A5034629546- Blood transfusion and management
- Infective Endocarditis Diagnosis and Management
- Streptococcal Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
- Blood groups and transfusion
- Hepatitis Viruses Studies and Epidemiology
- Bacterial Identification and Susceptibility Testing
- COVID-19 Clinical Research Studies
- Liver Disease and Transplantation
- Blood donation and transfusion practices
- Viral gastroenteritis research and epidemiology
- Antimicrobial Resistance in Staphylococcus
- Hepatitis B Virus Studies
- SARS-CoV-2 detection and testing
- COVID-19 epidemiological studies
- Platelet Disorders and Treatments
- Mosquito-borne diseases and control
- Clostridium difficile and Clostridium perfringens research
- Parvovirus B19 Infection Studies
- Rabies epidemiology and control
- Hemostasis and retained surgical items
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Dermatological and COVID-19 studies
- Hematopoietic Stem Cell Transplantation
- Insect symbiosis and bacterial influences
Heart and Diabetes Center North Rhine-Westphalia
2016-2025
LWL-Universitätsklinikum Bochum
2014-2024
University Hospitals of the Ruhr-University of Bochum
2014-2024
Bielefeld University
2024
Ruhr University Bochum
2008-2023
Institute for Transfusion Medicine
2021
GTx (United States)
2018
The risk of transfusion-transmitted hepatitis E virus (HEV) infections by contaminated blood products remains unknown. In the present study, we evaluated and compared different nucleic acid amplification technique (NAT) methods for detection HEV in components. Minipools a total 16,125 individual donors were screened presence RNA using highly sensitive RealStar RT-PCR kit, revealing minimum limit 4.66 IU/ml. Thirteen positive (0.08%), these donors, only three already showed reactive IgM...
Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2, a new member of the genus Betacoronavirus, is pandemic virus, which has caused numerous fatalities, particularly in elderly and persons with underlying morbidities. At present, there are no approved vaccines nor antiviral therapies available. The detection quantification SARS-CoV-2-neutralizing antibodies plays crucial role assessment immune status convalescent COVID-19 patients, evaluation recombinant therapeutic antibodies,...
Most cases of coronavirus disease 2019 are mild or asymptomatic. Therefore, many remain unrecorded. We determined seroprevalence IgG antibodies against severe acute respiratory syndrome 2 (SARS-CoV-2) in 3,186 regular blood donors three German federal states between 9 March and 3 June 2020. The was 0.91% (95% confidence interval (CI): 0.58-1.24) overall, ranging from 0.66% CI: 0.13-1.19) Hesse to 1.22% 0.33-2.10) Lower-Saxony.
The risk and importance of transfusion-transmitted hepatitis E virus (TT-HEV) infections by contaminated blood products is currently a controversial discussed topic in transfusion medicine. infectious dose, particular, remains an unknown quantity. In the present study, we illuminate review this aspect seen from viewpoint donation service with more than two years experience routine HEV donor screening. We systematically actual status presently known cases TT-HEV available NAT-screening...
In the present study, a novel broad-range real-time PCR was developed for rapid detection of human pathogenic fungi. The assay targets part 28S large-subunit ribosomal RNA (rDNA) gene. We investigated its application most important fungal genera, including Aspergillus, Candida, Cryptococcus, Mucor, Penicillium, Pichia, Microsporum, Trichophyton, and Scopulariopsis. Species were identified in PCR-positive reactions by direct DNA sequencing. A noncompetitive internal control applied to prevent...
Abstract Background A broad spectrum of pathogens is causative for respiratory tract infections, but symptoms are mostly similar. Therefore, the identification viruses and bacteria only feasible using multiplex PCR or several monoplex tests in parallel. Methods The analytical sensitivity three assays, RespiFinder-19, RespiFinder-SMART-22 xTAG-Respiratory-Virus-Panel-Fast-Assay (RVP), were compared to real-time with quantified standardized control material. All assays include most common...
Diagnosis of hepatitis E virus (HEV) is usually determined serologically by detection the presence immunoglobulin (Ig)M antibodies or rising anti-HEV IgG titers. However, serological assays have demonstrated a significant variation in their sensitivities and specificities. In this study, we present systematic comparison different immunological using complete seroconversion panels 10 virologically confirmed HEV genotype 3 infected individuals. Assay were further evaluated testing serially...
Hepatitis E virus (HEV) infection is recognized as an emerging and often undiagnosed disease in industrialized countries, with asymptomatic infections actually occurring blood donors. Sensitive detection of HEV-RNA crucial for diagnosis monitoring progression. We evaluated the analytical sensitivity performance three HEV RT-PCR assays (RealStar reverse transcription-PCR [RT-PCR], hepatitis@ceeramTools, ampliCube RT-PCR) screening individuals (ID-nucleic acid amplification technology...
Asymptomatic hepatitis E virus (HEV) infections have been found in blood donors from various European countries, but the natural course is rarely specified. Here, we compared progression of HEV viraemia, serostatus and liver-specific enzymes 10 with clinically asymptomatic genotype 3 infection, measuring RNA concentrations, plasma concentrations alanine/aspartate aminotransferase, glutamate dehydrogenase bilirubin anti-HEV IgA, IgM IgG antibodies. ranged 77.2 to 2.19×10(5) IU/mL, viraemia...
ObjectivesSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the pulmonary disease 2019 (COVID-19, which has challenged health care facilities worldwide. The sustainability of systems is largely reliant on status their workers (HCW).This study aimed to detect SARS-CoV-2 virus and specific antibodies among HCWs in a German hospital as model system for potential spread pandemic.MethodsBetween March June 2020, we used combination RT-PCR testing RNA an enzyme-linked...
Bacterial contamination is the major infectious hazard associated with transfusion of platelet preparations (PLTs). Routine testing for bacterial in PLTs has become common, but transfusion-transmitted sepsis not been eliminated. Here, we describe a novel flow cytometry-based method point-of-issue screening contamination.We used BactiFlow cytometer to detect and count bacteria based on esterase activity viable cells. We compared assay incubation (BacT/Alert culture system) rapid nucleic...
AimsA new diagnostic strategy to improve the detection of pathogens in heart valves (HVs) from patients with infective endocarditis (IE) was evaluated.
Abstract Background Streptococcus gallolyticus subsp. is an important causative agent of infective endocarditis (IE) but the knowledge on virulence factors limited and pathogenesis infection poorly understood. In present study, we established experimental in vitro IE cell culture model using EA.hy926 HUVEC cells to investigate adhesion invasion characteristics 23 strains from different origins (human IE-derived isolates, other human clinical animal isolates). Adhesion eight components...
Abstract Background Streptococcus gallolyticus subsp. is an important causative agent of infectious endocarditis, while the pathogenicity this species widely unclear. To gain insight into pathomechanisms and underlying genetic elements for lateral gene transfer, we sequenced entire genome pathogen. Results We whole S. strain ATCC BAA-2069, consisting a 2,356,444 bp circular DNA molecule with G+C-content 37.65% novel 20,765 plasmid designated as pSGG1. Bioinformatic analysis predicted 2,309...
Streptococcus gallolyticus subsp. (S. gallolyticus), a member of group D streptococci, is an inhabitant the animal and human gastrointestinal tract. Furthermore, it facultative pathogen which causes e.g. endocarditis, septicemia mastitis. S. may be transmitted either directly or indirectly between animals humans. However, transmission routes are unsolved issue. In this study, we present systematic analyses isolate infective endocarditis patient in relation to isolates his laying hen flock....
Abstract Background and Objectives Platelet concentrates (PC) are stored at 20–24°C to maintain platelet functionality, which may promote growth of contaminant bacteria. Alternatively, cold storage PC limits bacterial growth; however, data related proliferation psychotrophic species in cold‐stored (CSP) scarce, is addressed this study. Materials Methods Eight laboratories participated study with a pool/split approach. Two split units were spiked ~25 colony forming (CFU)/PC Staphylococcus...
Bacterial contamination of platelet concentrates (PCs) still represents an ongoing risk in transfusion-transmitted sepsis. Recently the Pan Genera Detection (PGD) system was developed and FDA licensed for screening bacterial PCs directly prior to transfusion. The test principle is based on immunological detection lipopolysaccharide (for Gram-negative bacteria) or lipoteichoic acid Gram-positive bacteria). In present study we analyzed applicability this method with regard limit,...
Aims Inflammation in infective endocarditis (IE) is a complex network including interactions of inflammatory cytokines and other components host response. Certainly, any variation this could influence susceptibility or disease progression IE. In study, 14 single nucleotide variants (SNVs) genes coding for interleukin-1β, interleukin-6, interleukin-10, toll–like receptor-4, tumor necrosis factor-α, selectin E intercellular adhesion molecule-1 were analyzed an association with to IE correlated...
A high number of SARS-CoV-2 infections are mild, often even asymptomatic. Because specificity and sensitivity, RT-PCR is considered the gold-standard for COVID-19 testing. The technology played a key role in detecting sources infection at an early stage therefore preventing larger outbreaks. This was especially important case critical infrastructure, such as hospitals. Until now, comprehensive studies concerning impact high-frequency PCR-testing German tertiary care hospitals during pandemic...