Abstract Background Long non-coding RNAs (lncRNAs) have been associated with non-small cell lung cancer (NSCLC), but the underlying molecular mechanisms of their specific roles in mediating aerobic glycolysis poorly explored. Methods Next-generation RNA sequencing assay was performed to identify differentially expressed between NSCLC tissues high 18 F-fluorodeoxyglucose (FDG) uptake and adjacent normal tissues. LINC01123 expression measured by real-time PCR situ hybridization (ISH) assay....

Rationale: Non-small cell lung cancer (NSCLC) is a deadly disease with hallmark of aberrant metabolism. The mechanism glycolysis associated lncRNA underlying the aggressive behaviors NSCLC poorly understood. Methods: expression level AC020978 in was measured by quantitative real-time PCR and fluorescence situ hybridization (FISH) assay. biological role proliferation aerobic determined functional experiments vitro vivo. transcription assessed dual-luciferase reporter chromatin...

Hypoxia is commonly observed in solid tumors and contributes to the resistance of DNA damage drugs. However, mechanisms behind this are still unclear. In study, we aimed explore effects hypoxia-induced exosomes on non-small cell lung cancer (NSCLC). Methods: NSCLC cells were subjected either normoxic or hypoxic conditions assess survival changes expression levels key proteins. Comparative proteomics performed identify exosomal PKM2 cisplatin-resistant cells-derived exosomes. Functions...

One of the obstacles limiting progress in development effective cancer therapies is shortage preclinical models that capture dynamic nature tumor microenvironments. Interstitial flow strongly impacts response to chemotherapy; however, conventional vitro largely disregard this key feature. Here, a proof principle microfluidic platform for generation large arrays breast spheroids are grown under close-to-physiological biomimetic hydrogel reported. This spheroids-on-a-chip model used time- and...

The region of Saccharomyces cerevisiae chromosome III located between the 5' end HIS4 gene and 3' adjacent BIK1 has a very high level meiotic recombination. In wild-type strains, meiosis-specific double-strand DNA break occurs in hot spot region. This is absent strains which transcription factors Rap1p, Bas1p, Bas2p cannot bind to upstream HIS4. with levels recombination that are higher than those wild type, found at elevated levels. linear relationship activity frequency breaks suggests...

Abstract Treatment of osseous defects remains a formidable clinical challenge. Porous titanium alloys (pTi) have been emerging as ideal endosseous implants due to the excellent biocompatibility and structural properties, whereas inadequate osseointegration poses risks for unreliable long-term implant stability. Substantial evidence indicates that pulsed electromagnetic fields (PEMF), safe noninvasive method, inhibit osteopenia/osteoporosis experimentally clinically. We herein investigated...

Abstract Dysregulation of small nucleolar RNA host gene 6 (SNHG6) exerts critical oncogenic effects and facilitates tumourigenesis in human cancers. However, little information about the expression pattern SNHG6 ovarian clear cell carcinoma (OCCC) is available, contributions this long non‐coding to progression OCCC are unclear. In present study, we showed via quantitative real‐time PCR that was abnormally up‐regulated tissues relative unpaired normal tissues. High correlated with vascular...

The high heterogeneity of colorectal cancer (CRC) is the main clinical challenge for individualized therapies. Molecular classification will contribute to drug discovery and personalized management optimizing. Here, we aimed characterize molecular features CRC by a system based on metabolic gene expression profiles. 435 samples from Genomic Data Commons data portal were chosen as training set while 566 sample in GSE39582 selected testing set. Then, non-negative matrix factorization...

Emerging evidence has demonstrated the potential of circulating tumor DNA (ctDNA) methylation in application cancer diagnosis.Three genes including Septin9, Syndecan-2 (SDC2), and branched-chain amino acid transaminase 1 (BCAT1), which have been well to aberrant expression colorectal (CRC) as suppressors, were selected for detection. A total 234 peripheral plasma samples from 104 patients with CRC 130 polyps, 60 healthy controls, collected before any treatment. real-time polymerase chain...

In a wild-type strain of Saccharomyces cerevisiae, hotspot for meiotic recombination is located upstream the HIS4 gene. An insertion 49-bp telomeric sequence into coding region strongly stimulates and local formation meiosis-specific double-strand DNA breaks (DSBs). When strains are constructed in which both hotspots heterozygous, activity substantially less when on same chromosome than they opposite chromosomes.

Abstract Transcatheter arterial embolization (TAE) is an extensively applied treatment method for hepatocellular carcinoma (HCC). However, the worsened tumor microenvironment (TME, e.g., reduced pH post‐TAE) may result in unsatisfactory therapeutic outcome. Herein, a new kind of embolic agent, calcium carbonate encapsulated alginate microspheres (CaCO 3 ‐ALG MSs) are synthesized. Such CaCO MSs able to neutralize owing reaction with protons, which would not affect overall morphology after...

Abstract Meiotic recombination in Saccharomyces cerevisiae is initiated by double-strand DNA breaks (DSBs). Using two approaches, we mapped the position of DSBs associated with a hotspot created insertion telomeric sequences into region upstream HIS4. We found that have no obvious sequence specificity and localize to ~50 bp adjacent insertion. By mapping studies exonuclease III sensitivity broken ends, conclude most molecules blunt ends 3′-hydroxyl groups.

Neohesperidin, a flavonoid compound found in high amounts Poncirus trifoliata, has free radical scavenging activity. For the first time, our study indicated that neohesperidin also induces cell apoptosis human breast adenocarcinoma MDA-MB-231 cells, which was possibly mediated by regulating P53/Bcl-2/Bax pathway. cells were subjected to treatment with neohesperidin. MTT and Trypan blue exclusion assays applied assess viability. The morphological changes of observed using an inverted...

Abstract Long non–coding RNA AC020978 (lncRNA AC020978) is an oncogenic regulator of non–small cell lung cancer (NSCLC). However, the function in regulating NSCLC metastasis and potential molecular mechanism remains largely unknown. In this study, we evaluated expression levels a series tissues using FISH assays found that higher were closely associated with unfavorable prognosis. Functional studies showed promoted migration invasion both vitro vivo. Further investigation demonstrated...

Metformin, one of the most widely prescribed antihyperglycemic drugs, has recently received increasing attention for its potential effects with regard to cancer prevention and treatment. However, mechanisms behind suppression cell growth by metformin remain far from completely understood. The aim present study was investigate whether could regulate histone modification downstream gene transcription, function in inhibiting breast proliferation. A T47D proliferation curve determined counting...

Breast cancer (BC) is a complex disease with high heterogeneity, which often leads to great differences in treatment results. Current common molecular typing method PAM50, shows positive results for precision medicine; however, room improvement still remains because of the different prognoses subtypes. Therefore, this article, we used lncRNAs, are more tissue-specific and developmental stage-specific than other RNAs, as markers combined single-cell expression profiles retype BC, provide new...

In 2008, we isolated a strain of an Amphobotrys sp., CotAr-12, from cotton plant showing Verticillium wilt symptoms in Hubei Province, China. October 2010 and 2011, surveys for sp. on were conducted 14 counties Hubei. No signs was observed cotton. However, gray mold disease found the weed, Acalypha australis 40 51 fields with average incidence 18.6%. The started inflorescences at top stems or branches then spread downward to main stems, branches, leaves. Abundant sporulation necrotic tissues...

To investigate the expression of Inhibin B between various clinical stages, Chinese medicine dialectic typing, and in nasopharyngeal carcinoma (NPC) tissues serum, to evaluate potential as a new biomarker for NPC. Paraffin specimens pathologically confirmed NPC paracancerous were retrospectively collected, α (INHA) βB (INHBB) was detected by SP method, their relationship with clinicopathological indexes analyzed; addition, patients who had received radiotherapy included study subjects,...

Pure mucinous breast cancer (PMC) is a rare histological type with favourable prognosis. However, cases recurrence have been reported and diagnosed in clinical practice. The mechanisms underlying PMC remain unknown. In this study, we aimed to identify the prognostic factors associated PMC.