A5027139291- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Blood transfusion and management
- Travel-related health issues
- Atomic and Subatomic Physics Research
- Escherichia coli research studies
- Lipid Membrane Structure and Behavior
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Machine Learning in Bioinformatics
- Photosynthetic Processes and Mechanisms
- Malaria Research and Control
- Mosquito-borne diseases and control
- Genomics and Phylogenetic Studies
- Gene expression and cancer classification
- Bacillus and Francisella bacterial research
- Blood donation and transfusion practices
- Hemostasis and retained surgical items
- Protein Structure and Dynamics
DRK-Blutspendedienst Baden-Württemberg - Hessen
2024
German Red Cross
2024
University of Tübingen
2014-2023
Bacterial type III protein secretion systems inject effector proteins into eukaryotic host cells in order to promote survival and colonization of Gram-negative pathogens symbionts. Secretion across the bacterial cell envelope injection is facilitated by a so-called injectisome. Its small hydrophobic export apparatus components SpaP SpaR were shown nucleate assembly needle complex form central "cup" substructure Salmonella Typhimurium system. However, vivo placement these their function...
Precisely knowing the stoichiometry of their components is critical for investigating structure, assembly, and function macromolecular machines. This has remained a technical challenge in particular large, hydrophobic membrane-spanning protein complexes. Here, we determined type III secretion system Salmonella enterica serovar Typhimurium using two complementary protocols gentle complex purification combined with peptide concatenated standard synthetic stable isotope-labeled peptide-based...
Abstract Background and Objectives Platelet concentrates (PC) are stored at 20–24°C to maintain platelet functionality, which may promote growth of contaminant bacteria. Alternatively, cold storage PC limits bacterial growth; however, data related proliferation psychotrophic species in cold‐stored (CSP) scarce, is addressed this study. Materials Methods Eight laboratories participated study with a pool/split approach. Two split units were spiked ~25 colony forming (CFU)/PC Staphylococcus...
Abstract Background and Objectives Plasmodium species are naturally transmitted by Anopheles mosquitos. The parasite infects red blood cells (RBCs) can be transfused with products. In non‐endemic areas, the main risk of infection arises from travellers coming back people immigrating malaria‐endemic regions. Endemic countries face a permanent transfusion‐transmitted malaria (TTM). TTM may cause life‐threatening complications in patients dependent on donations. This study aimed to investigate...
The reliable detection of protein-protein interactions by affinity purification mass spectrometry (AP-MS) is crucial for the understanding biological processes. Quantitative information can be used to separate truly interacting proteins from false-positives contrasting counts binding specific baits with negative controls. Several approaches have been proposed computing scores potential interaction proteins, example, commonly SAINT software. However, it remains a subjective decision where set...