A5074399278- Enzyme Production and Characterization
- Enzyme Structure and Function
- Biofuel production and bioconversion
- Advanced Proteomics Techniques and Applications
- Protein purification and stability
- Protein Hydrolysis and Bioactive Peptides
- Phytase and its Applications
- Microbial Metabolites in Food Biotechnology
- Peptidase Inhibition and Analysis
FH Aachen
2022-2025
The aim of the present study was characterisation three true subtilisins and one phylogenetically intermediate subtilisin from halotolerant halophilic microorganisms. Considering currently growing enzyme market for efficient novel biocatalysts, data mining is a promising source novel, as yet uncharacterised enzymes, especially or Bacillaceae, which offer great potential to meet industrial needs. Both bacteria Pontibacillus marinus DSM 16465T Alkalibacillus haloalkaliphilus 5271T both...
Halophilic and halotolerant microorganisms represent a promising source of salt-tolerant enzymes suitable for various biotechnological applications where high salt concentrations would otherwise limit enzymatic activity. Considering the current growing enzyme market need more efficient new biocatalysts, present study aimed at characterization high-alkaline subtilisin from Alkalihalobacillus okhensis Kh10-101T . The protease gene was cloned expressed in Bacillus subtilis DB104. recombinant...
The subtilase family (S8), a member of the clan SB serine proteases are ubiquitous in all kingdoms life and fulfil different physiological functions. Subtilases divided several groups especially subtilisins interest as they used various industrial sectors. Therefore, we searched for new subtilisin sequences Bacillaceae using data mining approach. obtained 1,400 were phylogenetically classified context family. This required an updated comprehensive overview within this To fill gap, conducted...
Subtilisins from microbial sources, especially the Bacillaceae family, are of particular interest for biotechnological applications and serve currently growing enzyme market as efficient novel biocatalysts. Biotechnological include use in detergents, cosmetics, leather processing, wastewater treatment pharmaceuticals. To identify a possible candidate market, here we cloned gene subtilisin SPFA Fictibacillus arsenicus DSM 15822T (obtained through data mining-based search) expressed it...