A5061287181- RNA modifications and cancer
- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Systemic Lupus Erythematosus Research
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- interferon and immune responses
- Inflammatory Bowel Disease
- Ferroptosis and cancer prognosis
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Microscopic Colitis
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Genomics, phytochemicals, and oxidative stress
- Cancer, Lipids, and Metabolism
- Protein Degradation and Inhibitors
- Peptidase Inhibition and Analysis
- Epigenetics and DNA Methylation
Guangzhou Institutes of Biomedicine and Health
2008-2025
University of Hong Kong
2021-2025
Shanghai First Maternity and Infant Hospital
2020-2025
Guangzhou Medical University
2020-2024
Chinese Academy of Sciences
2008-2024
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2020-2024
Shaanxi University of Chinese Medicine
2017-2024
Jiangxi University of Traditional Chinese Medicine
2024
University of Chinese Academy of Sciences
2020-2023
New York University
2022
The genetic program of embryonic stem (ES) cells is orchestrated by a core transcription factors that has OCT4, SOX2, and NANOG as master regulators. Protein levels these are tightly controlled autoregulatory feed-forward transcriptional mechanisms in order to prevent early differentiation. Recent studies have shown knockdown Esrrb (estrogen-related-receptor β), member the nuclear orphan receptor family, induces differentiation mouse ES cultured presence leukemia inhibitory factor. It was...
Purinergic ionotropic P2X7 receptors (P2X7Rs) are closely associated with excitotoxicity and nociception. Inhibition of P2X7R activation has been considered as a potentially useful strategy to improve recovery from spinal cord injury reduce inflammatory damage trauma. The physiological functions P2X7Rs, however, poorly understood, even though such information is essential for making the an effective therapeutic target. We show here that P2X7Rs in satellite cells dorsal root ganglia tonically...
Known as a histone H3K9 methyltransferase, SETDB1 is essential for embryonic development and pluripotent inner cell mass (ICM) establishment. However, its function in pluripotency regulation remains elusive. In this study, we find that under the "ground state" of with two inhibitors (2i) MEK GSK3 pathways, Setdb1-knockout fails to induce trophectoderm (TE) differentiation serum/LIF (SL), indicating TE fate restriction not direct target SETDB1. both conditions, activates group genes targeted...
Fruit color is an important horticultural trait, which greatly affects consumer preferences. In tomato, fruit determined by the accumulation of different pigments, such as carotenoids in pericarp and flavonoids peel, along with degradation chlorophyll during ripening. Since a multigenic it takes years to introgress all color-related genes single genetic background via traditional crossbreeding, avoidance linkage drag this process difficult. Here, we proposed rapid breeding strategy generate...
Abstract Cellular senescence is characterized by a decrease in protein synthesis, although the underlying processes are mostly unclear. Chemical modifications to transfer RNAs (tRNAs) frequently influence tRNA activity, which crucial for translation. We describe how N7-methylguanosine (m7G46) methylation, catalyzed METTL1-WDR4, regulates translation and influences phenotypes. Mettl1/Wdr4 m7G gradually diminish with aging. A METTL1 causes reduction tRNAs, especially those modification, via...
A hypoxic microenvironment is a common feature of skin wounds. Our previous study demonstrated that three-dimensional coculture umbilical cord-derived mesenchymal stem cells (ucMSCs) and endothelial facilitates cell communication host integration in tissue engineering. Here, we aimed to identify the mechanism by which ucMSCs affect under conditions after injury. We demonstrate hypoxia enhances exosome-mediated paracrine function ucMSCs, increases proliferation migration. In mouse...
Dysregulation of Notch signaling has been implicated in cellular transformation and tumorigenesis a variety cancers while potential roles MIB1, an E3 ubiquitin ligase required for efficient activation, remains to be investigated. We analyzed MIB1 expression levels tumor samples performed gain-of-function loss-of-function studies cell lines investigate epithelial-to-mesenchymal transition (EMT), migration, survival. found that overexpression is detected subset lung squamous carcinoma...
Traumatic hemorrhagic shock (THS) is a major cause of mortality and morbidity worldwide in severely injured patients. Mesenchymal stem cells (MSCs) possess immunomodulatory properties tissue repair potential mainly through paracrine pathway mediated by MSC-derived extracellular vesicles (MSC-EVs). Interleukin 10 (IL-10) potent anti-inflammatory cytokine that plays crucial role during the inflammatory response, with broad range effects on innate adaptive immunity, preventing damage to host...
Cell fate decision involves rewiring of the genome, but remains poorly understood at chromatin level. Here, we report that remodeling complex NuRD participates in closing open early phase somatic reprogramming. Sall4, Jdp2, Glis1 and Esrrb can reprogram MEFs to iPSCs efficiently, only Sall4 is indispensable capable recruiting endogenous components NuRD. Yet knocking down reduces reprogramming modestly, contrast disrupting known Sall4-NuRD interaction by mutating or deleting interacting motif...
The ciliary hypothesis for cystic renal diseases postulates that most of these conditions result from abnormalities in the primary cilium, a microtubule-based structure acts as sensor extracellular cues. Inactivation von Hippel-Lindau (VHL) tumor suppressor gene predisposes to cysts and clear cell carcinoma. VHL plays critical role formation cilia kidney epithelium, but underlying mechanisms are poorly understood. Here, we demonstrate inactivation induces HEF1/Cas-L/NEDD9 Aurora kinase A via...
Abstract Some transcription factors that specifically bind double-stranded DNA appear to also function as RNA-binding proteins. Here, we demonstrate the factor Sox2 is able directly RNA in vitro well mouse and human cells. targets via a 60-amino-acid binding motif (RBM) positioned C-terminally of high mobility group (HMG) box. can associate with simultaneously form ternary RNA/Sox2/DNA complexes. Deletion RBM does not affect selection target genes but mitigates pluripotency related...
Abstract STUDY QUESTION Is the sperm acrosome membrane-associated protein 1 (SPACA1) gene critical to human globozoospermia? SUMMARY ANSWER The biallelic loss-of-function (variant of SPACA1) causes globozoospermia as a result acrosome–acroplaxome complex damage. WHAT IS KNOWN ALREADY SPACA1 expression decreases in patients with globozoospermia. Spaca1 gene-disrupted mice have abnormally shaped heads that resemble those DESIGN, SIZE, DURATION We recruited consanguineous family two brothers...
Abstract The sorting of soluble secretory proteins from the endoplasmic reticulum (ER) to Golgi complex is mediated by coat protein II (COPII) vesicles and thought required specific ER membrane cargo-receptor proteins. However, these receptors remain largely unknown. Herein, we show that transfer vesicular cargo requires WFS1, an ER-associated whose loss function leads Wolfram syndrome. Mechanistically, WFS1 directly binds including proinsulin via its luminal C-terminal segment, whereas...
Membraneless condensates, such as stress granules (SGs) and processing bodies (P-bodies), have attracted wide attention due to their unique feature of rapid response without first requiring nuclear feedback. In this study, we identify diaphanous-related formin 3 (DIAPH3), an actin nucleator, a scaffold protein initiate liquid-liquid phase separation (LLPS) form abundant cytosolic phase-separated DIAPH3 (D-granules) in mammalian cells HeLa, HEK293, fibroblasts under various conditions....
Mass spectrometry (MS)-based proteomics has become the most powerful approach to study proteome of given biological and clinical samples. Advancements in sample preparation MS detection have extended application but also brought new demands on data analysis. Appropriate analysis workflow mainly requires quality control, hypothesis testing, functional mining, visualization. Although there are numerous tools for each process, an efficient universal tandem toolkit obtain a quick overall view...
Abstract Cell fate determination at the chromatin level is not fully comprehended. Here, we report that c-JUN acts on loci to limit mesoderm cell specification as cells exit pluripotency. Although widely expressed across various types in early embryogenesis, it essential for maintaining Instead, functions a repressor constrain development while having negligible impact ectoderm differentiation. interacts with MBD3–NuRD complex, which helps maintain low accessibility state mesoderm-related...
Background Hypoxia is associated with the evasion of triple-negative breast cancer (TNBC) from immune surveillance. increases subpopulation putative TNBC stem-like cells (TNBCSCs) through activating Wnt/β-Catenin signaling. The shedding MHC class I-related chain A (MICA) particularly noteworthy in stem (CSCs), promoting resistance CSCs to natural killer (NK) cell cytotoxicity. To reestablish MICA/NKG2D-mediated immunosurveillance, we proposed design a fusion protein (SHH002-hu1-MICA) which...
Inactivation of the transcription factor BCL11B reprograms T cells into induced-T-to-NK (ITNKs). However, it remains unclear how suppresses natural killer (NK) cell transcriptional programs. Here, we identified that DNA methyltransferase DNMT1 physically interacts with BCL11B, increasing stability and fidelity methylation maintenance for NK cell–related genes, thereby repressing their expression. Moreover, maintains epigenetic silencing a distinct subset genes independent BCL11B. inhibition...