics
- Phytase and its Applications
- Heat shock proteins research
- Enzyme Catalysis and Immobilization
- Bacterial Identification and Susceptibility Testing
- Redox biology and oxidative stress
- Biopolymer Synthesis and Applications
- Pharmacogenetics and Drug Metabolism
- Endoplasmic Reticulum Stress and Disease
- Iron Metabolism and Disorders
- Genomics and Phylogenetic Studies
Universität Greifswald
2007-2023
Universitätsmedizin Greifswald
2019
Universität Hamburg
2006
Fraunhofer Institute for Silicon Technology
2006
Low-density electrical 16S rRNA specific oligonucleotide microarrays and an automated analysis system have been developed for the identification quantitation of pathogens. The pathogens are Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, epidermidis, which typically involved in urinary tract infections. Interdigitated gold array electrodes (IDA-electrodes), structures nanometer range, used very sensitive analysis. Thiol-modified oligonucleotides...
The cellular response of Escherichia coli to overproduction the insoluble heterologous protein α-glucosidase Saccharomyces cerevisiae during a glucose-limited fed-batch fermentation was analyzed on transcriptional and translational levels. After induction tac-regulated overexpression recombinant model protein, significant but transient increase mRNA levels heat shock genes lon dnaK could be observed. level gene coding for inclusion body-associated IbpB showed strongest remained at clearly...
Bacterial inclusion bodies (IBs) are key intermediates for protein production. Their quality affects the refolding yield and further purification. Recent functional structural studies have revealed that IBs not dead-end aggregates but undergo dynamic changes, including aggregation, refunctionalization of proteolysis. Both, aggregation folding turnover influenced by cellular situation a number well-studied chaperones proteases included. mostly contain only minor impurities relatively...
The Gram-positive endospore-forming bacterium Bacillus licheniformis can be found widely in nature and it is exploited industrial processes for the manufacturing of antibiotics, specialty chemicals, enzymes. Both its varied natural habitats settings, B. cells will exposed to increases external osmolarity, conditions that trigger water efflux, impair turgor, cause cessation growth, negatively affect productivity cell factories biotechnological processes. We have taken here both systems-wide...
Abstract The glucose and nitrogen starvation stimulons of Bacillus licheniformis were determined by transcriptome proteome analyses. Under both conditions, the main response B. was a switch to usage alternative nutrient sources. This indicated an induction genes involved in metabolism C‐2 substrates during limitation. In addition, seems be using other organic substances like amino acids lipids as carbon sources when subjected starvation. observation is supported high number coding for...
Bacillus pumilus is characterized by a higher oxidative stress resistance than other comparable industrially relevant Bacilli such as B. subtilis or licheniformis. In this study the response of to was investigated during treatment with high concentrations hydrogen peroxide at proteome, transcriptome and metabolome level. Genes/proteins belonging regulons, which are known have important functions in organisms, were found be upregulated, Fur, Spx, SOS CtsR regulon. Strikingly, parts...
The oxidative stress response of Bacillus licheniformis after treatment with hydrogen peroxide was investigated at the transcriptome, proteome and metabolome levels. In this comprehensive study, 84 proteins 467 transcripts were found to be up or downregulated in stressor. Among upregulated genes many that are known have important functions other organisms, such as catalase, alkylhydroperoxide reductase thioredoxin system. Many these could grouped into putative regulons by genomic mining....
Abstract A detailed gene expression analysis of industrial‐close Bacillus subtilis fed‐batch fermentation processes with casamino acids as the only nitrogen source and a reduced acid concentration but supplemented by ammonia was carried out. Although glutamine arginine are supposed to be preferred sources B. , we demonstrate that combined feeding supports cell growth under conditions. The transcriptome proteome analyses revealed additional in combination amino results significantly lower...
The phosphate-starvation stimulon of Bacillus licheniformis was analyzed at the transcriptional and translational level. comparison transcriptome proteome demonstrated that this specific starvation response B. is partially similar to subtilis. However, it also shown has evolved its own strategies cope with nutrient limitation. By means secretome analysis phytase identified as most abundant protein under conditions. Data study indicate that, unlike in subtilis, phosphate does not induce...
The heat and ethanol stress response of Bacillus licheniformis DSM13 was analyzed at the transcriptional and/or translational level. During shock, regulons known to be heat-induced in subtilis 168 are upregulated B. licheniformis, such as HrcA, SigB, CtsR, CssRS regulon. Upregulation SigY regulon genes controlled by other extracytoplasmic function (ECF) sigma factors indicates a cell-wall triggered shock. Furthermore, tryptophan synthesis enzymes were stressed cells well involved usage...
The knowledge of critical process-relevant genes can be used for an improved control bioprocesses. So far bioprocess-relevant marker analyzed by established expression analysis methods only off-line. In this study, alternative approach a potential at-line monitoring gene during bioprocesses is suggested. This based on the measurement specific mRNAs electric DNA-chip in connection with magnetic bead-based sandwich hybridization. order to allow method fast and partially automated isolation...
Abstract Thiol‐disulfide oxidoreductases of the thioredoxin superfamily are crucial for maintaining thiol redox state in living organisms. For bacterium Bacillus subtilis A (TrxA) was described as product an essential gene indicating a key role during growth. By means mRNA profiling Smits et al. ( J. Bacteriol. 2005, 187 , 3921–3930) suggested critical function TrxA sulfur utilization stationary phase. We extended analysis to exponential growth and characterized trxA conditional mutant by...
A promoter that drives high-level, long-term expression of the target gene under substrate limited growth conditions in absence an artificial inducer would facilitate efficient production heterologous proteins at low cost. novel phosphate-regulated system was constructed using phytase encoding phyL from Bacillus licheniformis for overexpression this industrially relevant host. It is shown enables a strong genes amyE and xynA B. when cells were subjected to phosphate limitation. Whether can...