A5033504648- Platelet Disorders and Treatments
- Blood transfusion and management
- Blood groups and transfusion
- Microfluidic and Bio-sensing Technologies
- Nanoparticle-Based Drug Delivery
- Blood donation and transfusion practices
- Heparin-Induced Thrombocytopenia and Thrombosis
- Blood properties and coagulation
- Characterization and Applications of Magnetic Nanoparticles
- Venous Thromboembolism Diagnosis and Management
- Glycosylation and Glycoproteins Research
- Cellular Mechanics and Interactions
- Diabetes Treatment and Management
- Antiplatelet Therapy and Cardiovascular Diseases
- SARS-CoV-2 and COVID-19 Research
- Diabetes Management and Research
- Chronic Disease Management Strategies
- Advanced biosensing and bioanalysis techniques
- Angiogenesis and VEGF in Cancer
- Diabetes and associated disorders
- RNA Interference and Gene Delivery
- Acute Ischemic Stroke Management
- Hematopoietic Stem Cell Transplantation
- Inflammatory Biomarkers in Disease Prognosis
- Diabetes Management and Education
Universitätsmedizin Greifswald
2016-2025
Universität Greifswald
2004-2025
Institute of Immunology
2021
Institute for Transfusion Medicine
2020
Friedrich Schiller University Jena
2006
Abstract In life sciences, the material properties of suspended cells have attained significance close to that fluorescent markers but with advantage label-free and unbiased sample characterization. Until recently, cell rheological measurements were either limited by acquisition throughput, excessive post processing, or low-throughput real-time analysis. Real-time deformability cytometry expanded application mechanical assays fast on-the-fly phenotyping large sizes, has been restricted...
The supply of blood products such as red cells poses a challenge due to rising demand and declining donor numbers. Careful matching different types is required. Only type O the A, B, AB can be received by any patient without transfusion incompatibilities. Therefore, O-type considered "universal blood" especially needed in emergency situations. In this study, we focused on conversion B antigen enzymatic deglycosylation generate H determining blood. For this, characterized several previously...
Abstract Background SARS-CoV-2 vaccine ChAdOx1 nCov-19 rarely causes vaccine-induced immune thrombotic thrombocytopenia (VITT) that—like autoimmune heparin-induced thrombocytopenia—is mediated by platelet-activating anti-platelet factor 4 (PF4) antibodies. Methods We investigated vaccine, PF4, and VITT patient-derived anti-PF4 antibody interactions using dynamic light scattering, 3D-super-resolution microscopy, electron microscopy. Mass spectrometry was used to analyze composition. the...
Platelet transfusion has become essential therapy in modern medicine. Although the clinical advantage of platelet been well established, adverse reactions upon transfusion, especially transmission bacterial infection, still represent a major challenge. While contamination is favored by storage platelets at room temperature, cold may solution for this important issue. In study, we aimed to clarify whether plasma protective or detrimental effects on cold-stored platelets. We investigated...
Platelets within one individual display heterogeneity in reactivity, size, age, and expression of surface receptors. To investigate the combined intraindividual contribution platelet receptor levels on reactivity platelets, we studied fractions large small platelets from healthy donors separated by using differential centrifugation. Size-separated were perfused over a collagen-coated to assess thrombus formation. Multicolor flow cytometry was used characterize resting stimulated...
Background A significant number of platelet concentrates (PCs) is discarded daily in blood banks due to limited shelf life. Human lysate (HPL), derived from expired PCs, has gained attention as an ethical and sustainable cell culture media supplement biomedical research therapy production. However, HPL subject decisive disadvantages such batch differences lack storage stability. To overcome these limitations enhance the applicability HPL, we developed manufacturing protocol including a...
SARS-CoV-2 serosurveillance is important to adapt infection control measures and estimate the degree of underreporting. Blood donor samples can be used as a proxy for healthy adult population. In repeated cross-sectional study from April 2020 2021, September April/May 2022, 13 blood establishments collected 134,510 anonymised specimens donors in 28 regions across Germany. These were tested antibodies against spike protein nucleocapsid, including neutralising capacity. Seroprevalence was...
The interaction between human eotaxin (hEotaxin) and its polyclonal antibody anti-human (anti-hEotaxin) was investigated by means of a novel liquid-phase immunoassay using the magneto-optical relaxation ferrofluids. binding quality as well kinetic properties partners determined specifically magnetic probes. For this purpose, nanoparticles (MNP; DDM128N, Meito Sangyo, Japan) were initially functionalized with streptavidin. biotin-nylated conjugated streptavidin-MNP applying...
Background The THERAFLEX UV ‐Platelets pathogen reduction system for platelet concentrates ( PC s) operates with ultraviolet C light UVC ; 254 nm) only without addition of photosensitizers. This phase I study evaluated safety and tolerability autologous ‐irradiated s in healthy volunteers. Methods Eleven volunteers underwent two single (series 1 2) one double apheresis 3). were treated , stored 48 h retransfused a dose‐escalation scheme: 12·5, 25% 50% 1); complete 2); Platelet counts,...
Platelets are the smallest blood cells and important for hemostasis. Platelet concentrates (PC) medicinal products transfused to prevent or treat bleeding. Typically, platelets in PCs assessed by vitro tests their function. However, vivo testing of these is highly desirable. To distinguish from patients probands own after PC transfusions within scope clinical studies, need be efficiently labeled with minimal preactivation prior transfusion. Here we report on a method improved cell uptake...
Real-time deformability cytometry is a unique tool for quality assessment of therapeutic blood cells utilizing their mechanical properties.
Magnetic nanoparticles have recently shown great potential in nonradioactive labeling of platelets. Platelet efficiency is enhanced when particles are conjugated with proteins like human serum albumin (HSA). However, the optimal HSA density coated on and uptake mechanism single platelets remain unclear. Here, we utilized single-molecule force spectroscopy (SMFS) other complementary methods to characterize interaction interacting determine amount required coat particles. An concentration...
Measurements of the magneto-optical relaxation ferrofluids (MORFF) were applied as a novel homogeneous immunoassay for investigation biomolecular interactions. The technique is based on magnetic nanoparticles (MNP) functionalized with antibodies. time optical birefringence that occurs when pulsed field to nanoparticle suspension depends particle size. This enables detection aggregates formed after addition antigen coupling partner. MORFF size measurements original ferrofluid and its...
In the light of ongoing SARS-CoV-2 pandemic, convalescent plasma is a treatment option for CO-VID-19. contrast to usual therapeutic plasma, agents do not represent clotting factor activities, but immunoglobulins. Quarantine storage as measure reduce risk pathogen transmission feasible. Therefore, inactivation (e.g., Theraflex®-MB, Macopharma, Mouvaux, France) an attractive option. Data on impact by methylene blue (MB) antibody integrity are sparse.Antigen-specific binding capacity was tested...
Abstract Cold storage of platelet concentrates (PC) has become attractive due to the reduced risk bacterial proliferation, but in vivo circulation time cold-stored platelets is reduced. Ca 2+ release from organelles and higher activity pumps at temperatures < 15 °C triggers cytoskeleton changes. This suppressed by Mg addition, avoiding a shift hemostasis cytoskeletal alterations. We report on impact 2–10 mM alterations PC stored room temperature (RT) or 4 additive solution (PAS), 30%...
<b><i>Introduction:</i></b> Before being implemented in daily clinical routine, new production strategies for platelet concentrates (PCs) must be validated their efficacy. Besides vitro testing, the establishment of methods requires labeling platelets vivo studies platelets’ survival and recovery. Indocyanine green (ICG) is a Food Drug Administration-approved near-infrared (NIR) fluorescent dye diagnostic use vivo, suitable non-radioactive direct cell platelets....
Abstract Background Structural and biochemical changes in stored platelets are influenced by collection processing methods. This international study investigates the effects of platelet (PLT) storage conditions on HMGB1, sCD40L, sCD62P protein levels concentrate supernatants (PCs). Study Design/Methods PC ( n = 3748) were collected each centre using identical centrifugation methods 9) tested centrally ELISA/Luminex platform. Apheresis versus buffy coat (BC‐PC) method, plasma PAS RT cold...
Magneto-optical relaxation measurements of magnetically labelled biomolecules are a promising tool for immunometric analyses. Carcinoembryonic antigen (CEA) and its polyclonal monoclonal antibodies (anti-CEA) were utilized as model system affinity analysis the interaction between antibody antigen. For this purpose coupled with magnetic nanoparticles (MNPs). Aggregation these sensors due to interactions CEA was observed subsequently by measuring time birefringence transmitted laser beam that...