A5056422039- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Enzyme Structure and Function
- Enzyme Production and Characterization
- Probiotics and Fermented Foods
- Analytical Chemistry and Sensors
- Electrochemical sensors and biosensors
- Amino Acid Enzymes and Metabolism
- Bacteriophages and microbial interactions
- Protein Hydrolysis and Bioactive Peptides
- X-ray Diffraction in Crystallography
- Bacillus and Francisella bacterial research
- RNA and protein synthesis mechanisms
- Crystallization and Solubility Studies
- Photosynthetic Processes and Mechanisms
- Chemical Synthesis and Analysis
- Advanced Chemical Sensor Technologies
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Chromatography
- Hemoglobin structure and function
- Metabolism and Genetic Disorders
- Microbial metabolism and enzyme function
- Peptidase Inhibition and Analysis
FH Aachen
2014-2025
Stadtwerke Jülich (Germany)
2020
Digital Proteomics (United States)
2015
RWTH Aachen University
2014
Henkel (Germany)
2009-2013
DSM (Netherlands)
2000-2002
Forschungszentrum Jülich
2001
Johannes Gutenberg University Mainz
1994-1999
University of Applied Sciences Mainz
1995
Heinrich Heine University Düsseldorf
1995
Bacillus subtilis and licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into culture medium using general secretion (Sec) pathway. A characteristic feature all secreted is their N-terminal signal peptides, which recognized by machinery. Here, we have studied an industrially important protease, namely, subtilisin BPN' from amyloliquefaciens. One hundred seventy-three peptides originating B. 220 type strain were...
Abstract N-Acyl-amino acids can act as mild biobased surfactants, which are used, e.g., in baby shampoos. However, their chemical synthesis needs acyl chlorides and does not meet sustainability criteria. Thus, the identification of biocatalysts to develop greener routes is desirable. We describe a novel aminoacylase from Paraburkholderia monticola DSM 100849 (PmAcy) was identified, cloned, evaluated for its N-acyl-amino acid potential. Soluble protein obtained by expression lactose...
In Escherichia coli the expression of nuo genes encoding proton pumping NADH dehydrogenase I is stimulated by presence fumarate during anaerobic respiration. The regulatory sites required for induction fumarate, nitrate and O 2 are located at positions around –309, –277, downstream –231 bp, respectively, relative to transcriptional‐start site. regulator has be different from O2 regulators ArcA NarL. For growth respiration, was essential, in contrast aerobic or respiration which used...
ABSTRACT In Escherichia coli the genes encoding anaerobic fumarate respiratory system are transcriptionally regulated by C 4 -dicarboxylates. The regulation is effected a two-component regulatory system, DcuSR, consisting of sensory histidine kinase (DcuS) and response regulator (DcuR). DcuS DcuR encoded dcuSR (previously yjdHG ) at 93.7 min on calculated E. map. Inactivation dcuR dcuS caused loss -dicarboxylate-stimulated synthesis reductase ( frdABCD genes) fumarate-succinate antiporter...
Summary The promoter region and transcriptional regulation of the nuoA‐N gene locus encoding proton‐translocating NADH:quinone oxidoreductase was analysed. A 560 bp intergenic upstream nuo followed by a (designated irhA for L ys R h omo‐logue ) coding regulator similar to those LysR family. Disruption did not affect growth (respiratory or non‐respiratory) expression significantly. Transcriptional electron acceptors, donors regulators ArcA, FNR, NarL NarP, IHF (integration host factor)...
The Gram-positive endospore-forming bacterium Bacillus licheniformis can be found widely in nature and it is exploited industrial processes for the manufacturing of antibiotics, specialty chemicals, enzymes. Both its varied natural habitats settings, B. cells will exposed to increases external osmolarity, conditions that trigger water efflux, impair turgor, cause cessation growth, negatively affect productivity cell factories biotechnological processes. We have taken here both systems-wide...
Abstract Background The production of enzymes by an industrial strain requires a complex adaption the bacterial metabolism to conditions within fermenter. Regulatory events process result in dynamic change transcriptional activity genome. This network genes is orchestrated proteins as well regulatory RNA elements. Here we present RNA-Seq based study considering selected phases industry-oriented fermentation Bacillus licheniformis . Results A detailed analysis 20 strand-specific datasets...
A new binding‐protein‐dependent transport system of Escherichia coli specific for l ‐arginine was characterized by genetic and biochemical means. The is encoded five adjacent genes, artPIQMJ (art standing ar ginine t ransport), which are organized in two transcriptional units ( artPIQM artJ ). artl gene products (Artl ArtJ) periplasmic binding proteins with sequence similarity to polar (basic) amino acids. artQ, artM artP similar the transmembraneous ATPase carriers. mature Artl J were...
Bacillus pumilus is characterized by a higher oxidative stress resistance than other comparable industrially relevant Bacilli such as B. subtilis or licheniformis. In this study the response of to was investigated during treatment with high concentrations hydrogen peroxide at proteome, transcriptome and metabolome level. Genes/proteins belonging regulons, which are known have important functions in organisms, were found be upregulated, Fur, Spx, SOS CtsR regulon. Strikingly, parts...
A novel fed-batch approach for the production of L-phenylalanine (L-Phe) with recombinant E. coli is presented concerning on-line control key fermentation parameters glucose and tyrosine. Two different strains possessing either tyrosine feedback resistant aroF(fbr) (encoding DAHP-synthase (3-desoxy-D-arabino-heptusonate-7-phosphate)) or wild-type aroF(wt) were used as model systems to elucidate necessity finding an individual process optimum each genotype. With aid control, could be L-Phe...
Aminoacylases are highly promising enzymes for the green synthesis of acyl-amino acids, potentially replacing environmentally harmful Schotten-Baumann reaction. Long-chain acids can serve as strong surfactants and emulsifiers, with application in cosmetic industries. Heterologous expression these enzymes, however, is often hampered, limiting their use industrial processes.We identified a novel mycobacterial aminoacylase gene from Mycolicibacterium smegmatis MKD 8, cloned expressed it...
The aim of the present study was characterisation three true subtilisins and one phylogenetically intermediate subtilisin from halotolerant halophilic microorganisms. Considering currently growing enzyme market for efficient novel biocatalysts, data mining is a promising source novel, as yet uncharacterised enzymes, especially or Bacillaceae, which offer great potential to meet industrial needs. Both bacteria Pontibacillus marinus DSM 16465T Alkalibacillus haloalkaliphilus 5271T both...
Tyrosine feedback-inhibits the 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthase isoenzyme AroF of Escherichia coli. Here we show that an Asn-8 to Lys-8 substitution in leads a tyrosine-insensitive DAHP synthase. This mutant enzyme exhibited similar activities (v=30–40 U mg−1) and substrate affinities (Kmerythrose-4-phosphate=0.5 mM, positive cooperativity with respect phospho(enol)pyruvate) as wild-type AroF, but showed decreased thermostability. An engineered lacking seven...