A5101665334- Bone and Dental Protein Studies
- Bone Metabolism and Diseases
- Cancer, Hypoxia, and Metabolism
- Bone health and treatments
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Cancer, Lipids, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- S100 Proteins and Annexins
- Cancer-related Molecular Pathways
- Cell Adhesion Molecules Research
- Autophagy in Disease and Therapy
- Mesenchymal stem cell research
- Cell death mechanisms and regulation
- RNA modifications and cancer
- Cancer Mechanisms and Therapy
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Cholesterol and Lipid Metabolism
- Spinal Fractures and Fixation Techniques
- Kruppel-like factors research
- Immune Cell Function and Interaction
- Lipid metabolism and biosynthesis
- Epigenetics and DNA Methylation
People's Liberation Army 401 Hospital
2024-2025
Shanghai University of Traditional Chinese Medicine
2021-2024
Chinese PLA General Hospital
2012-2024
Chinese People's Liberation Army
2024
Shanghai Changzheng Hospital
2024
Jiading District Central Hospital
2022-2023
Shanghai University of Medicine and Health Sciences
2018-2023
Nanjing Agricultural University
2018
Second Military Medical University
2006-2017
Fudan University
2011-2015
Hypoxia is commonly observed in solid tumors and contributes to the resistance of DNA damage drugs. However, mechanisms behind this are still unclear. In study, we aimed explore effects hypoxia-induced exosomes on non-small cell lung cancer (NSCLC). Methods: NSCLC cells were subjected either normoxic or hypoxic conditions assess survival changes expression levels key proteins. Comparative proteomics performed identify exosomal PKM2 cisplatin-resistant cells-derived exosomes. Functions...
Survivin, a human inhibitor of apoptosis protein (IAP), plays an important role in both cell cycle regulation and inhibition apoptosis. Survivin is expressed cells during the G(2)/M phase cycle, followed by rapid decline mRNA levels at G(1) phase. It has been suggested that cycle-dependent expression survivin regulated transcriptional level. In this study we demonstrate involvement ubiquitin-proteasome pathway post-translational survivin. short-lived with half-life about 30 minutes...
Expression of osteopontin correlates with tumor progression and metastasis. The mechanisms by which promotes cell survival remain unclear. Here we used short-hairpin RNA-mediated gene silencing to investigate the antitumor effects depletion in hepatocellular carcinoma (HCC).We applied polyethylenimine nanoparticles deliver a RNA for expression HCC cells. Tumorigenicity metastatic potentials cells were studied vitro nude mice. Nuclear factor-kappaB (NF-kappaB) activation was analyzed gel...
The ankyrin-repeat–containing, SH3-domain–containing, and proline-rich-region–containing protein (ASPP) family of proteins regulates apoptosis through interaction with p53 its members. This study evaluated the epigenetic regulation ASPP1 ASPP2 in hepatitis B virus (HBV)-positive hepatocellular carcinoma (HCC) explores effects down-regulation on development HCC. HCC cell lines tissues from patients were used to examine expression methylation ASPP2. was diminished cells by silence owing...
// Lei Cao 1, 2, 5, * , Xiaoyu Fan Wei Jing 3 Yingchao Liang 2 Rui Chen Yingying Liu Minhui Zhu Rongjie Jia Hao Wang Xueguang Zhang 5 Yanyun 1 Xuyu Zhou Jian Zhao 4, 6 Yajun Guo School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, People's Republic China International Joint Cancer Institute, The Second Military Medical 200433, Changhai Hospital, 4 PLA General Hospital Center, Postgraduate Beijing, 100853, Clinical Immunology Laboratory, First Affiliated Suzhou Suzhou, 215007,...
Ursolic acid (UA), a pentacyclic triterpenoid that has been found in broad variety of fruits, spices and medicinal plants, various biological effects such as reducing inflammation, protecting cells from damage, preserving brain function. However, its impact on ferroptosis cancer stem‑like remains unexplored. The present study investigated the effect UA MDA‑MB‑231 BT‑549 cell‑derived triple‑negative breast CSCs (BCSCs) potential pathway. BCSCs were demonstrated by detection...
Abstract Tumor metastasis leads to high mortality; therefore, understanding the mechanisms that underlie tumor is crucial. Generally seen as a secretory protein, osteopontin (OPN) involved in multifarious pathophysiological events. Here, we present novel pro-metastatic role of OPN during metastatic colonization. Unlike (sOPN), which triggers epithelial–mesenchymal transition (EMT) initiate cancer metastasis, intracellular/nuclear (iOPN) induces mesenchymal–epithelial (MET) facilitate...
To test the effects of a novel monoclonal antibody (mAb) against human osteopontin (OPN) in prevention and treatment collagen-induced arthritis (CIA) to elucidate underlying mechanisms these effects.DBA/1J mice immunized with type II collagen induce CIA were monitored assess anti-OPN mAb on clinical severity disease, pathologic changes joints examined histologically. The survival activated T cells from arthritic synovial fluid patients rheumatoid (RA) determined by TUNEL assay or annexin V...
Lipid reprogramming has been considered as a crucial characteristic in hepatocellular carcinoma (HCC) initiation and progression. However, detailed molecular mechanisms have yet to be clearly defined. Here, we examined the effects of tumor suppressor TIP30 on regulation HCC lipid metabolism. We found that decreased expression leads elevated fatty acid synthesis enhanced levels lipogenic enzymes SCD FASN cells. Moreover, SREBP1 is one key transcription factors regulating liver metabolism,...
Neutrophil extracellular traps (NETs) has been demonstrated to regulate the metastasis of breast cancer. In this study, we showed that de novo cholesterol biosynthesis induced by ASPP2 depletion in mouse cancer cell 4T1 and human MDA-MB-231 promoted NETs formation vitro, as well lung metastases mice intravenously injected with ASPP2-deficient cells. Simvastatin berberine (BBR), synthesis inhibitors, efficiently blocked ASPP2-depletion formation. Cholesterol greatly enhanced Coiled-coil...
Abstract Abnormal energy metabolism is one of the characteristics tumours. In last few years, more and attention being paid to role regulation tumour aerobic glycolysis. Cancer cells display enhanced glycolysis, also known as Warburg effect, whereby absorb glucose produce a large amount lactic acid under conditions favour proliferation metastasis. this study, we report that haploinsufficient suppressor ASPP2, can inhibit HCC growth stemness by regulating effect through WNT/β‐catenin pathway....
Abstract Osteopontin (OPN) has been implicated widely in tumor growth and metastasis, but the range of its contributions is not yet fully understood. In this study, we show that genetic ablation Opn mice sensitizes them to diethylnitrosamine (DEN)-induced hepatocarcinogenesis. Opn-deficient (Opn−/− mice) exhibited enhanced production proinflammatory cytokines compensatory proliferation. Administering OPN antibody or recombinant protein wild-type Opn−/− mice-derived macrophages, respectively,...
It has been previously demonstrated that the 30-kDa Tat-interacting protein (TIP30) plays an important role in suppression of hepatocarcinogenesis by acting as a tumor suppressor. Here we report TIP30 suppresses metastasis hepatocellular carcinoma (HCC) through inhibiting transcription osteopontin (OPN), key molecule development metastasis. The expression messenger RNA was reverse to OPN HCC cell lines. Ectopic greatly suppressed expression, inhibited invasion cells extracellular matrix...
Abstract Purpose: To investigate Tip30 promoter methylation status in human hepatocellular carcinoma (HCC) and the correlation with clinicopathologic features prognosis. Experimental Design: The of CpG islands was examined 15 HCC cell lines as well 59 paired adjacent nontumor tissues. associations between survival patients were analyzed. Results: hypermethylated 6 10 reduced mRNA. DNA methyltransferase inhibitor, 5-aza-2′-deoxycytidine, greatly enhanced TIP30 expression sensitized cells to...
Abstract Cancer is, fundamentally, a disorder of cell growth and proliferation, which requires adequate supplies energy nutrients. In this study, we report that the haplo-insufficient tumor suppressor ASPP2, p53 activator, negatively regulates mevalonate pathway to mediate its inhibitory effect on in hepatocellular carcinoma (HCC). Gene expression profile analysis revealed key enzymes were increased when ASPP2 was downregulated. HCC cells gained higher cholesterol levels enhanced...