A5066016110- Protein Kinase Regulation and GTPase Signaling
- Melanoma and MAPK Pathways
- Minimally Invasive Surgical Techniques
- RNA modifications and cancer
- Renal cell carcinoma treatment
- Fungal and yeast genetics research
- Epigenetics and DNA Methylation
- Retinal Development and Disorders
- Cell death mechanisms and regulation
- Pediatric Urology and Nephrology Studies
- RNA Research and Splicing
- Renal and Vascular Pathologies
- Cancer-related Molecular Pathways
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- interferon and immune responses
- DNA Repair Mechanisms
- Surgical Simulation and Training
- Renal and related cancers
- Autophagy in Disease and Therapy
- Urological Disorders and Treatments
- Bladder and Urothelial Cancer Treatments
- Lung Cancer Treatments and Mutations
- NF-κB Signaling Pathways
- RNA Interference and Gene Delivery
Ideaya Biosciences (United States)
2024-2025
Washington University in St. Louis
2008-2024
Center for Systems Biology
2021-2024
The University of Texas Southwestern Medical Center
2011-2023
The University of Adelaide
1981-2023
Healthwise
2022
Urology San Antonio
2015-2022
University of Georgia
2021
Institute for Systems Biology
2021
Denver Health Medical Center
2020
Mitogen stimulation of cytoskeletal changes and c-jun amino-terminal kinases is mediated by Rac small guanine nucleotide-binding proteins. Vav, a guanosine diphosphate (GDP)-guanosine triphosphate (GTP) exchange factor for that stimulates the bound GDP GTP, to was directly controlled substrates products phosphoinositide (PI) 3-kinase. The PI 3-kinase substrate phosphatidylinositol-4,5-bisphosphate inhibited activation Vav tyrosine kinase Lck, whereas product...
Background: The recently identified RASSF1 locus is located within a 120-kilobase region of chromosome 3p21.3 that frequently undergoes allele loss in lung and breast cancers. We explored the hypothesis encodes tumor suppressor gene for Methods: assessed expression two products, RASSF1A RASSF1C, methylation status their respective promoters 27 non-small-cell cancer (NSCLC) cell lines, 107 resected NSCLCs, 47 small-cell (SCLC) 22 39 cancers, 104 nonmalignant samples, three epithelial...
Getting Autophagy to Akt The protein kinase is often activated in human cancers and thought promote tumor formation. One way which it may do so inhibit autophagy (a process by the cell digests its own proteins or organelles, especially damaged ones). Wang et al. (p. 956 , published online 25 October; see Perspective Koren Kimchi ) provide a direct molecular mechanism regulates autophagy. Beclin, component of machinery, appears be target phosphorylation Akt. Such enhanced interaction Beclin...
RNA interference (RNAi) is proving to be a robust and versatile technique for controlling gene expression in mammalian cells. To fully realize its potential vivo, however, it may necessary introduce chemical modifications optimize potency, stability, pharmacokinetic properties. Here, we test the effects of on stability inhibition expression. We find that duplexes containing either phosphodiester or varying numbers phosphorothioate linkages are remarkably stable during prolonged incubations...
Functional analysis of the proteome is an essential part genomic research. To facilitate different proteomic approaches, a MORF (moveable ORF) library 5854 yeast expression plasmids was constructed, each expressing sequence-verified ORF as C-terminal fusion protein, under regulated control. Analysis 5573 MORFs demonstrates that nearly all verified ORFs are expressed, suggests authenticity 48 characterized dubious, and implicates specific processes including cytoskeletal organization...
The RASSF1A locus at 3p21.3 is epigenetically inactivated high frequency in a variety of solid tumors.Expression sufficient to revert the tumorigenicity human cancer cell lines.We show here that can induce cycle arrest by engaging Rb family checkpoint.RASSF1A inhibits accumulation native cyclin D1, and RASSF1A-induced be relieved ectopic expression D1 or other downstream activators G 1 /S-phase transition (cyclin A E7).Regulation responsive activity, because RNA interference-mediated...
The RAS guanine nucleotide binding proteins activate multiple signaling events that regulate cell growth and differentiation. In quiescent fibroblasts, ectopic expression of activated H-RAS (H-RAS V12 , where indicates valine-12) induces membrane ruffling, mitogen-activated protein (MAP) kinase activation, stimulation DNA synthesis. A mutant H-RAS, V12C40 (where C40 cysteine-40), was identified defective for MAP activation synthesis, but retained the ability to induce ruffling. Another...
An excess risk of second malignancies has been reported in survivors Ewing's sarcoma. We examined a multiinstitutional data base to reevaluate the among sarcoma and identify possible causal factors.Information was derived from that included 266 Cumulative incidence rates were calculated. Contributions clinical features, type dose chemotherapy, cumulative radiation evaluated.After median follow-up duration 9.5 years (range, 3.0 30), 16 patients have developed malignancies, which 10 sarcomas...
Oncogenic Ras transforms cells through the activation of multiple downstream pathways mediated by separate effector molecules, one which is Raf. Here we report identification a second ras-binding protein that can induce cellular transformation in parallel with Raf/mitogen-activated kinase cascade. The Ral guanine nucleotide dissociation stimulator (RalGDS) was isolated from screen for Ras-binding proteins specifically interact effector-loop mutant, ras(12V,37G), uncouples Raf1. RalGDS, like...
Ral GTPases have been implicated in the regulation of a variety dynamic cellular processes including proliferation, oncogenic transformation, actin-cytoskeletal dynamics, endocytosis, and exocytosis. Recently Sec6/8 complex, or exocyst, multisubunit complex facilitating post-Golgi targeting distinct subclasses secretory vesicles, has identified as bona fide effector complex. regulate exocyst-dependent vesicle trafficking are required for exocyst assembly. Sec5, membrane-associated subunit,...