A5015544231- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Autophagy in Disease and Therapy
- Extracellular vesicles in disease
- Connexins and lens biology
- Sirtuins and Resveratrol in Medicine
- interferon and immune responses
- Inflammatory mediators and NSAID effects
- Microbial Metabolic Engineering and Bioproduction
- ATP Synthase and ATPases Research
- Intracerebral and Subarachnoid Hemorrhage Research
- Photosynthetic Processes and Mechanisms
- Intraocular Surgery and Lenses
- Peroxisome Proliferator-Activated Receptors
- Gestational Diabetes Research and Management
- Ginkgo biloba and Cashew Applications
- Enzyme Catalysis and Immobilization
- Pregnancy and preeclampsia studies
- Lung Cancer Treatments and Mutations
- Ubiquitin and proteasome pathways
Shanghai University of Medicine and Health Sciences
2019-2025
Jiading District Central Hospital
2020-2025
Shanghai University of Traditional Chinese Medicine
2023-2024
University of Shanghai for Science and Technology
2023-2024
Hangzhou Normal University
2024
Ya'an Polytechnic College
2024
Chengdu University of Traditional Chinese Medicine
2024
Soochow University
2024
Kanion Pharmaceutical (China)
2018-2024
First Affiliated Hospital of Soochow University
2024
Ginkgolide terpenoid lactones, including ginkgolides and bilobalide, are two crucial bioactive constituents of extract Ginkgo biloba (EGb) which was used in the treatment cardiovascular cerebrovascular diseases. The aims this study were to investigate antioxidant effects mechanism (ginkgolide A (GA), ginkgolide B (GB), K (GK)) bilobalide (BB) against oxidative stress induced by transient focal cerebral ischemia. In vitro, SH-SY5Y cells exposed oxygen-glucose deprivation (OGD) for 4 h...
Abstract Cancer stem cells (CSCs) represent a major source of treatment resistance and tumor progression. However, regulation CSCs stemness is not entirely understood. Here, we report that TSPAN8 expression upregulated in breast CSCs, promotes the gene NANOG, OCT4, ALDHA1, correlates with therapeutic resistance. Mechanistically, interacts PTCH1 inhibits degradation SHH/PTCH1 complex through recruitment deubiquitinating enzyme ATXN3. This results translocation SMO to cilia, downstream...
Hypoxia is commonly observed in solid tumors and contributes to the resistance of DNA damage drugs. However, mechanisms behind this are still unclear. In study, we aimed explore effects hypoxia-induced exosomes on non-small cell lung cancer (NSCLC). Methods: NSCLC cells were subjected either normoxic or hypoxic conditions assess survival changes expression levels key proteins. Comparative proteomics performed identify exosomal PKM2 cisplatin-resistant cells-derived exosomes. Functions...
Abstract Dysregulation of the sterol regulatory element-binding transcription factors protein (SREBP) and SREBF activates de novo lipogenesis to high levels in cancer cells, a critical event driving malignant growth. In this study, we identified an important posttranslational mechanism by which SREBP1a is regulated during metabolic reprogramming cells. Mass spectrometry revealed arginine methyltransferase 5 (PRMT5) as binding partner that symmetrically dimethylated it on R321, thereby...
The active ingredient of the traditional Chinese medicine comfrey is shikonin, a naphthoquinone compound. focus this study was to investigate effect shikonin on proliferation, invasion, migration, and chemoresistance non-small cell lung cancer (NSCLC) cells, explore its underlying molecular biological mechanisms. results show that inhibited viability, migration NSCLC cells A549 PC9, induced apoptosis. As inhibitor pyruvate kinase M2 (PKM2), key enzyme in glycolysis, glucose uptake production...
Lipopolysaccharide (LPS)-induced autophagy inhibition in lung fibroblasts is closely associated with the activation of phosphatidylinositol 3-kinase/protein kinase B/mammalian target rapamycin (PI3K-Akt-mTOR) pathway. However, underlying mechanism remains unknown. In this study, we demonstrated that LPS activated PI3K-Akt-mTOR pathway and inhibited fibroblast by depleting thymocyte differentiation antigen-1 (Thy-1) upregulating integrin β3 (Itgb3). Challenge human MRC-5 cell line resulted...
Background Hypoxia is associated with the evasion of triple-negative breast cancer (TNBC) from immune surveillance. increases subpopulation putative TNBC stem-like cells (TNBCSCs) through activating Wnt/β-Catenin signaling. The shedding MHC class I-related chain A (MICA) particularly noteworthy in stem (CSCs), promoting resistance CSCs to natural killer (NK) cell cytotoxicity. To reestablish MICA/NKG2D-mediated immunosurveillance, we proposed design a fusion protein (SHH002-hu1-MICA) which...
Rationale: Non-small cell lung cancer (NSCLC) is a predominant cause of cancer-related mortality, with its progression and treatment resistance significantly influenced by stem cells (CSCs) their complex intercellular communication mechanisms. Small extracellular vesicles (sEVs) have emerged as pivotal mediators signaling, affecting tumor microenvironment modulation therapeutic resistance. This study investigates the role CSC-derived sEVs in transmitting stemness traits through selective...
Acute lymphoblastic leukemia (T-ALL) is one of the most common hematologic malignancies in children worldwide. Despite advances chemotherapy recent years, more than 50% adult T-ALL patients still experience treatment resistance and relapse/refractory disease. Moringa oleifera , a new food resource China, has high nutritional value various pharmacological activities. protein main active ingredients leaves, some studies have shown that antioxidant, anti-inflammatory, antibacterial, anticancer,...
Colorectal cancer (CRC) remains one of the most commonly diagnosed malignancies worldwide. Circular RNAs (circRNAs) are being found to play crucial roles in human cancer, including CRC. The purpose this study was explore function and mechanism circ_0007031 on CRC progression 5-fluorouracil (5-FU) resistance. levels circ_0007031, ATP-binding cassette subfamily C member 5 (ABCC5) miR-133b were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. Cell survival...
Abstract Background Anti-angiogenic therapy has been widely applied to the clinical treatment of malignant tumors. However, efficacy such treatments called into question, especially in triple-negative breast cancer (TNBC). Bevacizumab, first anti-angiogenic agent approved by FDA, actually increases invasive and metastatic properties TNBC cells, resulting from activation Wnt/β-catenin signaling response hypoxia. As a critical receptor signaling, Frizzled-7 (Fzd7) is aberrantly expressed TNBC,...
Owing to their dual-resonance enhanced sensitivity, cavity optomechanical systems provide an ideal platform for ultrasound sensing. In this work, we realize high-sensitivity air-coupled sensing from kilohertz megahertz frequency range based on whispering gallery mode microcavities. Using a $57$-$\ensuremath{\mu}\mathrm{m}$-diameter microtoroid with high optical Q factor (approximately ${10}^{7}$) and mechanical $700$), achieve sensitivities of...
Abstract Abnormal energy metabolism is one of the characteristics tumours. In last few years, more and attention being paid to role regulation tumour aerobic glycolysis. Cancer cells display enhanced glycolysis, also known as Warburg effect, whereby absorb glucose produce a large amount lactic acid under conditions favour proliferation metastasis. this study, we report that haploinsufficient suppressor ASPP2, can inhibit HCC growth stemness by regulating effect through WNT/β‐catenin pathway....
Abstract Protein kinase B (AKT) hyperactivation and de novo lipogenesis are both common in tumor progression. Sterol regulatory element‐binding protein 1 (SREBP1) is the master regulator for lipid metabolism, arginine methyltransferase 5 (PRMT5) an enzyme that can catalyze symmetric dimethyl (SDMA) modification of mature form SREBP1 (mSREBP1) to induce its hyperactivation. Here, we report SDMA‐modified mSREBP1 (mSREBP1‐SDMA) was overexpressed correlated with Ser473‐phosphorylated AKT...
Background: Small extracellular vesicles (sEVs) mediate intercellular communication in the tumor microenvironment (TME) and contribute to malignant transformation of tumors, including unrestricted growth, metastasis, or therapeutic resistance. However, there is a lack agents targeting sEVs overcome reverse chemotherapy resistance through sEVs-mediated TME reprogramming. Methods: The paclitaxel (PTX)-resistant A549T cell line was used explore inhibitory effect alpha-hederin on impeding...
Non-alcoholic fatty liver disease (NAFLD), particularly advanced non-alcoholic steatohepatitis (NASH), leads to irreversible damage. This study investigated the therapeutic effects and potential mechanism of a novel extract from traditional Chinese medicine Alisma orientale (Sam.) Juzep (AE) on free acid (FFA)-induced HepG2 cell model high-fat diet (HFD) + carbon tetrachloride (CCl
More than 50% of new drug candidates in discovery are lipophilic and exhibit poor aqueous solubility, which results bioavailability a lack dose proportionality. Here, we improved the solubility pedunculoside (PE) by generating water-soluble inclusion complex composed PE polymer β-cyclodextrin (CDP). We characterized this novel 1H NMR, FT-IR, UV-vis spectroscopy, powder X-ray diffractometry thermogravimetric analysis. The ratio (β-CD) units CDP to was determined be 2∶1. KD value 4.29×10−3...
Although cancer cells are known to be "addicted" glucose, the effect of glucose in proliferation these remains elusive. Here, we report that upon induction, protein arginine methyltransferase 5 (PRMT5) exerts a profound on G1-S cell cycle progression via directly interacting with cyclin dependent kinase 4 (CDK4) hepatocellular carcinoma (HCC). Upregulation both PRMT5 and CDK4 predicts more malignant characteristics human HCC tissues. Mechanistically, promotes interaction between CDK4, which...
Non-small-cell lung cancer (NSCLC) is one of the deadliest cancers worldwide, and metastasis considered leading causes treatment failure in NSCLC. Wnt/β-catenin signaling crucially involved epithelial-mesenchymal transition (EMT), a crucial factor promoting metastasis, also contributes to resistance developed by NSCLC targeted agents. Frizzled-7 (Fzd7), critical receptor signaling, aberrantly expressed has been confirmed be positively correlated with poor clinical outcomes. SHH002-hu1,...
Epithelial ovarian cancer (EOC) has a very poor prognosis because of tumor invasiveness. Here, we reported that SET and MYND domain containing protein 3 (SMYD3), lysine methyltransferase, was frequently upregulated in EOC associated with prognosis. A series vitro assays demonstrated SMYD3 significantly upgraded the migration ability cells. The results vivo metastasis models further confirmed overexpression promoted progression. Mechanistic investigations indicated cloud decrease p53...
The present study was undertaken to identify whether prostaglandin E2 receptor is the potential receptor/binding site for Ginkgolide A, B, K, and Bilobalide, four main ingredients of Ginkgo biloba L., leaves. Using functional assays, we identified EP4, coupled with Gs protein, as a target B. In human neuroblastoma SH-SY5Y cells suffered from oxygen-glucose deprivation/reperfusion, B-activated PKA, Akt, ERK1/2 well Src-mediated transactivation epidermal growth factor receptor. These resulted...
To determine whether protein kinase C-iota (PKC-iota) is associated with glucose metabolism in non-small-cell lung cancer (NSCLC) and its regulatory effect on metabolic biological changes observed NSCLC can be mediated by transporter 1 (GLUT1).Forty-five patients underwent combined 18F-fludeoxyglucose (18F-FDG) positron emission tomography computed (PET/CT) before surgery, another eighty-one were followed-up for 1-91 months after tumor resection. The rate of was quantified measuring the...